Effect of media and light on asymbiotic germination of Malaxis monophyllos seeds

doi:10.3773/j.issn.1005-264x.2010.04.009

Abstract

Abstract:

Aims Extensive collection, environmental deterioration, slow seed germination and low germination percentage have seriously degraded the natural resources of Malaxis monophyllos, resulting in a vulnerable situation for the species. Our objective is to establish an asymbiotic germination system of M. monophyllos seeds to protect and propagate the species.

Methods Seed morphology was investigated by optical microscope and scanning electron microscope. We used Knudson C medium supplemented with 2% sucrose, 3% activated charcoal and 0.8% agar as the basic medium to investigate the effects of α-naphthalene acetic acid (NAA), 6-benzylaminopurine (6-BA), coconut milk, meshed banana, meshed potato and light on seed germination via the orthogonal test.

Important findings Periclinal walls of testa cells were smooth and had no veins, but anticlinal walls were thick and had clear surface patterns. Through the orthogonal test and redundancy analysis, an optimal formula for asymbiotic seed germination was determined: the medium supplemented with 4.5 mg·L^-1 NAA, 10 mg·L^-1 6-BA, 8% meshed banana and 3% meshed potato in the light (12 h light/12 h dark at 20 μmol·m^-2·s^-1 of light intensity). Seed germination was > 90% with this formula.

Key words: asymbiotic germination, Malaxis monophyllos, orthogonal test, redundancy analysis, seed morphology

WANG Rui-Xia, HE Ming-Gao, SONG Song-Quan. Effect of media and light on asymbiotic germination of Malaxis monophyllos seeds[J]. Chin J Plant Ecol, 2010, 34(4): 438-443.

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https://www.plant-ecology.com/EN/Y2010/V34/I4/438

Figures/Tables 6

References 28

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水平 Level	因素 Factor
水平 Level	A, NAA (mg·L^-1)	B, 6-BA (mg·L^-1)	C, 椰乳 Coconut milk (%, v/v)	D, 香蕉泥 Meshed banana (%, w/v)	E, 土豆泥 Meshed potato (%, w/v)
1	0	0	0	0	0
2	0.5	1	4	4	3
3	1.5	3	8	8	6
4	4.5	10	10	10	12

水平 Level	因素 Factor
水平 Level	A, NAA (mg·L^-1)	B, 6-BA (mg·L^-1)	C, 椰乳 Coconut milk (%, v/v)	D, 香蕉泥 Meshed banana (%, w/v)	E, 土豆泥 Meshed potato (%, w/v)
1	0	0	0	0	0
2	0.5	1	4	4	3
3	1.5	3	8	8	6
4	4.5	10	10	10	12

处理Treatment	A	B	C	D	E	光照 Light		黑暗 Darkness
处理Treatment	A	B	C	D	E	萌发率Germination (%)	圆球茎诱导率Protocorm (%)		萌发率 Germination (%)	圆球茎诱导率Protocorm (%)
1	1	1	1	1	1	10.75 ± 1.75^fg	0.00 ± 0.00^d	73.00 ± 2.00^b		7.61 ± 1.28^a
2	1	2	2	2	2	45.00 ± 3.50^c	17.97 ± 2.51^a	52.50 ± 3.50^d		1.34 ± 1.34^bc
3	1	3	3	3	3	40.00 ± 6.00^cd	3.64 ± 0.70b^cd	36.50 ± 1.50^e		0.00 ± 0.00^c
4	1	4	4	4	4	23.00 ± 4.00^ef	0.00 ± 0.00^d	74.00 ± 4.00^b		0.00 ± 0.0^0c
5	2	1	2	3	4	79.00 ± 3.00^b	7.89 ± 0.65^abc	76.50 ± 2.50^ab		0.00 ± 0.00^c
6	2	2	1	4	3	3.50 ± 2.50^g	0.00 ± 0.00^d	81.00 ± 5.50^ab		4.25 ± 0.75^ab
7	2	3	4	1	2	20.50 ± 3.50e^f	0.69 ± 0.69^cd	78.67 ± 1.67^ab		3.28 ± 0.04^bc
8	2	4	3	2	1	40.75 ± 3.75^cd	4.39 ± 1.02^abcd	2.50 ± 1.50^g		0.00 ± 0.00^c
9	3	1	3	4	2	10.75 ± 2.50^g	0.00 ± 0.00^d	2.00 ± 1.00^g		0.00 ± 0.00^c
10	3	2	4	3	1	19.67 ± 3.67ef	16.96 ± 4.46^ab	15.50 ± 4.00^f		0.00 ± 0.00^c
11	3	3	1	2	4	86.25 ± 4.75^ab	7.61 ± 3.93^abc	85.25 ± 2.25^ab		0.00 ± 0.00^c
12	3	4	2	1	3	28.25 ± 2.75^de	4.55 ± 1.33^abcd	2.50 ± 1.00^g		0.00 ± 0.00^c
13	4	1	4	2	3	18.50 ± 2.50^ef	0.00 ± 0.00^d	83.25 ± 3.75^ab		4.47 ± 0.70^ab
14	4	2	3	1	4	36.50 ± 1.00^cd	6.23 ± 2.23^abc	61.50 ± 3.50^c		2.74 ± 1.88^abc
15	4	3	2	4	1	30.25 ± 0.75^de	1.61 ± 1.61^cd	8.75 ± 1.25^fg		0.00 ± 0.00^c
16	4	4	1	3	2	93.25 ± 2.25^a	8.77 ± 5.11^abc	89.50 ± 2.00^a		5.02 ± 0.45^ab

