Chin J Plan Ecolo ›› 2016, Vol. 40 ›› Issue (6): 585-593.DOI: 10.17521/cjpe.2015.0292

• Research Articles • Previous Articles     Next Articles

Effects of long-term flooding on respiratory metabolism of Taxodium ‘Zhongshansha 118’ seedlings

Yan-Ting ZHANG1, Jian-Jun ZHANG1,*(), Jian-Xiu WANG2, Xiao-Hong WU3, Bao-Qiang CHEN1, Peng-Fei LI1, Zhi-Zhen WANG1   

  1. 1School of Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China

    2Forest Bureau in Kai County, Kai County, Chongqing 405400, China

    3Chongqing Hejia Spice Plant Limited Company, Chongqing 404000, China
  • Received:2015-08-11 Accepted:2016-03-26 Online:2016-06-30 Published:2016-06-15
  • Contact: Jian-Jun ZHANG

Abstract:

Aims Taxodium ‘Zhongshansha’ had a strong submergence tolerance, but the biological mechanism is not clearly defined. The respiratory metabolism for the tolerance of Taxodium ‘Zhongshansha 118’ ((Taxodium distichum × Taxodium mucronatum) × Taxodium mucronatum) to long-term flooding was investigated through the measuring and analyzing the changes in root starch and soluble sugar as well as the contents of anaerobic respiration enzymes, including lactic dehydrogenase (LDH), ethanol dehydrogenase (ADH) and pyruvate decarboxylase (PDC)), in the ‘Zhongshansha 118’ seedling. The biomass and activities of the seedling roots were also measured and analyzed.Methods 24 1-year Taxodium ‘Zhongshansha 118’ seedlings were randomly and equally divided into four groups and each group experienced one of four different levels of flooding (i.e., no flooding, waterlogging, partial submergence, deep submergence) from August 8 to November 8, 2014.Important findings The results showed that the contents of the anaerobic respiration enzymes in the seedling roots and leaves are increasing with the levels of severity of flooding, which indicated the roots and leaves adapt to long-term flooding by reinforcing their anaerobic respiration and activities of ADH that were higher than LDH for roots and leaves used in alcoholic fermentation mainly. The activities of anaerobic respiration enzymes in leaves were higher than that in roots, while leaves were more sensitive to flooding. The starch and soluble sugar in roots and leaves had similar trend with anaerobic respiration enzymes. However, roots starch was higher than leaves where soluble sugar was lower than roots. The higher content of starch were the important reason of strong submergence tolerance, and we consider the strategy to fit into long-term flooding is patience type. The paper demonstrate Taxodium ‘Zhongshansha 118’ have the physiological and the morphological properties to adapt to long-term flooding, including aerial root and basal part of stem expanded and the outer wall of the root lignified to protect the roots from flooding.

Key words: flooding stress, anaerobic respiration enzymes, soluble sugar, flooding tolerance, Taxodium ‘Zhongshansha’