植物生态学报 ›› 2017, Vol. 41 ›› Issue (7): 787-794.DOI: 10.17521/cjpe.2016.0322

• 方法与技术 • 上一篇    下一篇

一种适合野外使用的被子植物分子标本干燥方式

沈风娇, 任倩倩, 董琦, 朱丽, 张建芳, 杨婧, 张冉, 梁红柱, 赵建成, 石硕*()   

  1. 河北师范大学生命科学学院, 石家庄 050024
  • 收稿日期:2016-10-17 接受日期:2017-06-01 出版日期:2017-07-10 发布日期:2017-08-21
  • 通讯作者: 石硕
  • 作者简介:

    * 通信作者Author for correspondence (E-mail:shishuo@hebtu.edu.cn)

  • 基金资助:
    地方高校国家级大学生创新创业训练计划(201510094011)

A new angiosperms molecular specimen treatment method for field use

Feng-Jiao SHEN, Qian-Qian REN, Qi DONG, Li ZHU, Jian-Fang ZHANG, Jing YANG, Ran ZHANG, Hong-Zhu LIANG, Jian-Cheng ZHAO, Shuo SHI*()   

  1. College of Life Science, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2016-10-17 Accepted:2017-06-01 Online:2017-07-10 Published:2017-08-21
  • Contact: Shuo SHI
  • About author:

    KANG Jing-yao(1991-), E-mail: kangjingyao_nj@163.com

摘要:

该研究通过比较不同干燥方式对被子植物分子标本的影响, 试图得到一种野外采集过程中可以替代硅胶干燥法的更为便捷的植物分子标本的干燥方法。选取40、80和150 ℃烘干和吸水纸压制干燥、硅胶干燥法对日本晚樱(Prunus serrulata var. lannesiana)和山麦冬(Liriope spicata)两种植物的新鲜叶片进行干燥处理, 提取各种处理样品的DNA, 并将DNA进行电泳检测、分光光度计检测及PCR扩增, 以此评价不同的干燥方式对植物基因组DNA的影响。分光光度计检测及总DNA电泳结果显示, 经40 ℃烘干或硅胶干燥处理的样品总DNA浓度及长片段DNA浓度较其他干燥方式高; PCR产物浓度统计学分析显示, 40 ℃烘干处理的样品PCR产物浓度高于其他干燥方式。基于以上结果, 建议在野外采集被子植物分子标本时, 使用40 ℃烘干干燥法对分子标本进行干燥处理, 避免分子标本快速降解以及硅胶干燥法的污染问题, 同时可节省携带、更换大量硅胶材料所耗费的人力。

关键词: 被子植物, 野外, 分子标本, DNA, 烘干, 硅胶

Abstract:

Aims This study aims to identify a more convenient drying method for obtaining molecular specimen of angiosperms in the field than the conventional silica gel drying method. Methods The leaves of Prunus serrulata var. lannesiana and Liriope spicata were dried under temperatures of 150 °C, 80 °C, 40 °C as well as under natural conditions, and by the silica gel drying method, respectively. The DNA extracts of various specimens were then analyzed using techniques of spectrophotometer detection, electrophoresis and PCR to evaluate the impacts of different drying treatments to the genomic DNA of testing plants. Important findings The concentrations of total DNA were higher for the specimens dried at 40 °C treatment and by the silica gel drying method than other treatments when assessed by the techniques of spectrophotometer detection and electrophoresis. The concentration of PCR products was highest in the specimens dried at 40 °C. Based on the results, the 40 °C drying can be recommended for obtaining molecular specimens of angiosperms because of its minimum degree of degradation, for convenience of operation and avoiding carrying large amounts of silica gel in field investigations.

Key words: angiosperms, field, molecular specimen, DNA, drying, silica gel