Chin J Plan Ecolo ›› 2016, Vol. 40 ›› Issue (12): 1289-1297.doi: 10.17521/cjpe.2016.0215

• Research Articles • Previous Articles     Next Articles

Correlation analysis between vein density and stomatal traits of Robinia pseudoacacia in different aspects of Beishan Mountain in Lanzhou

Bei-Bei DUAN, Cheng-Zhang ZHAO*, Ting XU, Hui-Ling ZHENG, Wei FENG, Ling HAN   

  1. Research Center of Wetland Resources Protection and Industrial Development Engineering of Gansu Province, College of Geography and Environmental Science, Northwest Normal University, Lanzhou 730070, China
  • Online:2016-12-30 Published:2016-12-31
  • Contact: Cheng-Zhang ZHAO

Abstract: AimsThe relationship between leaf venation and stomatal traits reflects leaf water potential of plants, and is fundamental to probe the relationship between physiological functions and water use. Our objective was to study the changes in the relationship between venation density and stomatal traits (stomatal density and stomatal length) in Robinia pseudoacacia with the changes in slope aspects.MethodsIn Beishan Mountain of Lanzhou in Gansu Province, China, 20 transects were laid out horizontally along the contour at intervals of 50 m from an elevation of 1550-1750 m in four different slope aspects, and 12 plots were set up along each transect at intervals of 5 m. A handed GPS (global positioning system) was used to measure latitude, longitude and altitude at each plot. Community characteristics, including crown density, tree height and soil moisture, were investigated. Robinia pseudoacacia within all plots were sampled and used for measurements of individual leaf area, venation density, stomatal density and stomatal length in laboratory in each plot. The 240 plots were categorized into groups of southern, eastern, western and northern aspects, and the standardized major axis (SMA) estimation method was then used to examine the relationship between the stomatal density, stomatal length and vein density.Important findings The results showed that with a change of the aspect from south to east, west, and north, the crown density, average tree height and soil moisture of the plant community increased, while leaf net photosynthetic rate (Pn), transpiration rate (Tr), photosynthetically active radiation (PAR), vein density and stomatal density decreased. In addition, stomatal length and individual leaf area increased. Venation density of each aspect of R. pseudoacacia was positively correlated with stomatal density (p < 0.05) and negatively with stomatal length (p < 0.05). These correlations were strongest in south slope (p < 0.01).

Key words: Robinia pseudoacacia, vein density, stomatal density, stomatal length, slope

Table 1

Main characteristics of plots in different slope aspects (mean ± SE)"

坡向 Aspect 群落特征
Community characteristics
Soil moisture
Crown density (%)
Height (cm)
南坡 South slope 67.38 ± 2.71c 301.72 ± 15.14c 6.83 ± 0.51c
东坡 East slope 79.09 ± 4.01b 341.03 ± 13.22b 7.91 ± 0.38b
西坡 West slope 78.77 ± 5.29b 339.45 ± 11.99b 7.84 ± 0.41b
北坡 North slope 87.15 ± 4.91a 398.55 ± 23.35a 8.77 ± 0.29a

Table 2

Leaf traits and photosynthetic characteristics of Robinia pseudoacacia in different slope aspects (mean ± SE)"

南坡 South slope 东坡 East slope 西坡 West slope 北坡 North slope
叶脉密度 Vein density (cm·cm-2) 195.74 ± 9.80a 177.19 ± 8.56b 166.59 ± 8.33b 150.01 ± 7.50c
气孔密度 Stomatal density (No.·mm-2) 238.16 ± 11.90a 224.74 ± 11.24b 229.85 ± 11.49b 214.77 ± 11.74c
气孔大小 Stomatal length (μm) 7.89 ± 0.39c 8.76 ± 0.44b 8.71 ± 0.44b 9.64 ± 0.48a
单叶叶面积 Individual leaf area (cm2) 5.18 ± 0.26c 6.27 ± 0.31b 6.46 ± 0.32b 7.61 ± 0.38a
PAR (μmol·m-2·s-1) 1 024.51 ± 51.22a 925.34 ± 46.27b 920.57 ± 46.03b 780.48 ± 39.02c
Pn (μmol CO2·m-2·s-1) 6.24 ± 0.31a 5.02 ± 0.25b 4.88 ± 0.25b 4.35 ± 0.22c
Tr (mmol H2O·m-2·s-1) 5.81 ± 0.29a 4.78 ± 0.24b 4.62 ± 0.23b 3.89 ± 0.19c

Fig. 1

Relationship between vein density and stomatal density of Robinia pseudoacacia in different aspects. A, South slope. B, East slope. C, West slope. D, North slope. y and x is two parameter: log stomatal density and log vein density, yA = 0.39x + 1.50 represents the logarithms of the linear relationship between stomatal density and vein density, the rest can be done in the same manner."

Fig. 2

Relationship between vein density and stomatal length of Robinia pseudoacacia in different aspects. A, South slope. B, East slope. C, West slope. D, North slope. y and x is two parameter: log stomatal length and log vein density, yA = -0.16x + 1.25 represents the logarithms of the linear relationship between stomatal length and vein density, the rest can be done in the same manner."

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[1] SHI Yuan-Chun,ZHAO Cheng-Zhang,SONG Qing-Hua,DU Jing,CHEN Jing,WANG Ji-Wei. Slope-related variations in twig and leaf traits of Robinia pseudoacacia in the northern mountains of Lanzhou [J]. Chin J Plan Ecolo, 2015, 39(4): 362-370.
[2] WU Xu,CHEN Yun-Ming,TANG Ya-Kun. Sap flow characteristics and its responses to precipitation in Robinia pseudoacacia and Platycladus orientalis plantations [J]. Chin J Plan Ecolo, 2015, 39(12): 1176-1187.
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