处理Treatment	A	B	C	D	E	光照 Light		黑暗 Darkness
处理Treatment	A	B	C	D	E	萌发率Germination (%)	圆球茎诱导率Protocorm (%)		萌发率 Germination (%)	圆球茎诱导率Protocorm (%)
1	1	1	1	1	1	10.75 ± 1.75^fg	0.00 ± 0.00^d	73.00 ± 2.00^b		7.61 ± 1.28^a
2	1	2	2	2	2	45.00 ± 3.50^c	17.97 ± 2.51^a	52.50 ± 3.50^d		1.34 ± 1.34^bc
3	1	3	3	3	3	40.00 ± 6.00^cd	3.64 ± 0.70b^cd	36.50 ± 1.50^e		0.00 ± 0.00^c
4	1	4	4	4	4	23.00 ± 4.00^ef	0.00 ± 0.00^d	74.00 ± 4.00^b		0.00 ± 0.0^0c
5	2	1	2	3	4	79.00 ± 3.00^b	7.89 ± 0.65^abc	76.50 ± 2.50^ab		0.00 ± 0.00^c
6	2	2	1	4	3	3.50 ± 2.50^g	0.00 ± 0.00^d	81.00 ± 5.50^ab		4.25 ± 0.75^ab
7	2	3	4	1	2	20.50 ± 3.50e^f	0.69 ± 0.69^cd	78.67 ± 1.67^ab		3.28 ± 0.04^bc
8	2	4	3	2	1	40.75 ± 3.75^cd	4.39 ± 1.02^abcd	2.50 ± 1.50^g		0.00 ± 0.00^c
9	3	1	3	4	2	10.75 ± 2.50^g	0.00 ± 0.00^d	2.00 ± 1.00^g		0.00 ± 0.00^c
10	3	2	4	3	1	19.67 ± 3.67ef	16.96 ± 4.46^ab	15.50 ± 4.00^f		0.00 ± 0.00^c
11	3	3	1	2	4	86.25 ± 4.75^ab	7.61 ± 3.93^abc	85.25 ± 2.25^ab		0.00 ± 0.00^c
12	3	4	2	1	3	28.25 ± 2.75^de	4.55 ± 1.33^abcd	2.50 ± 1.00^g		0.00 ± 0.00^c
13	4	1	4	2	3	18.50 ± 2.50^ef	0.00 ± 0.00^d	83.25 ± 3.75^ab		4.47 ± 0.70^ab
14	4	2	3	1	4	36.50 ± 1.00^cd	6.23 ± 2.23^abc	61.50 ± 3.50^c		2.74 ± 1.88^abc
15	4	3	2	4	1	30.25 ± 0.75^de	1.61 ± 1.61^cd	8.75 ± 1.25^fg		0.00 ± 0.00^c
16	4	4	1	3	2	93.25 ± 2.25^a	8.77 ± 5.11^abc	89.50 ± 2.00^a		5.02 ± 0.45^ab