Chin J Plan Ecolo ›› 2015, Vol. 39 ›› Issue (12): 1166-1175.doi: 10.17521/cjpe.2015.0113

• Orginal Article • Previous Articles     Next Articles

Short-term nitrogen fertilization decreased root and microbial respiration in a young Cunninghamia lanceolata plantation

WANG Qing-Kui1,2,*, LI Yan-Peng1,3, ZHANG Fang-Yue1,3, HE Tong-Xin1,3   

  1. 1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
    2Huitong National Research Station of Forest Ecosystem, Huitong, Hunan 418307, China
    and 3University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2015-12-31 Published:2015-12-01
  • Contact: Qing-Kui WANG
  • About author:

    # Co-first authors

Abstract:

Aims As the primary pathway for CO2 emission from terrestrial ecosystems to the atmosphere, soil respiration is estimated to be 80 Pg C·a-1 to 100 Pg C·a-1, equivalent to 10 fold of fossil fuel emissions. As an important management practice in plantation forests, fertilization does not only increase primary production but also affects soil respiration. To investigate how nitrogen (N) fertilization affects total soil, root and microbial respiration, a N fertilization experiment was conducted in a five-year-old Cunninghamia lanceolata plantation in Huitong, Hunan Province, located in the subtropical region. MethodsOne year after fertilization, soil respiration was monitored monthly by LI-8100 from July 2013 to June 2014. Soil temperature and water content (0-5 cm soil depth) were also measured simultaneously. Available soil nutrients, fine root biomass and microbial communities were analyzed in June 2013. Important findings Total soil, root and microbial respiration rates were 22.7%, 19.6%, and 23.5% lower in the fertilized plots than in the unfertilized plots, respectively. The temperature sensitivity (Q10) of soil respiration ranged from 1.81 to 2.04, and the Q10 value of microbial respiration decreased from 2.04 in the unfertilized plots to 1.84 in the fertilized plots. However, neither the Q10 value nor the patterns of total soil respiration were affected by N fertilization. In the two-factor model, soil temperature and moisture accounted for 69.9%-79.7% of the seasonal variations in soil respiration. These results suggest that N fertilization reduces the response of soil organic carbon decomposition to temperature change and may contribute to the increase of soil carbon storage under global warming in subtropical plantations.

Key words: Cunninghamia lanceolata plantation, soil carbon emission, heterotrophic respiration, nitrogen fertilization, temperature sensitivity

Table 1

Property of soils (0-10 cm) and growth conditions of young Cunninghamia lanceolata plantation before fertilization"

全碳 Total C
(g·kg-1)
全氮 Total N (g·kg-1) 全磷 Total P (g·kg-1) pH 容重 Bulk density (g·cm-3) 树高
Tree height (m)
胸径 Diameter at breast height (cm)
对照 Control 17.2 1.43 0.339 4.50 1.28 5.71 8.27
施氮 N fertilization 18.6 1.55 0.334 4.33 1.22 6.01 8.64

Fig. 1

Influence of N fertilization and root trenching on soil temperature and water content (0-5 cm) in a young Cunninghamia lanceolata plantation (mean ± SD)."

Table 2

Effect of N fertilization on soil nutrient availability, biomass, C and N content of fine roots in a young Cunninghamia lanceolata plantation"

矿质氮
Mineral N (mg·kg-1)
有效磷
Available P (mg·kg-1)
pH 细根生物量
Fine root biomass (g·m-2)
细根碳氮含量 C and N content in fine root (g·kg-1)
碳 C 氮 N C:N
对照 Control 4.94 4.93 4.39 151.1 439.5 6.28 69.9
施氮 N fertilization 13.16 6.49 4.11 102.6 436.2 8.80 49.6
p <0.001 >0.05 <0.01 <0.05 >0.05 <0.01 <0.01

Table 3

Effect of N fertilization on soil microbial biomass C (MBC) and microbial community composition in a young Cunninghamia lanceolata plantation"

微生物生物量碳
MBC
(mg·kg-1)
细菌
Bacteria (nmol·g-1)
真菌
Fungi
(nmol·g-1)
细菌:真菌
Bacteria:fungi
放线菌
Actinomyces
(nmol·g-1)
革兰氏阳性细菌
Gram-positive bacteria (nmol·g-1)
对照 Control 234 26.25 10.61 2.47 3.99 12.09
施氮 N fertilization 155 19.94 6.76 2.95 3.55 8.90
p <0.001 <0.05 <0.05 >0.05 >0.05 <0.05

Fig. 2

Influence of N fertilization on total soil, microbial and root respiration in a young Cunninghamia lanceolata plantation (mean ± SD)."

Table 4

Parameters of different soil respiration models in a young Cunninghamia lanceolata plantation"

处理
Treatment
Rs = aebT Rs = aW + b Rs = aebTWc
a b R2 a b R2 a b c R2
土壤总呼吸
Total soil respiration
对照 Control 0.474 0.067 0.714 -0.022 2.242 0.055 0.182 0.070 0.308 0.712
施氮 N fertilization 0.425 0.059 0.694 -0.018 1.760 0.065 0.165 0.066 0.288 0.699
微生物呼吸
Microbial respiration
对照 Control 0.335 0.071 0.795 -0.023 1.920 0.101 0.156 0.067 0.273 0.797
施氮 N fertilization 0.319 0.061 0.721 -0.052 1.161 0.010 0.117 0.064 0.314 0.738

Fig. 3

Annual flux of total soil, microbial and root respiration in a young Cunninghamia lanceolata plantation (mean ± SD)."

Fig. 4

Relationship of total soil and microbial respiration with soil temperature in the fertilized and unfertilized plots."

Fig. 5

Effect of N fertilization on the temperature sensitivity (Q10) of total soil and microbial respiration in a young Cunninghamia lanceolata plantation (mean ± SD)."

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