Chin J Plan Ecolo ›› 2013, Vol. 37 ›› Issue (5): 454-463.doi: 10.3724/SP.J.1258.2013.00047

• Research Articles • Previous Articles     Next Articles

Physiological responses to drought stress and the emission of induced volatile organic compounds in Rosmarinus officinalis

LIU Fang1, ZUO Zhao-Jiang1, XU Gai-Ping1, WU Xing-Bo1, ZHENG Jie1, GAO Rong-Fu2, ZHANG Ru-Min1, and GAO Yan1*   

  1. 1The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China;

    2College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
  • Received:2012-12-27 Revised:2013-03-14 Online:2013-05-16 Published:2013-05-01
  • Contact: GAO Yan


Aims Drought is one of the numerous environmental factors which affect the growth and development of plants, and it becomes more severe in many regions of the world due to climate change. To examine the mechanisms of responses of the plant Rosemarinus offcinalis to drought stress, we measured the physiological and biochemical changes and volatile organic compounds (VOCs) emission of 2-year-old seedlings of this species under conditions of light drought (LD), intermediate drought (MD) and severe drought (SD).
Methods The VOCs emission was measured using the dynamic headspace air-circulation method, and the composition and content of VOCs were analyzed using the thermal desorption system/gas chromatography/mass spectrometer technique (TDS-GC-MS). We measured ion leakage of cell membranes and the content of soluble sugar, soluble protein and malondialdehyde (MDA), activities of lipoxygenase and protective enzymes of R. offcinalis under the different drought treatments.
Important findings The content of osmotic adjustment materials in R. offcinalis leaves was significantly influenced by drought stress. The content of soluble sugar was increased by 51.5% and 87.4% (p < 0.01) under MD and SD stresses after 12 days, respectively. The content of soluble protein was increased by 82% and 140% (p < 0.01), respectively. There were differences among the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in response to drought stress, which was a coordination reaction of those three enzymes to the stress. With prolonged drought stress, the MDA content (p < 0.01) and cell injury rate (p < 0.05) were increased significantly. Terpenoids were the main components of R. offcinalis VOCs and their relative content was more than 46% of the total VOCs. Compared with the control, it was increased by 14.4%, 17.0% and 23.7%, respectively, under LD, MD and SD stresses, respectively. Meanwhile, green leaf volatiles (GLVs) and aldehydes were markedly induced by drought stress, and (E)-2-hexenal, leaf alcohol, sorbaldehyde and n-decanal were newly found under the drought stress. The results indicated that R. offcinalis could enhance its drought-resistant ability by adjusting activities of protective enzymes, improving contents of osmotic adjustment materials and releasing VOCs.

