植物生态学报 ›› 2008, Vol. 32 ›› Issue (5): 1015-1022.DOI: 10.3773/j.issn.1005-264x.2008.05.006

• 研究论文 • 上一篇    下一篇

光温交叉处理对小麦紫黄质脱环氧化酶活性及其热耗散能力的影响

陈华新1,2, 陈玮1,2, 姜闯道3, 高辉远1,2,*(), 邹琦1,2   

  1. 1 山东农业大学生命科学学院,山东泰安 271018
    2 作物生物学国家重点实验室,山东泰安 271018
    3 中国科学院植物研究所,北京 100093
  • 收稿日期:2006-07-17 接受日期:2008-01-16 出版日期:2008-07-17 发布日期:2008-09-30
  • 通讯作者: 高辉远
  • 作者简介:*(gaohy@sdau.edu.cn)
  • 基金资助:
    国家自然科学基金(30571125);国家自然科学基金(30671451);高校博士点专项基金(20050434007)

EFFECTS OF TEMPERATURE AND LIGHT TREATMENT ON VIOLAXANTHIN DE-EPOXIDASE ACTIVITY AND XANTHOPHYLL CYCLE-DEPENDENT ENERGY DISSIPATION IN WHEAT LEAVES

CHEN Hua-Xin1,2, CHEN Wei1,2, JIANG Chuang-Dao3, GAO Hui-Yuan1,2,*(), ZOU Qi1,2   

  1. 1College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong 271018, China
    2State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong 271018, China
    3Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2006-07-17 Accepted:2008-01-16 Online:2008-07-17 Published:2008-09-30
  • Contact: GAO Hui-Yuan

摘要:

为了探讨温度和光强是如何影响离体紫黄质脱环氧化酶(VDE)活性, 阐明依赖叶黄素循环的热耗散与VDE活性关系, 该文以小麦(Triticum aestivum)为材料, 研究了不同光强(200、500、900和1 200 μmol·m -2·s-1)和不同温度(4、25、38和45 ℃) 交叉处理对小麦叶片VDE活性以及依赖叶黄素循环热耗散能力的影响。结果表明: 小麦叶片VDE活性在30 ℃最高, 说明30 ℃是小麦叶片VDE体外条件下的最适温度; 不同光强处理下小麦叶片VDE活性基本一致。与室温(25 ℃)处理的叶片相比, 低温(4 ℃)处理的叶片VDE活力没有明显下降, 而高温(45 ℃)处理则导致了叶片VDE活性急剧下降。小麦叶片热耗散(NPQ)以及依赖叶黄素循环的热耗散(qE)均随着处理光强的增加不断上升, 而qE/NPQ则随光强增加略微下降, 在1 200 μmol·m -2·s-1光强条件下qE/NPQ则急剧下降。该研究揭示VDE活性与依赖叶黄素循环热耗散能力的指标qE/NPQ的变化有一定的相关性, 但不完全一致。并针对此问题进行了讨论。

关键词: 叶黄素循环, 紫黄质脱环氧化酶, 光抑制, 热耗散

Abstract:

Aims Our objective was to explore how temperature and light directly affect activity of violaxanthin de-epoxidase (VDE) in vitro and to clarify the relationship between VDE activity and xanthophyll cycle-dependant energy dissipation.

Methods We investigated VDE activities and energy dissipation in wheat leaves treated with different temperatures (4, 25, 38 and 45 ℃) combined with different light intensities (200, 500, 900 and 1 200 μmol·m -2·s-1).

Important findings Maximum activity of VDE in wheat leaves appeared at 30 ℃, suggesting that this was the optimum temperature for VDE in vitro. Light intensity had no effects on VDE activity in wheat leaves in vitro. Compared with 25 ℃, 4 ℃ treatment exhibited no obvious effect on VDE activity; however, 38 ℃ treatment caused slight increase while 45 ℃ caused dramatic decrease in the VDE activity. With increasing light intensity, non-photochemical quenching (NPQ) and xanthophyll cycle-dependent energy dissipation (qE) increased significantly. However, the ratio qE/NPQ decreased slightly when the temperature increased from 4 ℃ to 38 ℃ and decreased significantly when the temperature was increased to 45 ℃. Under low and moderate light intensity, the VDE activity measured at treatment temperature was in agreement with the qE/NPQ, which is a measure of the capacity of xanthophyll cycle-dependent energy dissipation.

Key words: xanthophyll cycle, violaxanthin de-epoxidase (VDE), photoinhibition, energy dissipation