Chin J Plan Ecolo ›› 2015, Vol. 39 ›› Issue (12): 1209-1215.doi: 10.17521/cjpe.2015.0117

• Orginal Article • Previous Articles    

Exogenous polyamines alleviating salt stress on peanuts (Arachis hypogaea) grown in pots

MENG De-Yun1,2,3, HOU Lin-Lin2,3,4, YANG Sha2,3, MENG Jing-Jing2,3, GUO Feng2,3, LI Xin-Guo2,3,*(), WAN Shu-Bo3,*()   

  1. 1School of Life Science of Shandong University, Jinan 250100, China
    2Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China
    3Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China
    and 4College of Agro- nomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China
  • Online:2015-12-31 Published:2015-12-01
  • Contact: Xin-Guo LI,Shu-Bo WAN E-mail:lixinguo@tom.com;wansb@saas.ac.cn
  • About author:

    # Co-first authors

Abstract:

Aims Soil salinity is a major limiting factor for plant establishment, development and productivity. In recent years, the contradiction between oil crops and food crops for land is increasingly prominent. In order not to take up the land for food, peanut planting on saline-alkali land could be a promising option. However, peanuts have been rarely grown in saline-alkali land, which may be due to the reduction of peanut yield caused by salt stress. Therefore, research of peanut salt resistance has important practical significance.Methods In order to investigate the effects of exogenous polyamines on peanut (Arachis hypogaea) grown in pots under salt stress, ‘Huayu 22’, one of the peanut cultivars, was used as materials by being foliar-sprayed with 1 mmol·L-1 putrescine (Put), 1 mmol·L-1 spermidine (Spd) and 1 mmol·L-1 spermine (Spm) to elucidate the role of exogenous polyamines on peanuts under 150 mmol·L-1NaCl. Important findingsResults showed that growth, yield, chlorophyll contents and antioxidant enzyme activities of peanut seedling decreased, however, malondialdehyde (MDA) content and relative electrolytic leakage increased under salt stress. Meanwhile, exogenous polyamines significantly improved the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduced the relative electrolytic leakage and MDA content in peanut leaves under salt stress and thus alleviating the oxidative damage of salt stress on plasma membrane. It is obvious that exogenous polyamines could improve chlorophyll contents, plant height, number of branch and the amount of dry matter accumulation, even pod yield under salt stress. Among these three polyamines, the effects of exogenous Spm on alleviating salt stress were most effective These results showed that exogenous polyamines, especially Spm, were favorable for the seedlings to increase reactive oxygen metabolism and photosynthesis, which improved peanut growth and reduced the inhibitory effects of salt stress on peanuts.

Key words: antioxidant enzyme, peanut, polyamines, salt stress, yield

Table 1

Effects of exogenous polyamines on agronomic characters of Arachis hypogaea seedlings under salt stress (mean ± SD)"

处理
Treatment
主茎高
Plant height (cm)
侧枝长
Branch length (cm)
分枝数
Branch number
CK 31.33 ± 0.63a 32.17 ± 0.31a 14.33 ± 0.24a
NaCl 26.50 ± 0.25c 27.00 ± 0.48b 9.67 ± 0.24c
NaCl + Put 27.67 ± 0.29bc 30.50 ± 0.85ab 11.67 ± 0.47b
NaCl + Spd 29.33 ± 0.52b 29.25 ± 0.64ab 12.67 ± 0.24ab
NaCl + Spm 29.67 ± 0.58b 31.50 ± 0.38a 11.67 ± 0.24b

Fig. 1

Effects of exogenous polyamines on total biomass of Arachis hypogaea under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1."

Fig. 2

Effects of exogenous polyamines on leaf chlorophyll content of Arachis hypogaea under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1."

Fig. 3

Effects of exogenous polyamines on superoxide dismutase (SOD) activity (A), peroxidase (POD) activity (B) and catalse (CAT) activity (C) of Arachis hypogaea leaves under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1."

Fig. 4

Effects of exogenous polyamines on relative electrolytic leakage (A) and malondialdehyde (MDA) content (B) of Arachis hypogaea leaves under salt stress (mean ± SD). FE, FP, PP and PF represent flower early stage, flower-pegging stage, pod-setting stage and pod filling stage, respectively. Put, Spd, Spm see Table 1."

Table 2

Effects of exogenous polyamines on the yield of Arachis hypogaea under salt stress (mean ± SD)"

处理
Treatment
产量
Pods mass per plant (g)
饱果数
Full fruits number per plant
总果数
Total pod number per plant
果针数
Gynophores number per plant
CK 47.57 ± 1.59a 29.67 ± 0.67a 39.70 ± 1.33a 67.00 ± 2.00a
NaCl 35.50 ± 0.93c 16.21 ± 0.33c 30.67 ± 2.67b 40.67 ± 1.48d
NaCl + Put 39.17 ± 0.87bc 17.67 ± 0.33bc 31.00 ± 0.67b 43.00 ± 2.67cd
NaCl + Spd 40.25 ± 1.13b 21.00 ± 1.24b 31.67 ± 0.48b 45.00 ± 1.67c
NaCl + Spm 41.50 ± 1.32b 20.67 ± 0.67b 39.33 ± 1.24a 57.00 ± 3.33b
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