[1] Apel K, Hirt H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–399. Crossref
[2] Ashraf M, Iram A (2005). Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora-Morphology, Distribution, Functional Ecology of Plants, 200, 535–546. Crossref
[3] Atkinson NJ, Urwin PE (2012). The interaction of plant biotic and abiotic stresses: from genes to the field. Journal of Experimental Botany, 63, 3523–3543. Crossref
[4] Baldwin IT, Halitschke R, Paschold A, von Dahl CC, Preston CA (2006). Volatile signaling in plant-plant interactions: “Talking trees” in the genomics era. Science, 311, 812– 815. Crossref
[5] Bengough AG, McKenzie BM, Hallett PD, Valentine TA (2011). Root elongation, water stress, and mechanical im- pedance: a review of limiting stresses and beneficial root tip traits. Journal of Experimental Botany, 62, 59–68. Crossref
[6] Blanch JS, Peñuelas J, Llusià J (2007). Sensitivity of terpene emissions to drought and fertilization in terpene-storing Pinus halepensis and non-storing Quercus ilex. Physiologia Plantarum, 131, 211–225. Crossref
[7] Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.
[8] Chance B, Maehly AC (1955). Assay of catalase and peroxidase. Methods in Enzymology, 2, 764–775.
[9] Chaves MM, Oliveira MM (2004). Mechanisms underlying plant resilience to water deficits: prospects for water- saving agriculture. Journal of Experimental Botany, 55, 2365–2384. Crossref
[10] Cramer GR, Urano K, Delrot S, Pezzotti M, Shinozaki K (2011). Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biology, 11, 163. Crossref
[11] Delfine S, Loreto F, Pinelli P, Tognetti R, Alvino A (2005). Isoprenoids content and photosynthetic limitations in rosemary and spearmint plants under water stress. Agriculture, Ecosystems & Environment, 106, 243–252. Crossref
[12] Dicke M, Loreto F (2010). Induced plant volatiles: from genes to climate change. Trends in Plant Sciences, 15, 115–117. Crossref
[13] Dudareva N, Pichersky E, Gershenzon J (2004). Biochemistry of plant volatiles. Plant Physiology, 135, 1893–1902. Crossref
[14] Farooq M, Wahid A, Ahmad N, Asad SA (2010). Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associa- ted metabolic events. Paddy and Water Environment, 8, 15–22. Crossref
[15] Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29, 185–212. Crossref
[16] Filella I, Peñuelas J, Seco R (2009). Short-chained oxygenated VOC emissions in Pinus halepensis in response to changes in water availability. Acta Physiologiae Plantarum, 31, 311–318. Crossref
[17] Gao Y, Jin YJ, Li HD, Chen HJ (2005). Volatile organic compounds and their roles in bacteriostasis in five conifer species. Journal of Integrative Plant Biology, 47, 499–507. Crossref
[18] Gao Y, Zhu YZ, Yang ZM, Du HM (2012). Effects of drought stress and recovery on antioxidant enzyme activities of Agropyron cristatum. Acta Agrectir Sinica, 20, 336–341. (in Chinese with English abstract) [高悦, 朱永铸, 杨志民, 杜红梅 (2012). 干旱胁迫和复水对冰草相关抗性生理指标的影响. 草地学报, 20, 336–341. ] Crossref
[19] Gershenzon J, Dudareva N (2007). The function of terpene natural products in the natural world. Nature Chemical Biology, 3, 408–414. Crossref
[20] Giannopolitis CN, Ries SK (1977). Superoxide dismutase: I. Occurrence in higher plants. Plant Physiology, 59, 309–314.
[21] Gill SS, Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909–930. Crossref
[22] Gouinguené SP, Turlings TCJ (2002). The effects of abiotic factors on induced volatile emissions in corn plants. Plant Physiology, 129, 1296–1307. Crossref
[23] Grote R, Lavoir AV, Rambal S, Staudt M, Zimmer I, Schnitzler JP (2009). Modelling the drought impact on monoterpene fluxes from an evergreen Mediterranean forest canopy. Oecologia, 160, 213–223. Crossref
[24] Grote R, Keenan T, Lavoir AV, Staudt M (2010). Process- based simulation of seasonality and drought stress in monoterpene emission models. Biogeosciences, 7, 257– 274. Crossref
