Chin J Plant Ecol ›› 2011, Vol. 35 ›› Issue (2): 176-186.DOI: 10.3724/SP.J.1258.2011.00176
Special Issue: 青藏高原植物生态学:生理生态学
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SHI Sheng-Bo1,2,*(), SHANG Yan-Xia2, ZHU Peng-Jin2, ZHANG De-Gang1
Received:
2010-07-05
Accepted:
2010-11-01
Online:
2011-07-05
Published:
2011-01-21
SHI Sheng-Bo, SHANG Yan-Xia, ZHU Peng-Jin, ZHANG De-Gang. Effects of UV-B exclusion on photosynthetic physiology in alpine plant Saussurea superba[J]. Chin J Plant Ecol, 2011, 35(2): 176-186.
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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00176
amb UV-B | low UV-B | 差异显著性 Significance | |
---|---|---|---|
光合有效辐射 PAR (μmol photon·m-2·s-1) | 1 767 ± 33 | 1 726 ± 43 | 0.398 |
大气相对湿度 Air relative humidity (%) | 65.06 ± 0.23 | 64.88 ± 0.16 | 0.524 |
空气温度 Air temperature (℃) | 24.09 ± 0.06 | 24.03 ± 0.02 | 0.340 |
紫外线-B UV-B (W·m-2) | 4.05 ± 0.10 | 1.74 ± 0.27 | 0 |
紫外线-A UV-A (mW·m -2) | 18.74 ± 0.28 | 15.27 ± 0.25 | 0 |
Table 1 Changes of main environmental factors in UV-B-exclusion experiments in July 18, 2009 (mean ± SE, n = 15; p = 0.05)
amb UV-B | low UV-B | 差异显著性 Significance | |
---|---|---|---|
光合有效辐射 PAR (μmol photon·m-2·s-1) | 1 767 ± 33 | 1 726 ± 43 | 0.398 |
大气相对湿度 Air relative humidity (%) | 65.06 ± 0.23 | 64.88 ± 0.16 | 0.524 |
空气温度 Air temperature (℃) | 24.09 ± 0.06 | 24.03 ± 0.02 | 0.340 |
紫外线-B UV-B (W·m-2) | 4.05 ± 0.10 | 1.74 ± 0.27 | 0 |
紫外线-A UV-A (mW·m -2) | 18.74 ± 0.28 | 15.27 ± 0.25 | 0 |
Fig. 1 Effects of removal of UV-B component from natural sunlight on photosynthesis in Saussurea superba in July 2009. Vertical bar is SE. *, p < 0.05; **, p < 0.01. Ci, intercellular CO2 concentration; Pn, net photosynthetic rate; Gs, stomatal conductance.
Fig. 2 Effects of removal of UV-B components from natural sunlight on 3 min dark adapted quantum efficiency of PSII photochemistry (F(v)/F(m)) in Saussurea superba in August 2008. Data numbers were from 4 to 22 during the 16 days of continue measurement due to the influence of weather condition. Vertical bar is SE. **, p < 0.01.
Fig. 3 Effects of removal of UV-B components from natural sunlight on light adapted photochemistry efficiency of PSII in Saussurea superba in August 2008. Fv′/Fm′, photochemical efficiency of PSII in the light; ΦPSII, actual photochemical efficiency of PSII. Vertical bar is SE. *, p < 0.05.
