植物生态学报 ›› 2010, Vol. 34 ›› Issue (2): 144-150.DOI: 10.3773/j.issn.1005-264x.2010.02.005

所属专题: 稳定同位素生态学

• 稳定同位素生态学专题论文 • 上一篇    下一篇

毛白杨杂种无性系叶片δ13C差异与气体交换参数

何茜3,1, 李吉跃3,1,*(), 沈应柏2, 陈晓阳3, 尚富华1, 胡磊1, 张志毅2,*()   

  1. 1北京林业大学省部共建森林培育与保护教育部重点实验室, 北京 100083
    2北京林业大学林木花卉遗传与育种教育部重点实验室, 北京 100083
    3华南农业大学林学院, 广州 510642
  • 收稿日期:2008-11-07 接受日期:2009-01-25 出版日期:2010-11-07 发布日期:2010-02-01
  • 通讯作者: 李吉跃,张志毅
  • 作者简介:Zhangzy@bjfu.edn.cn
    * E-mail: ljyymy@vip.sina.com;

Difference in δ13C and gas exchange parameters among Populus tomentosa clones

HE Qian3,1, LI Ji-Yue3,1,*(), SHEN Ying-Bai2, CHEN Xiao-Yang3, SHANG Fu-Hua1, HU Lei1, ZHANG Zhi-Yi2,*()   

  1. 1Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China
    2Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing 100083, China
    3College of Forestry, South China Agricultural University, Guangzhou 510642, China
  • Received:2008-11-07 Accepted:2009-01-25 Online:2010-11-07 Published:2010-02-01
  • Contact: LI Ji-Yue,ZHANG Zhi-Yi

摘要:

在苗木生长的不同时期对13个毛白杨(Populus tomentosa)杂种无性系叶片碳同位素δ13C和气体交换参数(净光合速率Pn、蒸腾速率Tr、瞬时水分利用效率WUEi、气孔导度Gs和胞间CO2浓度Ci)的差异进行研究, 分析不同无性系间δ13C与气体交换参数的相互关系, 目的在于探求δ13C在筛选高光合及高水分利用效率毛白杨杂种无性系中的应用价值。结果表明: 不同生长时期和不同无性系间δ13C、TrWUEiGsCi的差异均显著, δ13C和WUEi表现为9月>7月, TrGsCi表现为7月>9月, Pn在不同生长时期差异不显著。季节变化是引起毛白杨杂种无性系叶片δ13C差异的主要原因。同一时期, 无性系间δ13C和WUEi表现出较好的一致性, 即WUEi较高的无性系30、42、46、83、BL2和BL5, 其δ13C值也较高, WUEi较低的无性系B331和TG34, 其δ13C值也较低, 且不同时期(7月和9月) δ13C和WUEi呈较强的正相关, 相关系数r分别为0.739 0和0.545 8, 高δ13C可以作为筛选高WUEi毛白杨的有效指标, 且在苗木生长旺盛时期选育能得到更为可靠的结果。对毛白杨而言, 高WUEi的无性系, 一般具有适中或较低的GsCi, 但不一定具有很高的Pn, 气孔调节使得毛白杨在不影响光合作用的同时保持较高的WUE

关键词: δ13C, 无性系, 气体交换参数, 毛白杨, 水分利用效率

Abstract:

Aims Fast, efficient breeding of fast-growth and high water use efficiency (WUE) in Populus tomentosa clones is a pressing need for afforestation in arid and semi-arid regions. There is much research on the correlation between stable carbon isotope and WUE in crops and a few trees, but little in different clones of P. tomentosa. Our objective was to explore the application of δ13C on selection of clones with high photosynthesis and WUE.

Methods We examined 13 clones of Populus-grafted seedlings in Beijing Forestry University (39°46′ N, 116°19′ E) Nursery in Beijing, China. We studied leaf δ13C of the seedlings by MAT-251-MS in two different growth periods (mid-July and September) and gas exchange parameters (net photosynthetic rate (Pn), transpiration rate (Tr), instantaneous water use efficiency (WUEi), stomatal conductance (Gs) and intercellular CO2 concentration (Ci)) by LI-6400 portable photosynthesis system between 9:30 and 11:30 a.m. in the same periods.

Important findings Leaf δ13C, Tr, WUEi, Gs and Ci significantly differed between the periods and among clones. Leaf δ13C and WUEi were higher in July than September; however, Tr, Gs and Ci were lower in July than September and Pn showed no significant difference between periods. The main reason for the difference of leaf δ13C was seasonal variation. Leaf δ13C and WUEi of the clones were consistent during the same period, i.e, the higher WUEi appeared in clones of 30, 42, 46, 83, BL2 and BL5 with higher δ13C and the lower WUEi occurred in clones of B331 and TG34 with lower δ13C. There was strong positive correlation between δ13C and WUEi (r = 0.739 0 and 0.545 8 in July and September, respectively); therefore, high δ13C can be used as an effective indicator of high WUEi in Populus. Clones with higher WUEi may have moderate or low Gs and Ci, but not necessarily high Pn.

Key words: δ13C, clones, gas exchange parameters, Populus tomentosa, water use efficiency