Abstract: A better understanding of the effect of temperature and moisture on soil nitrogen mineralization is important to aid our ability to predict soil N cycling. The objective of this study was to evaluate the effects of temperature (T) and soil water content (θ) on nitrogen mineralization in a deciduous broad-leaved forest. Extracted soil cores were kept in a PVC tube adjusted to different soil water contents (0.12 kg·kg-1, 0.2 kg·kg-1, 0.28 kg·kg-1, 0.35 kg·kg-1 and 0.4 kg·kg-1) and incubated at 5℃, 15℃, 25℃ and 35℃ for 30 days. The inorganic N in the soil extracts before and after incubation was analyzed. The results showed that soil nitrogen net mineralization and net nitrification rates were positively related to T and θ effects (p<0. 001) from 5℃ to 25℃. And that there was a decrease in net mineralization rate and nitrification rate when the temperature was raised above 25℃ and water content was more than 0.20 kg·kg-1. These results suggest an interactive effect of temperature and moisture on mineralization rate and nitrification rate. Two dimensional (T, θ) equations were developed to describe these effects.