Fig. 4 Effects of removal of UV-B components from natural sunlight on photochemical and non-photochemical quenching coefficient in Saussurea superba in August 2008. NPQ, nonphotochemical quenching; qp, the coefficient of photochemical quenching. Vertical bar is SE. *, p < 0.05.
amb UV-B | low UV-B | 差异显著性 Significant | ||
---|---|---|---|---|
叶绿素a Chl a | mg·g-1 (FW) | 1.068 1 ± 0.029 1 | 1.117 9 ± 0.094 8 | 0.645 |
μg·cm-2 (leaf area) | 0.034 4 ± 0.000 8 | 0.038 8 ± 0.003 6 | 0.304 | |
叶绿素b Chl b | mg·g-1 (FW) | 0.324 7 ± 0.011 6 | 0.337 3 ± 0.024 1 | 0.655 |
μg·cm-2 (leaf area) | 0.010 5 ± 0.000 4 | 0.011 7 ± 0.000 9 | 0.276 | |
总叶绿素 Chl a+b | mg·g-1 (FW) | 1.392 9 ± 0.040 1 | 1.455 2 ± 0.118 7 | 0.637 |
μg·cm-2 (leaf area) | 0.044 8 ± 0.001 2 | 0.050 5 ± 0.004 5 | 0.297 | |
类胡萝卜素 Car | mg·g-1 (FW) | 0.312 4 ± 0.011 9 | 0.335 3 ± 0.039 5 | 0.599 |
μg·cm-2 (leaf area) | 0.010 1 ± 0.000 5 | 0.011 7 ± 0.001 5 | 0.363 | |
Car/Chl a+b | 0.224 5 ± 0.008 3 | 0.228 4 ± 0.010 1 | 0.784 | |
Chl a/b | 3.292 3 ± 0.046 5 | 3.305 1 ± 0.052 8 | 0.861 |
Table 2 Effects of removal of UV-B component from natural sunlight on photosynthetic pigment contents in Saussurea superba in August 2008 (mean ± SE, n = 5; p = 0.05)
amb UV-B | low UV-B | 差异显著性 Significant | ||
---|---|---|---|---|
叶绿素a Chl a | mg·g-1 (FW) | 1.068 1 ± 0.029 1 | 1.117 9 ± 0.094 8 | 0.645 |
μg·cm-2 (leaf area) | 0.034 4 ± 0.000 8 | 0.038 8 ± 0.003 6 | 0.304 | |
叶绿素b Chl b | mg·g-1 (FW) | 0.324 7 ± 0.011 6 | 0.337 3 ± 0.024 1 | 0.655 |
μg·cm-2 (leaf area) | 0.010 5 ± 0.000 4 | 0.011 7 ± 0.000 9 | 0.276 | |
总叶绿素 Chl a+b | mg·g-1 (FW) | 1.392 9 ± 0.040 1 | 1.455 2 ± 0.118 7 | 0.637 |
μg·cm-2 (leaf area) | 0.044 8 ± 0.001 2 | 0.050 5 ± 0.004 5 | 0.297 | |
类胡萝卜素 Car | mg·g-1 (FW) | 0.312 4 ± 0.011 9 | 0.335 3 ± 0.039 5 | 0.599 |
μg·cm-2 (leaf area) | 0.010 1 ± 0.000 5 | 0.011 7 ± 0.001 5 | 0.363 | |
Car/Chl a+b | 0.224 5 ± 0.008 3 | 0.228 4 ± 0.010 1 | 0.784 | |
Chl a/b | 3.292 3 ± 0.046 5 | 3.305 1 ± 0.052 8 | 0.861 |
[1] | Baker NR (1996). Photoinhibition of photosynthesis. In: Jennings RC, Zucchelli G, Ghetti F, Colombetti G eds. Light as Energy Source and Information Carrier in Plant Physiology. Plenum Press, New York. 89-96. |
[2] |
Baker NR (2008). Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Review of Plant Biology, 59, 89-113.
DOI URL PMID |
[3] | Beggs CG, Wellmann E (1994). Photocontrol of flavonoid biosynthesis. In: Kendrick RE, Kronenberg GHM eds. Photomorphogenesis in Plants. Volume 2. Kluwer Academic 733-750. |
[4] |
Bilger W, Björkman O (1990). Role of the xanthophyll cycle photoprotection elucidated by measurements of light- induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynthesis Research, 25, 173-185.