[25] Hatanaka A (1993). The biogeneration of green odour by green leaves. Phytochemistry, 34, 1201–1218.
[26] Holopainen JK, Gershenzon J (2010). Multiple stress factors and the emission of plant VOCs. Trends in Plant Science, 15, 176–184. Crossref
[27] Huang HM, Guo ZJ, Lu RM, Meng L (2012). Optimization of extraction technology of volatile oil from rosemary and analysis on the chemical constituents of volatile oil. Hubei Agricultural Sciences, 51, 2321–2324. (in Chinese with English abstract) [黄宏妙, 郭占京, 卢汝梅, 蒙亮 (2012). 迷迭香挥发油提取工艺优化及其化学成分分析. 湖北农业科学, 51, 2321–2324.] Crossref
[28] Ibrahim MA, Maenpaa M, Hassinen V, Kontunen-Soppela S, Malec L, Rousi M, Pietikainen L, Tervahauta A, Karenlampi S, Holopainen JK, Oksanen EJ (2010). Elevation of night-time temperature increases terpenoid emissions from Betula pendula and Populus tremula. Journal of Experimental Botany, 61, 1583–1595. Crossref
[29] Jaleel CA, Manivannan P, Wahid A, Farooq M, Al-Juburi HJ, Somasundaram R, Vam RP (2009). Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11, 100–105.
[30] Jaspers P, Kangasjärvi J (2010). Reactive oxygen species in abiotic stress signaling. Physiologia Plantarum, 138, 405–413. Crossref
[31] Jin YQ, Li DL, Chen XX, Zhang LJ (2012). Physiological re- sponse of Sapium sebiferum seedlings from different provenances to drought stress. Acta Botanica Boreali- Occidentalia Sinica, 32, 1395–1402. (in Chinese with English abstract) [金雅琴, 李冬林, 陈小霞, 张丽娟 (2012). 不同种源乌桕幼苗对干旱胁迫的生理响应. 西北植物学报, 32, 1395–1402.] Crossref
[32] Laothawornkitkul J, Paul ND, Vickers CE, Possell M, Taylor JE, Mullineaux PM, Hewitt CN (2008). Isoprene emissions influence herbivore feeding decisions. Plant, Cell & Environment, 31, 1410–1415. Crossref
[33] Lavoir AV, Staudt M, Schnitzler JP, Landais D, Massol F, Rocheteau A, Rodriguez R, Zimmer I, Rambal S (2009). Drought reduced monoterpene emissions from Quercus ilex trees: results from a throughfall displacement experiment within a forest ecosystem. Biogeosciences Discussions, 6, 863–893. Crossref
[34] Loreto F, Schnitzler JP (2010). Abiotic stresses and induced BVOCs. Trends in Plant Science, 15, 154–166. Crossref
[35] Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2010). Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell & Environment, 33, 453–467. Crossref
[36] Niinemets U, Loreto F, Reichstein M (2004). Physiological and physicochemical controls on foliar volatile organic com- pound emissions. Trends in Plant Science, 9, 180–186. Crossref
[37] Patakas A, Nikolaou N, Zioziou E, Radoglou K, Noitsakis B (2002). The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines. Plant Science, 163, 361–367.
[38] Pinheiro C, Chaves MM, Ricardo CP (2001). Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of Lupinus albus L. Journal of Experimental Botany, 52, 1063–1070. Crossref
[39] Potters G, Pasternak TP, Guisez Y, Jansen MAK (2009). Different stresses, similar morphogenic responses: integrating a plethora of pathways. Plant, Cell & Environment, 32, 158–169. Crossref
[40] Simpraga M, Verbeeck H, Demarcke M, Joó É, Pokorska O, Amelynck C, Schoon N, Dewulf J, van Langenhove H, Heinesch B, Aubinet M, Laffineur Q, Müller JF, Steppe K (2011). Clear link between drought stress, photosynthesis and biogenic volatile organic compounds in Fagus sylvatica L. Atmospheric Environment, 45, 5254–5259. Crossref
[41] Staudt M, Ennajah A, Mouillot F, Joffre R (2008). Do volatile organic compound emissions of Tunisian cork oak populations originating from contrasting climatic conditions differ in their responses to summer drought? Canadian Journal of Forest Research, 38, 2965–2975. Crossref
[42] Sui XY, Liu TT, Ma CH, Yang Z, Zu YG, Zhang L, Wang H (2012). Microwave irradiation to pretreat rosemary (Rosmarinus officinalis L.) for maintaining antioxidant content during storage and to extract essential oil simultaneously. Food Chemistry, 131, 1399–1405.
