植物生态学报 ›› 2015, Vol. 39 ›› Issue (10): 1003-1011.DOI: 10.17521/cjpe.2015.0097

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柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析

王锦楠1,*, 陈进福2,*, 陈武生2, 周新洋3, 许东1, 李际红1,**(), 亓晓1   

  1. 1山东农业大学林学院农业生态与环境重点实验室, 山东泰安 271018
    2青海省林业科学研究所, 西宁 810016
    3山东大学药学院, 济南 250012
  • 出版日期:2015-10-01 发布日期:2015-10-24
  • 通讯作者: 王锦楠,陈进福,李际红
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    国家星火计划项目(2012GA870001)和青海省科技支撑计划(2012-N-1370)

Population genetic diversity of wild Lycium ruthenicum in Qaidam inferred from AFLP markers

WANG Jin-Nan1,*, CHEN Jin-Fu2,*, Chen Wu-Sheng1, Zhou Xin-Yang3, XU Dong1, LI Ji-Hong1,**(), QI Xiao1   

  1. 1Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Tai’an, Shandong 271018, China
    2Forestry Research Institute of Qinghai Province, Xining 810016, China
    3School of Pharmaceutical Sciences, Shandong University , Jinan 250012, China
  • Online:2015-10-01 Published:2015-10-24
  • Contact: Jin-Nan WANG,Jin-Fu CHEN,Ji-Hong LI
  • About author:

    # Co-first authors

摘要:

采用扩增片段长度多态性(AFLP)分子标记技术对青海省柴达木地区5个野生黑果枸杞(Lycium ruthenicum)种群的120份样品的遗传多样性进行分析。结果表明: 柴达木地区野生黑果枸杞具有很高的遗传多样性, 9对选扩引物共得到1691条清晰条带, 其中多态性条带1678条, 多态性变异率为99.23%, 种群间的有效等位基因数为1.4712, Nei’s基因多样性为0.3245, Shannon信息指数为0.4367。分子方差分析(AMOVA)结果表明: 柴达木地区5个黑果枸杞种群的遗传变异主要存在于种群内部(92%), 种群间的遗传分化较小(8%, 遗传分化系数0.08)。黑果枸杞种群间的遗传相似系数介于0.9709-0.9922之间, 平均值为0.9835。种群间的聚类及Mantel检验(γ = 0.3368, p = 0.8064)均表明柴达木地区黑果枸杞种群地理距离与遗传距离之间的相关性不明显; 黑果枸杞个体间的聚类表明同一种群的个体不能完全聚在一起。对同一种源的遗传多样性分析发现, 诺木洪奥斯勒草场的种源内部的遗传变异更为丰富, 这或许可以推断诺木洪可能为柴达木地区野生黑果枸杞种质资源的中心产区。

关键词: 黑果枸杞, 柴达木地区, 遗传多样性, AFLP

Abstract: <i>Aims</i>

Lycium ruthenicum is a perennial shrub plant. It grows among rocks in deserts, and its populations are spatially scattered in the distribution range. The objectives of this study were investigating the population genetic diversity and genetic structure of L. ruthenicum in Qaidam and determining relationships between genetic diversity and environmental factors.

<i>Methods</i>

We analyzed the genetic diversity of 120 individuals from 5 natural populations using amplified fragment length polymorphism (AFLP) markers.

<i>Important findings</i>

We obtained 1691 unambiguous bands from the nine pairs of selected primers, 1678 bands (99.23%) of which were polymorphic across all individuals. At the species level, the Nei’s gene diversity was 0.3649, and the Shannon’s information index was 0.5422. Analysis of molecular variance (AMOVA) analysis indicated that most variations (92.0%) existed within populations, with only a small proportion of total variations residing among populations (8.0%, genetic differentiation coefficient is 0.08). The genetic similarity among the populations ranged from 0.9709 to 0.9922 with an average of 0.9835. Results of the Mantel test (γ = 0.3368, p = 0.8064) and unweighted pair group method with arithmetic mean (UPGMA) cluster of the Qaidam populations indicated that the correlation between geographical distance and genetic distance was not significant. Principal coordinate analysis of all sampled individuals showed that individuals from each population did not cluster together. The genetic diversity of the same population indicated that the L. ruthenicum of Nomhon had high level of genetic variations, which indicated Nomhon is the center of the Qaidam wild L. ruthenicum populations.

Key words: Lycium ruthenicum, Qaidam, genetic diversity, AFLP