DOI URL PMID |
[5] | Björn LO (1999). Ultraviolet-B radiation, the ozone layer and ozonedepletion. In: Rozema J ed. Stratospheric Ozone Depletion: the Effects of Enhanced UV-B Radiation on Terrestrial Ecosystems. Backhuys Publishers, Leiden, The Netherlands. 21-27. |
[6] | Caldwell MM, Flint SD (1994). Stratospheric ozone reduction, solar UV-B radiation and terrestrial ecosystem. Climate Change, 28, 375-394. |
[7] | Costa H, Gallego SM, Tomaro ML (2002). Effects of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Science, 162, 939-945. |
[8] | Day TA, Neale PJ (2002). Effects of UV-B radiation on terrestrial and aquatic primary producers. Annual Review of Ecological System, 33, 371-396. |
[9] | Fedina I, Georgieva K, Velitchkova M, Grigorova I (2006). Effect of pretreatment of barley seedlings with different salts on the level of UV-B induced and UV-B absorbing compounds. Environmental and Experimental Botany, 56, 225-230. |
[10] |
Fiscus EL, Booker FL (1995). Is increased UV-B a threat to crop photosynthesis and productivity? Photosynthesis Research, 43, 81-92.
DOI URL PMID |
[11] | Fiscus EL, Philbeck R, Britt AB, Booker FL (1999). Growth of Arabidopsis flavonoid mutants under solar radiation and UV filter. Environmental and Experimental Botany, 41, 231-245. |
[12] | Flint SD, Ryel RJ, Caldwell MM (2003). Ecosystem UV-B experiments in terrestrial communities: a review of recent findings and methodologies. Agricultural and Forest Meteorology, 120, 177-189. |
[13] |
Galvez-Valdivieso G, Fryer MJ, Lawson T, Slattery K, Truman W, Smimoff N, Asami T, Davies WJ, Jones AM, Baker NR, Mullineaux PM (2009). The high light response in Arabidopsis involves ABA signaling between vascular and bundle sheath cells. The Plant Cell, 21, 2143-2162.
DOI URL PMID |
[14] | Gassi-Lit M, Whitecross MJ, Nayudu M, Tanner GJ (1997). UV-B irradiation induces differential leaf damage, ultra-structural changes and accumulation of specific phenolic compounds in rice cultivars. Australian Journal of Plant Physiology, 24, 261-274. |
[15] | Genty B, Briantais JM, Baker NR (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta, 990, 87-92. |
[16] | Jansen MAK, Gaba V, Greenberg BM (1998). Higher plants and UV-B radiation: balancing damage, repair and acclimation. Trends in Plant Science, 3, 131-135. |
[17] | Lau TSL, Eno E, Goldstein G, Smith C, Christopher DA (2006). Ambient levels of UV-B in Hawaii combined with nutrient deficiency decrease photosynthesis in near- isogenic maize lines varying in leaf flavonoids: Flavonoids decrease photoinhibition in plants exposed to UV-B. Photosynthetica, 44, 394-403. |
[18] | Lizana XC, Hess S, Calderini DF (2009). Crop phenology modifies wheat responses to increased UV-B radiation. Agricultural and Forest Meteorology, 149, 1964-1974. |
[19] | Madronich S, McKenzie RL, Caldwell MM, Björn LO (1995). Changes in ultraviolet radiation reaching the earth’s surface. Ambio, 24, 143-152. |
[20] | Middleton EM, Teramura AH (1993). The role of flavonol glycoside and carotenoids in protecting soybean from ultraviolet-B damage. Plant Physiology, 103, 475-480. |
[21] | Oxborough K, Baker NR (1997). Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components-calculation of qP and Fv′/Fm′ without measuring F0′. Photosynthesis Research, 54, 135-142. |
[22] | Paul ND, Gwynn-Jones D (2003). Ecological roles of solar UV radiation: towards an integrated approach. Trends in Ecology and Evolution, 18, 48-55. |
