西土寒宪蚓和三叉苦植物对大叶相思人工林土壤CO2通量的短期效应

doi:10.3773/j.issn.1005-264x.2010.11.001

摘要/Abstract

摘要：

西土寒宪蚓(Ocnerodrilus occidentalis)为广东人工林和撂荒地内广泛分布的外来种蚯蚓, 因其对水热、pH值及土壤有机质等的变化不敏感, 其分布范围有逐渐扩大的趋势。研究西土寒宪蚓对人工林碳循环的影响过程, 可以为如何减少外来蚯蚓影响下的人工林土壤碳排放提供思路。在广东鹤山大叶相思(Acacia auriculaeformis)人工林内设置外来蚯蚓和乡土植物野外控制实验, 利用静态箱-气相色谱法对土壤CO₂通量进行15天的原位测定。结果发现, 单独添加西土寒宪蚓及单独种植三叉苦(Evodia lepta), 对土壤CO₂通量的效应都不明显。植物物理过程(如遮阴作用等)、植物生物过程(如根际化学物质分泌过程等)及植物在未添加蚯蚓样方和添加蚯蚓样方中对土壤CO₂通量的效应分别为-32.1%、40.9%、8.8%和-7.2%、30.7%、23.5%。植物的物理过程抑制了土壤CO₂排放, 但提高了蚯蚓对土壤CO₂排放的促进作用(提高了39.3%)。植物的生物过程促进了土壤CO₂排放, 但减弱了蚯蚓对土壤CO₂排放的促进作用(降低了23.5%)。试验期间蚯蚓对多数土壤理化性质的影响并不明显, 但是蚯蚓的存在有增强土壤细菌活性的趋势, 而且使土壤CO₂通量与土壤理化性质的相关性更加密切了; 同时, 蚯蚓的存在也使土壤CO₂通量与土壤水热因子的关系发生了变化。可见, 森林土壤CO₂通量不仅与水热条件有关, 还受地上和地下生物过程的调控。如果只关注森林土壤CO₂通量的大小, 而忽略影响土壤CO₂产生及释放的生物学过程, 将无法找到减少森林土壤CO₂排放的有效途径。减缓人工林中土壤碳的排放, 必须综合考虑植物物理过程、植物生物过程以及蚯蚓对土壤CO₂排放过程的独立效应和交互效应。

关键词: CO₂, 蚯蚓效应, 假植物, 微生物, 胁迫指数, 植物物理效应

Abstract:

Aims The exotic earthworm Ocnerodrilus occidentalis is widespread in plantation and abandoned areas in Guangdong, China. Its distribution is gradually expanding due to insensitivity to temperature, moisture, soil pH and soil organic matter. Study of the processes of soil carbon dynamics affected by O. occidentalis can provide new insights for the reduction of soil carbon emissions. Our objective was to investigate the short-term impacts of this exotic earthworm and native plants on soil CO₂ fluxes.
Methods A field experiment was conducted in an Acacia auriculaeformis plantation at the Heshan Hilly Land Interdisciplinary Experimental Station. CO₂fluxes were measured for 15 days in situ using the static chamber technique and analyzed with a gas chromatogram.
Important findings Both O. occidentalis and Evodia lepta had no significant effects on soil CO₂fluxes. The effects of plant physical processes (such as shading), plant biological processes (such as secretion of root exudates) and overall processes on soil CO₂ fluxes were -32.1%, 40.9% and 8.8%, respectively, in treatments without earthworm addition, and were -7.2%, 30.7% and 23.5%, respectively, in treatments with earthworm addition. Plant physical processes inhibited soil CO₂ emissions, but enhanced the effects of the earthworm on soil CO₂ emissions (increased by 39.3%). Plant biological processes enhanced soil CO₂emissions, but inhibited the effects of earthworms on soil CO₂ emissions (decreased by 23.5%). Earthworm addition showed almost no significant impacts on most soil physical and chemical properties, but enhanced the activity of soil bacteria and led to a closer correlation between soil CO₂fluxes and soil physical and chemical properties. Meanwhile, earthworm activities changed the relationships between soil CO₂ fluxes and soil hydrothermal factors. Hence, soil CO₂ fluxes were not only influenced by hydrothermal factors, but also regulated by above- and below-ground biological processes. Therefore, it was difficult to determine an effective way to reduce forest soil CO₂ emissions if only the amount of soil CO₂ is considered and the impact of plant biological processes on soil CO₂ fluxes is ignored. In order to reduce soil carbon efflux, it will be useful to note the potential of the independent and interactive effects of plant physical processes, plant biological processes and earthworm activity on soil CO₂ flux.

Key words: carbon dioxide, earthworm effect, fake plant, microorganisms, stress index, physical effect of plant

高波, 张卫信, 刘素萍, 邵元虎, 熊燕梅, 周存宇, 傅声雷. 西土寒宪蚓和三叉苦植物对大叶相思人工林土壤CO₂通量的短期效应. 植物生态学报, 2010, 34(11): 1243-1253. DOI: 10.3773/j.issn.1005-264x.2010.11.001

GAO Bo, ZHANG Wei-Xin, LIU Su-Ping, SHAO Yuan-Hu, XIONG Yan-Mei, ZHOU Cun-Yu, FU Sheng-Lei. Short-term impacts of Ocnerodrilus occidentalis and Evodia lepta on soil CO₂fluxes in an Acacia auriculaeformis plantation in Guangdong Province, China. Chinese Journal of Plant Ecology, 2010, 34(11): 1243-1253. DOI: 10.3773/j.issn.1005-264x.2010.11.001

