Chin J Plan Ecolo ›› 2005, Vol. 29 ›› Issue (6): 1038-1042.doi: 10.17521/cjpe.2005.0132

• Research Articles • Previous Articles    

INFLUENCE OF METHANOL ELUATES FRACTIONATED FROM MACADAMIA INTEGRIFOLIAROOTS ON SPORE GERMINATION AND HYPHAL GROWTH OF AM FUNGI

LIU Jian_Fu1, YANG Dao_Mao1, OUYANG Ming_An1, WANG Li_Na1, ZHANG Yong2, and ZENG Ming3   

  1. 1 Department of Bioengineering, HuaQiao University, Quanzhou, Fujian 362021, China)(2 College of Life Sciences, Nankai University, Tianjin 300071, China) (3 College of Horticulture, Southwest University, Chongqing 400716, China)
  • Online:2005-09-30 Published:2005-09-30
  • Contact: ZHANG Yong

Abstract:

Macadamia integrifoliaoriginated from Australia where it grows in the rainforests of the eastern coastal areas of the Northern Rivers district of New South Wales and South_East Queensland. The Macadamiatree belongs to Proteaceae. The Macadamiawas in fact the only native Australian plant ever developed to a commercial food crop. Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with the roots of approximately 90% of terrestrial plant species. These fungi are an important component of ecosystems, and the diversity of the AMF could largely influence plant biodiversity, ecosystem variability and productivity.  We examined the role of M. integrifolia root exudates in stimulating the germination and growth of AMF. In September 2003, 100 g of M. integrifolia roots were sampled, dried and crushed. The roots were then extracted for two hours in a 70% methanol solution, stirred in 80 ℃ water and filtered. The extract was diluted to 20%, 40%, 60%, 80% and 100% with methanol. The AMF cultures were sterilized, and the spores of Gornas mosseae and Gigaspora margarita were inoculated and cultured at 25 ℃ in the dark for 20 days. The number of spores that germinated was counted and the length of the mycelium measured. When the concentration of the extract was diluted by 60% methanol, spore germination of Gornas mosseae and Gigaspora margarita reached maximum levels of 81.7% and 76.0%, respectively. At higher extract dilution levels, spore germination decreased, and at 100% dilution, spore germination was lower than 65%. The mycelium reached maximum lengths when the extract was diluted by 60%. The length of Gornas mosseaewas 31.2 mm and Gigaspora margarita was 28.0 mm. Mycelium growth declined when the extract dilution increased, especially at 100% dilution. At lower dilutions of 20%, the growth of Gornas mosseae appeared to be stimulated but no effect on Gigas poramargarita growth was observed. At the zero percent dilution, the promotion of mycelium growth was not observed. The concentration of flavones in the extract was the greatest in the 60% methanol dilution (19.26%) but was lower at lower dilutions and only 2.56% in the zero percent dilution. Flavone concentrations also decreased at higher dilutions and the content of flavones was lower than 80% at 100% dilution. There were significant positive correlations (correlation coefficient > 0.95) between the concentration of flavones in the extract and spore germination and mycelium growth of AMF.

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