植物生态学报 ›› 2008, Vol. 32 ›› Issue (5): 1015-1022.DOI: 10.3773/j.issn.1005-264x.2008.05.006
陈华新1,2, 陈玮1,2, 姜闯道3, 高辉远1,2,*(), 邹琦1,2
收稿日期:
2006-07-17
接受日期:
2008-01-16
出版日期:
2008-07-17
发布日期:
2008-09-30
通讯作者:
高辉远
作者简介:
*(gaohy@sdau.edu.cn)基金资助:
CHEN Hua-Xin1,2, CHEN Wei1,2, JIANG Chuang-Dao3, GAO Hui-Yuan1,2,*(), ZOU Qi1,2
Received:
2006-07-17
Accepted:
2008-01-16
Online:
2008-07-17
Published:
2008-09-30
Contact:
GAO Hui-Yuan
摘要:
为了探讨温度和光强是如何影响离体紫黄质脱环氧化酶(VDE)活性, 阐明依赖叶黄素循环的热耗散与VDE活性关系, 该文以小麦(Triticum aestivum)为材料, 研究了不同光强(200、500、900和1 200 μmol·m -2·s-1)和不同温度(4、25、38和45 ℃) 交叉处理对小麦叶片VDE活性以及依赖叶黄素循环热耗散能力的影响。结果表明: 小麦叶片VDE活性在30 ℃最高, 说明30 ℃是小麦叶片VDE体外条件下的最适温度; 不同光强处理下小麦叶片VDE活性基本一致。与室温(25 ℃)处理的叶片相比, 低温(4 ℃)处理的叶片VDE活力没有明显下降, 而高温(45 ℃)处理则导致了叶片VDE活性急剧下降。小麦叶片热耗散(NPQ)以及依赖叶黄素循环的热耗散(qE)均随着处理光强的增加不断上升, 而qE/NPQ则随光强增加略微下降, 在1 200 μmol·m -2·s-1光强条件下qE/NPQ则急剧下降。该研究揭示VDE活性与依赖叶黄素循环热耗散能力的指标qE/NPQ的变化有一定的相关性, 但不完全一致。并针对此问题进行了讨论。
陈华新, 陈玮, 姜闯道, 高辉远, 邹琦. 光温交叉处理对小麦紫黄质脱环氧化酶活性及其热耗散能力的影响. 植物生态学报, 2008, 32(5): 1015-1022. DOI: 10.3773/j.issn.1005-264x.2008.05.006
CHEN Hua-Xin, CHEN Wei, JIANG Chuang-Dao, GAO Hui-Yuan, ZOU Qi. EFFECTS OF TEMPERATURE AND LIGHT TREATMENT ON VIOLAXANTHIN DE-EPOXIDASE ACTIVITY AND XANTHOPHYLL CYCLE-DEPENDENT ENERGY DISSIPATION IN WHEAT LEAVES. Chinese Journal of Plant Ecology, 2008, 32(5): 1015-1022. DOI: 10.3773/j.issn.1005-264x.2008.05.006
图1 酶促反应体系的温度对紫黄质脱环氧的酶(VDE)活性的影响 每个点为3~4次测定的平均值(mean ± SE, n=3 or 4)
Fig. 1 Effect of various temperatures on violaxanthin de-epoxidase (VDE) activities
图2 在不同温度下, 小麦叶片的PSⅡ实际光化学效率ФPSⅡ对光强的响应(a)以及在不同光强下 小麦叶片的ΦPSⅡ对温度的响应(b) 叶片在不同光照和温度下处理4 h, 每个点为5~6次测定的平均值 Samples were exposed to different light intensities and temperatures for 4 h (means ± SE, n=5 to 6)
Fig. 2 Response of actual photochemical efficiency of PSⅡ (ΦPSⅡ ) to light intensity at different temperatures (a) and to temperatures under different light conditions (b)
图3 在不同温度下小麦叶片PSⅡ最大光化学效率Fv/Fm对光强的响应(a)以及在不同光强下 小麦叶片的Fv/Fm对温度的响应(b) 图注同图2
Fig. 3 Response of maximum photochemical efficiency of PSⅡ (Fv/Fm ) to light intensity at different temperatures (a) and to temperatures under different light conditions (b) Notes see fig.2
图4 在不同温度下小麦叶片的非光化学淬灭NPQ、依赖叶黄素循环的热耗散qE对光强的响应(a、c)以及在不同光强下小麦叶片对温度的响应(b、d) 图注同图2 Notes see
Fig. 4 Response of non-photochemical quench NPQ, energy dissipation depending on xanthophyll cycle qEto light intensity at different temperatures (a, c) and to temperature under different light conditions (b, d)
图5 在不同温度下小麦叶片的qE /NPQ (a)对光强的响应 以及在不同光强下qE/NPQ对温度的相应(b) 叶片在不同光照和温度下处理4 h。每个点为5~6次测定的平均值
Fig. 5 Response of qE/NPQ in wheat leaves to light intensities at different temperatures (a) and temperatures at different light conditions (b). Samples were exposed to different light intensity and temperature for 4 h ( means ± SE, n=5 to 6)
图6 测定温度与处理温度相同条件下(a), 以及在VDE的最适温30 ℃下(c), 小麦叶片紫黄质脱环氧的酶(VDE)活性对光强的响应;测定温度与处理温度相同条件下(b), 以及在VDE的最适温30 ℃下(d)小麦叶片的VDE活性对温度的响应 图注同图2 Notes see
Fig. 6 Response of violaxanthin de-epoxidase (VDE) activities to light intensity at different temperatures (a、c) and temperatures at different light conditions (b、d)
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