氮稳定同位素技术在陆地生态系统氮循环研究中的应用

doi:10.17521/cjpe.2019.0249

植物生态学报 ›› 2020, Vol. 44 ›› Issue (4): 373-383.DOI: 10.17521/cjpe.2019.0249

所属专题：稳定同位素生态学；生态学研究的技术和方法

氮稳定同位素技术在陆地生态系统氮循环研究中的应用

方运霆^1,^2,^3,^*(),刘冬伟^1,^2,³,朱飞飞^1,^2,³,图影^1,^3,⁴,李善龙^1,²,黄韶楠^1,^3,⁴,全智^1,^2,³,王盎^1,^2,³

¹中国科学院沈阳应用生态研究所森林生态与管理重点实验室, 沈阳 110016
²中国科学院清原森林生态系统观测研究站, 沈阳 110016
³辽宁省稳定同位素实验室, 沈阳 110016
⁴中国科学院大学, 北京 100049

收稿日期:2019-09-25 接受日期:2020-01-16 出版日期:2020-04-20 发布日期:2020-03-26
通讯作者: 方运霆
基金资助:
国家重点研发计划(2016YFA0600802);国家自然科学基金(41773094);中国科学院前沿科学重点研究项目(QYZDB-SSW-DQC002);中国科学院卢嘉锡国际团队项目(GJTD-2018-07)

Applications of nitrogen stable isotope techniques in the study of nitrogen cycling in terrestrial ecosystems

FANG Yun-Ting^1,^2,^3,^*(),LIU Dong-Wei^1,^2,³,ZHU Fei-Fei^1,^2,³,TU Ying^1,^3,⁴,LI Shan-Long^1,²,HUANG Shao-Nan^1,^3,⁴,QUAN Zhi^1,^2,³,WANG Ang^1,^2,³

¹Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016
²Qingyuan Forest CERN, Chinese Academy of Sciences, Shenyang 110016 China
³Key Laboratory of Stable Isotope Techniques and Applications, Liaoning Province, Shenyang, 110016
⁴University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-09-25 Accepted:2020-01-16 Online:2020-04-20 Published:2020-03-26
Contact: FANG Yun-Ting
Supported by:
National Key R&D Program of China(2016YFA0600802);National Natural Science Foundation of China(41773094);Key Research Program of Frontier Sciences of Chinese Academy of Sciences(QYZDB-SSW-DQC002);K.C.Wong Education Foundation(GJTD-2018-07)

摘要/Abstract

摘要：

在过去几十年中, 氮(N)稳定同位素技术的发展提高了人们对于陆地生态系统氮循环的认识。该文回顾了氮稳定同位素技术在研究生态系统氮循环中的历史, 综述了最近十多年来氮稳定同位素技术在陆地生态系统氮循环研究中的典型案例, 包括利用氮同位素自然丰度鉴定植物氮来源、指示生态系统氮状态和量化过程速率, 利用¹⁵N标记技术示踪氮的去向和再分布等。该文同时指出这些应用中存在的问题, 以及在陆地生态系统上氮稳定同位素技术今后研究的重点发展方向。

关键词: 氮稳定同位素技术, 自然丰度技术, ¹⁵N标记技术

Abstract:

In the past several decades, the development of nitrogen (N) stable isotope techniques has improved the understanding of N cycling in terrestrial ecosystems. This review briefly introduced the history of N stable isotope techniques in studying N cycling in terrestrial ecosystems and summarized typical studies focusing on different aspects of ecosystem N cycling in recent years, including using 1) ¹⁵N natural abundance to identify plant N sources, indicate N status of ecosystems, and quantify N transformation rates; 2) ¹⁵N enriched tracers to study N fates, redistribution and gaseous loss from ecosystems. In the end, this review points out challenges and future applications of N stable isotope techniques on studying N cycling in terrestrial ecosystems.

Key words: nitrogen stable isotope technique, natural abundance technique, ¹⁵N labelling technique

方运霆, 刘冬伟, 朱飞飞, 图影, 李善龙, 黄韶楠, 全智, 王盎. 氮稳定同位素技术在陆地生态系统氮循环研究中的应用. 植物生态学报, 2020, 44(4): 373-383. DOI: 10.17521/cjpe.2019.0249

FANG Yun-Ting, LIU Dong-Wei, ZHU Fei-Fei, TU Ying, LI Shan-Long, HUANG Shao-Nan, QUAN Zhi, WANG Ang. Applications of nitrogen stable isotope techniques in the study of nitrogen cycling in terrestrial ecosystems. Chinese Journal of Plant Ecology, 2020, 44(4): 373-383. DOI: 10.17521/cjpe.2019.0249

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2019.0249

https://www.plant-ecology.com/CN/Y2020/V44/I4/373

图/表 1

图1 氮循环过程中的同位素分馏效应(15ε, ‰, 修改自Denk et al., 2017)。英文字母代表生态系统氮循环过程: a, 矿化作用; b,硝化作用; c, 反硝化作用; d, 生物固氮; e, NH3和NH4+同位素交换; f, 硝酸盐异化还原成铵作用; g, 微生物固定; h, 植物吸收; i, 厌氧氨氧化作用; j, NH3挥发。

Fig. 1 Isotope fractionation effects in nitrogen processes (15ε, ‰, modified by Denk et al., 2017). Processes involved in terrestrial ecosystem N cycling are: a, mineralization; b, nitrification; c, denitrification; d, biological N2 fixation; e, isotope exchange between NH3 and NH4+; f, dissimilatory nitrate reduction to ammonium; g, immobilization by microbes; h, plant uptake; i, anaerobic ammonia oxidation; j, NH3 volatilization. SOM, soil organic matter.

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氮稳定同位素技术在陆地生态系统氮循环研究中的应用

Applications of nitrogen stable isotope techniques in the study of nitrogen cycling in terrestrial ecosystems

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