[43] Tai J, Cheung S, Wu M, Hasman D (2012). Antiproliferation effect of rosemary (Rosmarinus officinalis) on human ovarian cancer cells in vitro. Phytomedicine, 19, 436–443. 
[44] Thakur P, Kumar S, Malik JA, Berger JD, Nayyar H (2010). Cold stress effects on reproductive development in grain crops: an overview. Environmental and Experimental Botany, 67, 429–443. Crossref
[45] Vaahtera L, Brosché M (2011). More than the sum of its parts-how to achieve a specific transcriptional response to abiotic stress. Plant Science, 180, 421–430. Crossref
[46] Wang H, Gao HY, Zhang ZQ, Wu LJ (2011). Isolation and identification of chemical constituents from Rosmarinus officinalis L. Modern Chinese Medicine, 13(1), 23–25. (in Chinese with English abstract) [王珲, 高慧媛, 张振秋, 吴立军 (2011). 中药迷迭香化学成分的分离与鉴定. 中国现代中药, 13(1), 23–25.] Crossref
[47] Wen Y, Liao FY, Liu ZH (2012). The Effect of water stress on the physiology of Sophora japonica ‘Golden stem’. Chinese Agricultural Science Bulletin, 28(13), 47–52. (in Chinese with English abstract) [文瑛, 廖飞勇, 刘智慧 (2012). 不同水分胁迫对黄枝槐生理特性的影响研究. 中国农学通报, 28(13), 47–52.] Crossref
[48] Yuan GJ, Li PB, Yang H (2012). Anti-MRSA activity of carnosic acid in rosemary. Chinese Journal of Modern Applied Pharmacy, 29, 571–574. (in Chinese with English abstract) [袁干军, 李沛波, 杨慧 (2012). 迷迭香中鼠尾草酸的抗MRSA活性研究. 中国现代应用药学, 29, 571–574.] Crossref
[49] Zhang JX, Kirkham MB (1994). Drought stress induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant and Cell Physiology, 35, 785–791. Crossref
[50] Zhang XF, Kong HY, Li PF, Li JN, Xiong JL, Wang SM, Xiong YC (2011). Recent advances in research on drought induced proteins and the related genes in wheat (Triticum aestivu L.). Acta Ecologica Sinica, 31, 2641–2653. (in Chinese with English abstract) [张小丰, 孔海燕, 李朴芳, 李冀南, 熊俊兰, 王绍明, 熊友才 (2011). 小麦干旱诱导蛋白及相关基因研究进展. 生态学报, 31, 2641– 2653.]
[51] Zhou S, Lin FP, Wang YK, Shen YB, Zhang RM, Gao RF, Gao Y (2012). Effects of mechanical damage of leaves on volatile organic compounds and chlorophyll fluorescence parameters in seedlings of Cinnamomum camphora. Chinese Journal of Plant Ecology, 36, 671–680. (in Chinese with English abstract) [周帅, 林富平, 王玉魁, 沈应柏, 张汝民, 高荣孚, 高岩 (2012). 樟树幼苗机械损伤叶片对挥发性有机化合物及叶绿素荧光参数的影响. 植物生态学报, 36, 671–680.] Crossref
[52] Zuo ZJ, Zhang RM, Wang Y, Hou P, Wen GS, Gao Y (2010). Analysis of main volatile organic compounds and study of aboveground structures in Artemisia frigida. Chinese Journal of Plant Ecology, 34, 462–468. (in Chinese with English abstract) [左照江, 张汝民, 王勇, 侯平, 温国胜, 高岩 (2010). 冷蒿挥发性有机化合物主要成分分析及其地上部分结构研究. 植物生态学报, 34, 462–468.] Crossref
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[2] Jiang Gao-ming. The Impact of Globae Increasing of CO2 on Plants[J]. Chin Bull Bot, 1995, 12(04): 1 -7 .
[3] Zhang Jun Han Bi-wen. Advance in the Study of Histochemical Localization for[J]. Chin Bull Bot, 1995, 12(专辑3): 131 -142 .
[4] Tang Yan-cheng. A Short Guide to the International Code of Botanical Nomenclature V.[J]. Chin Bull Bot, 1984, 2(04): 51 -57 .
[5] Xu Ji. The Protective Protein of Nitrogenase Against Oxygen Damage-Fe-S Protein[J]. Chin Bull Bot, 1986, 4(12): 1 -4 .
[6] . [J]. Chin Bull Bot, 2001, 18(05): 633 .
[7] Huang Zhao-xiang;Zheng Zhen-gui and Zhu Du. Ecological Effect of Taxodium ascendens-Oryza sativa Ecosystem(I) The Growing Characteristic of Taxodium Ascendens in the Ecosystem[J]. Chin Bull Bot, 1996, 13(02): 48 -51 .
[8] GU Rui-Sheng;LIU Qun-Lu;CHEN Xue-Mei and JIANG Xiang-Ning. Comparison and Optimization of the Methods on Protein Extraction and SDS-PAGE in Woody Plants[J]. Chin Bull Bot, 1999, 16(02): 171 -177 .
[9] Jiang Gao-ming. LI-6400 Portable Photosynthesis System: Principle, Function, Basic Operation and Main Problems and Solutions During Measurement[J]. Chin Bull Bot, 1996, 13(增刊): 72 -76 .
[10] Li Ling;Luo Yun-xiu;He Jian-hui and Pan Rui-chi. Promoting the Formation of Adventitious Roots in Cutting of Some Woody Plants by GL Reagent[J]. Chin Bull Bot, 1996, 13(增刊): 63 -65 .