不同基因型茶菊对盐胁迫的响应

doi:10.3724/SP.J.1258.2013.00068

摘要/Abstract

摘要：

为探讨不同基因型茶菊(tea Chrysanthemum)在盐胁迫下的生理响应并对其进行耐盐性评价, 以4个不同基因型茶菊为材料, 采用营养液浇灌法, 研究了不同浓度NaCl (0、40、80、120、160、200 mmol·L^-1)胁迫下茶菊生理生化和光合生理响应特性。结果表明: 随着NaCl胁迫程度加大, 不同基因型茶菊叶片细胞膜透性(Cond)、丙二醛(MDA)含量、叶片脯氨酸(Pro)含量和可溶性糖(SS)含量增加; 超氧化物歧化酶(SOD)含量呈先升后降趋势; ‘乳荷’、‘黄滁龙’叶绿素(Chl)含量持续下降, ‘繁白露’和‘玉人面’叶绿素含量呈先升后降的趋势; 净光合速率(P_n)、蒸腾速率(T_r)和气孔导度(G_s)随NaCl胁迫浓度提高而极显著降低, 气孔限制值呈先升后降的趋势。采用隶属函数法对茶菊进行耐盐性评价, 不同基因型茶菊耐盐性由强到弱依次为‘乳荷’ > ‘玉人面’ > ‘繁白露’ > ‘黄滁龙’。其中, 耐盐性品种‘玉人面’、‘乳荷’在NaCl胁迫下, Chl含量、P_n、T_r和G_s下降幅度小, MDA含量和气孔限制值增幅较小。

关键词: NaCl胁迫, 光合生理, 生理响应, 耐盐性, 茶菊

Abstract:

Aims Our objective was to investigate the physiological response of tea Chrysanthemum cultivars to salt stress in order to select salt-tolerant cultivars for use in saline soil.
Methods Stress physiological, biochemical and photosynthetic physiological parameters were measured for four tea Chrysanthemum cultivars, which were irrigated with nutrient solution adding different concentrations of NaCl (40, 80, 120, 160 and 200 mmol·L^-1) as treatment and without addition of NaCl as control.
Important findings Following the increase with the degree of NaCl stress, data of relative membrane permeability (Cond), malonic aldehyde (MDA), proline (Pro) and soluble sugar (SS) content were increased in leaves of different genotypes of tea Chrysanthemum, while superoxide dismutase (SOD) activity was initially increased and then decreased. Leaf chlorophyll (Chl) content of the ‘Fanbailu’ and ‘Yurenmian’ cultivars were initially increased then decreased, but continued to decline in the ‘Ruhe’ and ‘Huangchulong’ cultivars. Net photosynthetic rate (P_n), transpiration rate (T_r) and stomatal conductance (G_s) were significantly reduced, but the stomatal limitation value was first increased and then decreased following the increase of salt concentrations. Values of subordinate function were used to evaluate the salt tolerance among different tea Chrysanthemum cultivars. The salinity tolerance for those cultivars was ‘Ruhe’ > ‘Yurenmian’ > ‘Fanbailu’ > ‘Huangchulong’. Under NaCl stress, parameters for Chl, P_n, T_r and G_s were decreased less in ‘Yurenmian’ and ‘Ruhe’ than in the other two cultivars while parameters for MDA content and stomatal limitation value were increased less in ‘Yurenmian’ and ‘Ruhe’ than in the other two cultivars.

Key words: NaCl stress, photosynthetic physiology, physiological response, salt tolerance, tea Chrysanthemum

吕晋慧,任磊,李艳锋,王玄,赵夏陆,张春来. 不同基因型茶菊对盐胁迫的响应. 植物生态学报, 2013, 37(7): 656-664. DOI: 10.3724/SP.J.1258.2013.00068

LÜ Jin-Hui,REN Lei,LI Yan-Feng,WANG Xuan,ZHAO Xia-Lu,ZHANG Chun-Lai. Responses to salt stress among different genotypes of tea Chrysanthemum. Chinese Journal of Plant Ecology, 2013, 37(7): 656-664. DOI: 10.3724/SP.J.1258.2013.00068

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2013.00068

https://www.plant-ecology.com/CN/Y2013/V37/I7/656

图/表 3

图1 NaCl胁迫对相对电导率、丙二醛含量、脯氨酸含量、可溶性糖含量、超氧化物歧化酶活性和叶绿素含量的影响(平均值±标准偏差)。不同大小写字母分别表示在p < 0.01和p < 0.05水平上差异显著。

Fig. 1 Effect of NaCl on relative conductivity, malonic aldehyde (MDA) content, proline content, soluble sugar content, superoxide dismutase (SOD) activity, chlorophyll content (mean ± SD). Different uppercase letters and lowercase letters indicate significant difference at p < 0.01 and p < 0.05 levels, respectively.

图2 NaCl胁迫对净光合速率、蒸腾速率、气孔导度和气孔限制值的影响(平均值±标准偏差)。不同字母表示差异显著(p < 0.01)。

Fig. 2 Effect of NaCl on net photosynthetic, transpiration rate, stomatal conductance, and stomatal limitation value (mean ± SD). Different letters indicate significant difference (p < 0.01).

表1 不同基因型茶菊隶属函数值、权重和综合评价值

Table 1 Value of subjection function, index weight and evaluation D of different genotypes of tea Chrysanthemum

品种 Cultivar	隶属函数值 Value of subjection function										综合值D Evaluation D	排序 Order
品种 Cultivar	Chl	Pro	Cond	SOD	SS	P_n	T_r	G_s	C_i	L_s	综合值D Evaluation D	排序 Order
‘乳荷’ ‘Ruhe’	0.498	0	1.000	0	0	0	0	0	0	0	0.227	1
‘玉人面’ ‘Yurenmian’	0	1.000	0.052	0.187	0.795	0.941	0.719	0.739	0.526	0.463	0.493	2
‘黄滁龙’ ‘Huangchulong’	1.000	0.967	0.351	1.000	1.000	1.000	1.000	0.963	1.000	0.835	0.904	4
‘繁白露’ ‘Fanbailu’	0.292	0.876	0	0.203	0.675	0.877	0.954	1.000	0.827	1.000	0.664	3
权重 Weight	0.100	0.054	0.031	0.204	0.107	0.065	0.071	0.055	0.104	0.045

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