Effects of soil nitrogen and phosphonium on leaf nitrogen and phosphonium stoichiometric characteristics and chlorophyll content of Oligostachyum lubricum

doi:10.3724/SP.J.1258.2011.01219

Abstract

Abstract:

Aims Organ stoichiometric characteristics are the bridge that connects environment and plant organ traits. The relationships among environment, organ stoichiometric characteristics and organ traits reveal mechanisms of environmental effects on plant organ traits and make it possible to regulate plant traits. Our objective was try to find the relationships among soil and leaf nitrogen (N), phosphonium (P) stoichiometric characteristics and leaf chlorophyll content for Oligostachyum lubricum.
Methods Total N, P concentrations of the original soil in pots were 421.76 and 37.35 mg·kg^-1, respectively, and the original soil was treated as the control (1N1P). Total experimental N, P concentration were two, three and four times as high as the control. Different N, P levels were combined into 10 combinations (2N2P, 2N3P, 2N4P, 3N2P, 3N3P, 3N4P, 4N2P, 4N3P, 4N4P and 1N1P) and every combination except the control was achieved by adding different amounts of NH₄NO₃ and NH₄H₂PO₄. Leaf samples were collected from ramets of O. lubricum after grown in the pot soil with different N, P level combinations for 45 days. Leaf total N concentration was determined by employing the Kjeldahl method and leaf total P concentration by the acid melt-molybdenum stibium anti-color method. Leaf chlorophyll concentrations were measured based on acetone-ethanol mixture (1 : 1) extraction method. Soil and leaf total N, P concentrations were expressed as mg·kg^-1DW.
Important findings Soil total N concentration was significantly positively correlated with leaf total N concentration and leaf N : P ratio, whereas soil total P concentration had no significant correlation with leaf total P concentration and leaf N : P ratio. Leaf N : P ratio increased with the increasing of soil N : P ratio, and the rate of increase of soil N : P ratio was faster than that of leaf N : P ratio. At the same soil condition, leaf N : P ratio of ramets growing in soil with 2N2P and 3N3P had no significant difference, but the both were higher than the control (1N1P) and lower than that growing in soil with 4N4P. Leaf N : P was the main factor that affected leaf chlorophyll content. Results suggested that soil total N concentration had more effect on leaf N, P stoichiometric characteristics than soil total P. Sufficient supply of soil total N lead to the luxury uptake of N by leaves of O. lubricum. The growth of O. lubricum was limited by low soil total N concentration before N and P addition.

Key words: chlorophyll, ecological stoichiometry, luxury uptake, nitrogen and phosphorus, Oligostachyum lubricum

GU Da-Xing, CHEN Shuang-Lin, HUANG Yu-Qing. Effects of soil nitrogen and phosphonium on leaf nitrogen and phosphonium stoichiometric characteristics and chlorophyll content of Oligostachyum lubricum[J]. Chin J Plant Ecol, 2011, 35(12): 1219-1225.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.01219

https://www.plant-ecology.com/EN/Y2011/V35/I12/1219

Figures/Tables 5

References 34

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土壤N、P水平 Soil N, P level	土壤全N Soil total N (mg·kg^-1)	土壤全P Soil total P (mg·kg^-1)	土壤N : P Soil N : P
1N1P	421.76	37.35	11.29
2N2P	843.52	74.70	11.29
2N3P	843.52	112.05	7.53
2N4P	843.52	149.41	5.65
3N2P	1265.29	74.70	16.94
3N3P	1265.29	112.05	11.29
3N4P	1265.29	149.41	8.47
4N2P	1687.05	74.70	22.58
4N3P	1687.05	112.05	15.06
4N4P	1687.05	149.41	11.29

土壤N、P水平 Soil N, P level	土壤全N Soil total N (mg·kg^-1)	土壤全P Soil total P (mg·kg^-1)	土壤N : P Soil N : P
1N1P	421.76	37.35	11.29
2N2P	843.52	74.70	11.29
2N3P	843.52	112.05	7.53
2N4P	843.52	149.41	5.65
3N2P	1265.29	74.70	16.94
3N3P	1265.29	112.05	11.29
3N4P	1265.29	149.41	8.47
4N2P	1687.05	74.70	22.58
4N3P	1687.05	112.05	15.06
4N4P	1687.05	149.41	11.29

	叶片叶绿素含量 Leaf chlorophyll content	叶片N : P Leaf N : P	土壤N : P Soil N : P	土壤N Soil N	土壤P Soil P	叶片N Leaf N
叶片N : P Leaf N : P	0.590^**	1.000
土壤N : P Soil N : P	0.347	0.476^**	1.000
土壤N Soil N	0.505^**	0.804^**	0.569^**	1.000
土壤P Soil P	0.130	0.317	-0.516^**	0.375^*	1.000
叶片N Leaf N	0.462^*	0.643^**	0.190	0.703^**	0.573^**	1.000
叶片P Leaf P	-0.511^**	-0.917^**	-0.459^*	-0.660^**	-0.159	-0.329

	叶片叶绿素含量 Leaf chlorophyll content	叶片N : P Leaf N : P	土壤N : P Soil N : P	土壤N Soil N	土壤P Soil P	叶片N Leaf N
叶片N : P Leaf N : P	0.590^**	1.000
土壤N : P Soil N : P	0.347	0.476^**	1.000
土壤N Soil N	0.505^**	0.804^**	0.569^**	1.000
土壤P Soil P	0.130	0.317	-0.516^**	0.375^*	1.000
叶片N Leaf N	0.462^*	0.643^**	0.190	0.703^**	0.573^**	1.000
叶片P Leaf P	-0.511^**	-0.917^**	-0.459^*	-0.660^**	-0.159	-0.329

影响因子 Influencing factors	直接效应 Direct effect	间接效应 Indirect effect					相关系数 Correlation coefficient
影响因子 Influencing factors	直接效应 Direct effect	X₁→Y	X₂→Y	X₃→Y	X₄→Y	总计 Total	相关系数 Correlation coefficient
X₁	0.312		0.012	0.130	0.137	0.278	0.590^**
X₂	0.015	0.251		0.140	0.099	0.490	0.505^**
X₃	0.202	0.201	0.010		0.049	0.260	0.462^*
X₄	-0.149	-0.286	-0.010	-0.066		-0.362	-0.511^**