[23] | Petropoulou Y, Georgiou O, Psaras GK, Manetas Y (2001). The growth, flower properties and demography of Anthemis arvensis exposed to enhanced UV-B radiation. Plant Ecology, 154, 59-64. |
[24] | Pinto ME, Casati P, Hus TP, Ku MSB, Edwards GE (1999). Effects of UV-B radiation on growth, photosynthesis, UV-B-absorbing compounds and NADP-malic enzyme in bean ( Phaseolus vulgaris L.) grown under different nitrogen condition. Journal of Photochemistry and Photobiology B: Biology, 48, 200-209. |
[25] | Quick WP, Stitt M (1989). An examination of factors contributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves. Biochimica et Biophysica Acta, 977, 287-296. |
[26] | Rozema J, Björn LO, Bornman JF, Gaberščik A, Häder DP, Trošt T, Germ M, Klisch M, Gröniger A, Sinha RP, Lebert M, He YY, Buffoni-Hall R, de Bakker NVJ, van de Staaij J, Meijkamp BB (2002). The role of UV-B radiation in aquatic and terrestrial ecosystem—an experimental and functional analysis of evolution of UV-absorbing compounds. Journal of Photochemistry and Photobiology B: Biology, 66, 2-12. |
[27] |
Shi SB, Zhu WY, Li HM, Zhou DW, Han F, Zhao XQ, Tang YH (2004). Photosynthesis of Saussurea superba and Gentiana straminea is not reduced after long-term enhancement of UV-B radiation. Environmental and Experimental Botany, 51, 75-83.
DOI URL |
[28] |
Sicora C, Máté Z, Vass I (2003). The interaction of visible and UV-B light during photodamage and repair of photosystem II. Photosynthesis Research, 75, 127-137.
DOI URL |
[29] | Sicora C, Szilárd A, Sass L, Turcsányi E, Máté Z, Vass I (2006). UV-B and UV-A radiation effects on photosynthesis at the molecular level. In: Ghetti F, Checcucci G, Bornman JF eds. Environmental UV Radiation: Impact on Ecosystem and Human Health and Predictive Model. Springer, The Netherlands. 121-135. |
[30] |
Šprtová M, Špunda V, Kalina J, Marek MV (2003). Photosynthetic UV-B response of beach ( Fagus sylvatica L.) saplings. Photosynthetic, 41, 533-543.
DOI URL |
[31] | Strid A, Porra RJ (1992). Alterations in pigments content in leaves of Pisum sativum after expose to supplementary UV-B. Plant Cell Physiology, 33, 1015-1023. |
[32] | van Rensen JJ, Vredenberg WJ, Rodrigues GC (2007). Time sequence damage to the acceptor and donor sides of photosystem II by UV-B radiation as evaluated by chlorophyll a fluorescence. Photosynthesis Research, 94, 219-297. |
[33] | Wang GH, Hao ZJ, Anken RH, Lu JY, Liu YD (2010). Effects of UV-B radiation on photosynthesis activity of Wolffia arrhiza as probed by chlorophyll fluorescence transients. Advances in Space Research, 45, 839-845 |
[34] | Xu DQ (许大全) (2002). Photosynthetic Efficiency (光合作用效率). Shanghai Scientific and Technical Publishers, Shanghai. (in Chinese) |
[35] | Zhang SR (张守仁) (1999). A discussion on chlorophyll fluorescence kinetics parameters and their significance. Chinese Bulletin of Botany (植物学通报), 16, 444-448. (in Chinese with English abstract) |
[36] | Zhu GL (朱广廉), Zhong HW (钟诲文), Zhang AQ (张爱琴) (1990). The Plant Physiological Experiment (植物生理学实验). Beijing University Press, Beijing. 51-54. (in Chinese) |
[37] | Ziska LH, Termura AH, Sullivan JH (1992). Physiological sensitivity of plants along an elevational gradient to UV-B radiation. American Journal of Botany, 79, 863-871. |
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