Chin J Plant Ecol ›› 2014, Vol. 38 ›› Issue (9): 959-969.DOI: 10.3724/SP.J.1258.2014.00090
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PENG Dong-Hai1,*(),YANG Jian-Bo1,LI Jian1,XING Yong-Xiu1,2,QIN Liu-Dong1,YANG Li-Tao1,2,**(),LI Yang-Rui1,2,**()
Received:
2014-01-07
Accepted:
2014-04-14
Online:
2014-01-07
Published:
2014-09-22
Contact:
PENG Dong-Hai,YANG Li-Tao,LI Yang-Rui
PENG Dong-Hai,YANG Jian-Bo,LI Jian,XING Yong-Xiu,QIN Liu-Dong,YANG Li-Tao,LI Yang-Rui. Effects of intercropping with soybean on bacterial and nitrogen-fixing bacterial diversity in the rhizosphere of sugarcane[J]. Chin J Plant Ecol, 2014, 38(9): 959-969.
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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2014.00090
Fig. 1 Extraction of total soil DNA. 1, control, stage 1; 2, rhizospheric soil of soybean, stage 1; 3, control, stage 2; 4, rhizospheric soil of soybean, stage 2; 5, rhizospheric soil of ‘B8’, monoculture, stage 1; 6, rhizospheric soil of ‘B8’, intercropping, stage 1; 7, rhizospheric soil of ‘B8’, monoculture, stage 2; 8, rhizospheric soil of ‘B8’, intercropping, stage 2; 9, rhizospheric soil of ‘B8’, monoculture, stage 3; 10, rhizospheric soil of ‘B8’, intercropping, stage 3; 11, rhizospheric soil of ‘ROC22’, monoculture, stage 1; 12, rhizospheric soil of ‘ROC22’, intercropping, stage 1; 13, rhizospheric soil of ‘ROC22’, monoculture, stage 2; 14, rhizospheric soil of ‘ROC22’, intercropping, stage 2; 15, rhizospheric soil of ‘ROC22’, monoculture, stage 3; 16, rhizospheric soil of ‘ROC22’, intercropping, stage 3; 17, rhizospheric soil of ‘GT21’, monoculture, stage 1; 18, rhizospheric soil of ‘GT21’, intercropping, stage 1; 19, rhizospheric soil of ‘GT21’, monoculture, stage 2; 20, rhizospheric soil of ‘GT21’, intercropping, stage 2; 21, rhizospheric soil of ‘GT21’, monoculture, stage 3; 22, rhizospheric soil of ‘GT21’, intercropping, stage 3. M, DNA mark (Mark II, Tiangen). stage 1, the highest at soybean booming stage (22 May); stage 2, soybean maturity (2 July); stage 3, sugarcane elongation stage (15 Sept.).
Fig. 3 The products of nifH gene in second round of PCR amplification. Notes are the same as in Fig. 1. “+” and “-” are positive and negative controls, respectively.
Fig. 5 Cluster analysis on denaturing gradient gel electrophoresis (DGGE) bands of bacterial 16S rRNA gene in different soil samples. 1-22 are the same as in Fig. 1.
Fig. 6 Diversity of bacterial communities in different soil samples (mean ± SE). Different letters represent signi?cant differences among treatmens (p < 0.05).
Fig. 7 Denaturing gradient gel electrophoresis bands of nitrogen-fixing bacterial nifH gene in different soil samples. 1-22 are the same as in Fig. 1.
Fig. 8 Cluster analysis on denaturing gradient gel electrophoresis bands of nitrogen-fixing bacterial nifH gene in different soil samples. 1-22 are the same as in Fig. 1.
Fig. 9 Diversity of nitrogen-fixing bacteria in different soil samples (mean ± SE). Different letters represent signi?cant differences among treatments (p < 0.05).
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