Application of Lake-model based indices from chlorophyll fluorescence on sugarcane seedling drought resistance study

doi:10.17521/cjpe.2015.0039

Abstract

Abstract: Aims

Spring drought greatly limits the sugarcane (Saccharum officinarum) seedling growth in the southwest China. A major objective of this study is to investigate the change of the energy allocation in photosystem II (PSII) resulted from the stomatal and non-stomatal limit of photosynthesis under the drought stress condition. The study results can be used for drought resistant breeding and rapid drought stress diagnosis.

Methods

Four levels (40%, 25%, 10% and 8%) of soil volumetric water content (VWC) have been chosen from natural drought treatments. The chlorophyll fluorescence parameters based on Lake-model were measured and analyzed with various levels of photosynthetically active radiation (PAR).

Important findings

Results of the study in the enhanced drought stress for two tested cultivars (‘ROC22’ and ‘ROC16’) showed 1) decreased numbers in the maximum quantum use efficiency (F_v/F_m), the relative electron transport rate (rETR), the quantum efficiency of PSII (Φ_II) and the photochemical quenching (q_L) and 2) increased numbers in the down-regulated energy dissipation (Φ_NPQ) and the non-light induced energy dissipation (Φ_NO). There was no significant difference between the parameter q_L of ‘ROC16’ and ‘ROC22’, except for the 8% level where the q_L showed a sharp decline. The results indicated that the PSII of ‘ROC16’ remained a relatively high open fraction during the mild drought stress, but suffered severe damage in serious drought stress. The rising range of Φ_NPQ for ‘ROC22’ were higher and more sensitive to drought stress than that of ‘ROC16’, which revealed a strong drought resistance resulted from strong photo-protective mechanism in‘ROC22’. The Φ_NO was more sensitive to drought stress than F_v/F_m especially for non drought resistant variety, and remained high stability under different PAR. Therefore, Φ_NO could be properly used as an indicator in drought stress diagnosis and resistance evaluation. Photo inhibition could be the initial factor leading to PSII damage and its signal had been amplified under high PAR related to photo inhibition since the appeared peak or decline of rETR related to increased PAR and its no significance at each water level and low PAR during drought stress.

Key words: chlorophyll fluorescence, drought stress, Lake-model, sugarcane, drought resistance evaluation

AN Dong-Sheng,CAO Juan,HUANG Xiao-Hua,ZHOU Juan,DOU Mei-An. Application of Lake-model based indices from chlorophyll fluorescence on sugarcane seedling drought resistance study[J]. Chin J Plan Ecolo, 2015, 39(4): 398-406.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2015.0039

https://www.plant-ecology.com/EN/Y2015/V39/I4/398

Figures/Tables 4

Table 1 Soil volumetric water content (VWC) of the two cultivars of Saccharum officinarum after drought treatment

处理天数 Days after treatment	品种 Cultivars
	‘ROC22’		‘ROC16’
	VWC (%)	标准偏差 Standard deviation (%)	VWC (%)	标准偏差 Standard deviation (%)
1	74.9	3.6	74.4	3.6
2	64.4	3.9	59.2	4.6
3	52.4	3.6	40.1	4.0
4	40.0	2.6	24.7	3.3
5	25.9	3.5	10.9	1.4
6	17.7	2.1	8.6	1.0
7	10.2	1.7	5.9	1.4
8	7.6	1.5	3.0	0.0

Fig. 1 Effects of drought stress on photosystem II activities under different light intensity (mean ± SD). rETR, relative electron transport rate; ΦII, quantum efficiency of photosystem II; PAR, photosynthetically active radiation; VWC, volumetric water content.

Fig. 2 Effects of drought stress on chlorophyll fluorescence quenching under different light intensity (mean ± SD). qL, photochemical quenching based on Lake-model; ΦNPQ, down-regulated energy dissipation; ΦNPQ, non-light induced energy dissipation; PAR, photo-synthetically active radiation; VWC, volumetric water content.

Fig. 3 Effects of drought stress on the maximum quantum use efficiency (Fv/Fm) and the non-light induced energy dissipation (ΦNO) (mean ± SD); VWC, volumetric water content.

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