Chin J Plant Ecol ›› 2012, Vol. 36 ›› Issue (3): 262-268.DOI: 10.3724/SP.J.1258.2012.00262

• Research Articles • Previous Articles    

Effect of degrading bacterium HN36 on ultrastructure of flue-cured tobacco stem and leaves under quinclorac stress

LIU Hua-Shan1,2(), ZUO Tao2, HAN Jin-Feng2,**(), XU Shu-Xia1, ZHANG Zhi-Yong2, WANG Xiao-Jun2, YUAN Shi-Hao3, PU Wen-Xuan3, YI Jian-Hua3,**()   

  1. 1College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China
    2College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
    3Technology Center of Hunan Tobacco Industry Co. Ltd., Changsha 410014, China
  • Received:2011-07-06 Accepted:2011-11-10 Online:2012-07-06 Published:2012-02-28
  • Contact: HAN Jin-Feng,YI Jian-Hua

Abstract:

Aims Our objectives were to determine (a) the effects of quinclorac-degrading bacterium on ultrastructure of tobacco stem and leaves in quinclorac-contaminated soil and (b) the degradation of quinclorac in soil after bacterium HN36 treatment. The research will provide a theoretical basis for soil remediation by degrading bacteria and for tobacco production safety.
Methods We treated quinclorac-contaminated soil with degrading bacterium HN36 and then observed the ultrastructure of tobacco leaves using an electron microscope and determined chemical composition.
Important findings Cell ultrastructures of stem and tip leaves were more affected by quinclorac than those of middle leaves. The degrading bacterium HN36 had a positive effect on ultrastructure of tobacco leaves. The degradation of quinclorac in soil treated with the degrading bacterium was significantly faster than in untreated soil. Applying quinclorac-degrading bacterium HN36 to the soil contaminated by quinclorac could mitigate phytotoxicity of the tobacco cell ultrastructure and accelerate the degradation of quinclorac in soil.

Key words: degrading bacterium HN36, quality, quinclorac, tobacco, ultrastructure