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https://www.plant-ecology.com/CN/Y2010/V34/I11/1243

图/表 8

表2 土壤CO2通量与土壤理化因素之间关系的回归模型(y = b0 + b1x1 + b2x2 + b3x3 + b4x4 + b5x5)

Table 2 Regression models for the relationship between soil carbon dioxide fluxes and soil physical and chemical characteristics (y = b0 + b1x1 + b2x2 + b3x3 + b4x4 + b5x5)

回归方程 Regression equation		b₀	b₁	b₂	b₃	b₄	b₅	p	R²
CO₂通量 CO₂ flus	y (B)	484.2	2 479.3	-114.8	6.39	-9.55	-92.56	0.052	0.47
CO₂通量 CO₂ flus	y (A)	2 360.1	917.3	-4.80	4.27	-28.23	-573.5	0.046	0.50

图1 不同处理下土壤CO2通量的比较(平均值±标准误差, n = 56)。不同字母表示不同处理在p < 0.05水平上的差异显著。CK, 对照; CK + E, 添加西土寒宪蚓; FP, 种植假植物; FP + E, 种植假植物并添加西土寒宪蚓; P, 种植植物; P + E, 种植植物并添加西土寒宪蚓。

Fig. 1 Comparison of soil carbon dioxide fluxes under different treatments (mean ± SE, n = 56). Different letters on column indicate significant differences (p < 0.05) between treatments, which were performed by two-way ANOVA analysis. CK, control; CK + E, plot with earthworm; FP, plot with fake plant; FP + E, plot with fake plant and earthworm; P, plot with plant; P + E, plot with plant and earthworm.

图2 不同处理土壤CO2通量的时间动态(平均值±标准误差)。CK、CK + E、FP、FP + E、P和P + E同图1。

Fig. 2 Temporal dynamics of soil carbon dioxide fluxes under different treatments (mean ± SE). CK, CK + E, FP, FP + E, P and P + E see Fig. 1.

表1 西土寒宪蚓及植物对土壤CO2通量效应的计算公式(%)

Table 1 Formulas of earthworm and plant effects on soil carbon dioxide fluxes (%)

	无西土寒宪蚓 Without Ocnerodrilus occidentalis	有西土寒宪蚓 With O. occidentalis	蚯蚓效应 Earthworm effects
植物物理效应 Physical effect of plant	(FP - CK)/CK × 100	(FPE - CKE)/CKE × 100	(FPE - FP)/FP × 100
植物生物效应 Biological effect of plant	(P - FP)/CK × 100	(PE - FPE)/CKE × 100	[(PE - P)-(FPE - FP)]/(P - FP) × 100
植物总效应 Plant effects	(P - CK)/CK × 100	(PE - CKE)/CKE × 100	(PE - P)/P × 100
对照土壤 Control soil	-	-	(CKE - CK)/CK × 100

图3 各处理土壤理化性质比较。*, 处理间差异达到显著水平(p < 0.05)。CK、CK + E、FP、FP + E、P、P + E同图1。

Fig. 3 The physical and chemical properties of surface soil under different treatments. *, difference between treatments was significant (p < 0.05). CK, CK + E, FP, FP + E, P, P + E see Fig.1.

表3 土壤CO2通量与地表温度、湿度和土壤5 cm处温度及0-5 cm土壤平均湿度之间关系的回归模型(y = b0e(b1T0 + b2T5)W0b3W5b4) (n = 56)

Table 3 Regression models for the relationship between soil carbon dioxide fluxes, soil surface temperature and moisture & soil 5 cm temperature and moisture content (0-5 cm) under different treatments (y = b0e(b1T0 + b2T5)W0b3W5b4) (n = 56)

处理	b₀	b₁	b₂	b₃	b₄	p	R²
CK	122 412	0.098	-0.165	-0.704	-0.557	0.000	0.55
CK + E	675	0.027	0.013	-0.658	0.203	0.429	0.07
FP	2913	-0.189	0.212	-0.789	0.144	0.075	0.16
FP + E	98	0.140	-0.110	-0.330	0.281	0.053	0.20
P	5209	-0.069	0.111	-0.567	-0.387	0.000	0.34
P + E	2	0.118	-0.034	0.570	-0.005	0.034	0.19

图4 不同处理磷脂脂肪酸总量、真菌细菌比、真菌和细菌磷脂脂肪酸量及细菌胁迫指数比较(平均值±标准误差)。CK、CK + E、FP、FP + E、P、P + E同图1。

Fig. 4 Comparisons of total phospholipids fatty acids (PLFAs), Fungi : bacteria ratio, fungal PLFAs, bacteria PLFAs and bacteria stress index under different treatments (mean ± SE). CK, CK + E, FP, FP + E, P, P + E see Fig. 1.

图5 蚯蚓及植物对土壤CO2通量作用效应简图。+, 对CO2通量的正效应; –, 对CO2通量的负效应; 0, 无效应或效应很弱; ?, 未知作用过程。

Fig. 5 Mechanistic diagram of earthworm and plant effects and their interaction on soil carbon dioxide flux. +, positive effects; –, negative effects; 0, no effect or weak effect; ?, unknown process.

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西土寒宪蚓和三叉苦植物对大叶相思人工林土壤CO₂通量的短期效应

Short-term impacts of Ocnerodrilus occidentalis and Evodia lepta on soil CO₂fluxes in an Acacia auriculaeformis plantation in Guangdong Province, China

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