Chin J Plant Ecol ›› 2010, Vol. 34 ›› Issue (10): 1213-1219.DOI: 10.3773/j.issn.1005-264x.2010.10.010
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YAN Yong-Qing*(), LIU Xing-Liang, WANG Kun, FAN Jin-Ping, SHI Xi-Chan
Received:
2009-10-26
Accepted:
2010-06-12
Online:
2010-10-26
Published:
2010-10-31
Contact:
YAN Yong-Qing
YAN Yong-Qing, LIU Xing-Liang, WANG Kun, FAN Jin-Ping, SHI Xi-Chan. Effect of complex saline-alkali stress on physiological parameters of Nitratia tangutorum[J]. Chin J Plant Ecol, 2010, 34(10): 1213-1219.
处理组 Treatment | 盐分组成及摩尔比 Salt composition and molar ratio | 各盐浓度(mmol·L-1)处理组对应的pH值 pH values corresponding to various salinity treatments (mmol·L-1) | |||||||
---|---|---|---|---|---|---|---|---|---|
NaCl | Na2SO4 | NaHCO3 | Na2CO3 | 0 | 100 | 200 | 300 | 400 | |
CK | 0 | 0 | 0 | 0 | 6.87 | - | - | - | - |
A | 1 | 1 | 0 | 0 | - | 7.39 | 7.47 | 7.54 | 7.73 |
B | 1 | 0 | 1 | 0 | - | 8.13 | 8.24 | 8.31 | 8.46 |
C | 1 | 1 | 1 | 1 | - | 9.47 | 9.56 | 9.87 | 9.98 |
D | 0 | 1 | 0 | 1 | - | 10.42 | 10.57 | 10.62 | 10.75 |
E | 0 | 0 | 1 | 1 | - | 11.18 | 11.35 | 11.47 | 11.73 |
Table 1 Salt composition, molar ratio and corresponding pH of solutions of each treament
处理组 Treatment | 盐分组成及摩尔比 Salt composition and molar ratio | 各盐浓度(mmol·L-1)处理组对应的pH值 pH values corresponding to various salinity treatments (mmol·L-1) | |||||||
---|---|---|---|---|---|---|---|---|---|
NaCl | Na2SO4 | NaHCO3 | Na2CO3 | 0 | 100 | 200 | 300 | 400 | |
CK | 0 | 0 | 0 | 0 | 6.87 | - | - | - | - |
A | 1 | 1 | 0 | 0 | - | 7.39 | 7.47 | 7.54 | 7.73 |
B | 1 | 0 | 1 | 0 | - | 8.13 | 8.24 | 8.31 | 8.46 |
C | 1 | 1 | 1 | 1 | - | 9.47 | 9.56 | 9.87 | 9.98 |
D | 0 | 1 | 0 | 1 | - | 10.42 | 10.57 | 10.62 | 10.75 |
E | 0 | 0 | 1 | 1 | - | 11.18 | 11.35 | 11.47 | 11.73 |
Fig. 1 Effect of various complex saline-alkali stress on electrolyte leakage in leaves of Nitratia tangutorum. As the intraclass changes in pH, significantly less than that between groups, so analysis of the data to various concentrations of the same group as the pH value of the average of seven combinations of pH. CK, A, B, C, D, E, see Table 1.
胁变指标 Strain index | 盐浓度 Salinity | pH值 pH value | 盐浓度× pH Salinity× pH | |||||
---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | |||
电解质外渗率 Electrolyte leakage | 288.063 | 0.000** | 64.538 | 0.000** | 14.267 | 0.000** | ||
丙二醛含量 MDA content | 4.071 | 0.000** | 3.821 | 0.070** | 0.796 | 0.053* | ||
SOD活性 SOD activity | 73.532 | 0.000** | 4.862 | 0.023* | 2.908 | 0.318 NS | ||
POD活性 POD activity | 426.774 | 0.002** | 88.905 | 0.000** | 17.193 | 0.000** | ||
CAT活性 CAT activity | 289.215 | 0.003** | 17.339 | 0.000** | 6.313 | 0.000** |
Table 2 Effect of salinity, pH and their interaction effects on different strain indexes of Nitratia tangutorum seedlings
胁变指标 Strain index | 盐浓度 Salinity | pH值 pH value | 盐浓度× pH Salinity× pH | |||||
---|---|---|---|---|---|---|---|---|
F | p | F | p | F | p | |||
电解质外渗率 Electrolyte leakage | 288.063 | 0.000** | 64.538 | 0.000** | 14.267 | 0.000** | ||
丙二醛含量 MDA content | 4.071 | 0.000** | 3.821 | 0.070** | 0.796 | 0.053* | ||
SOD活性 SOD activity | 73.532 | 0.000** | 4.862 | 0.023* | 2.908 | 0.318 NS | ||
POD活性 POD activity | 426.774 | 0.002** | 88.905 | 0.000** | 17.193 | 0.000** | ||
CAT活性 CAT activity | 289.215 | 0.003** | 17.339 | 0.000** | 6.313 | 0.000** |
胁变指标 Strain index | pH | 盐浓度(salinity) | CO32- | HCO3- | 模拟方程 Best-fitting equation | ||||
---|---|---|---|---|---|---|---|---|---|
t | p | t | p | t | p | t | p | ||
电解质外渗率 Electrolyte leakage rate | 5.502 | 0.000** | 7.133 | 0.000** | -2.827 | 0.012* | - | - | Y = 6.996X1 + 0.069X2 + 0.793 - 0.084X3 - 3.67 R2 = 0.934 |
丙二醛含量 MDA content | 5.800 | 0.000** | 7.969 | 0.000** | - | - | - | - | Y = 0.298X1 + 0.005X2 - 1.090 R2 = 0.930 |
SOD活性 SOD activity | -1.628 | 0.022* | 4.611 | 0.000** | - | - | - | - | Y = -7.768X1 + 0.284X2 + 220.670 R2 = 0.855 |
POD活性 POD activity | 3.541 | 0.003** | 3.250 | 0.005** | -3.102 | 0.007** | - | - | Y = 980.800X1 + 6.839X2 - 20.171X3 - 4078.915 R2 = 0.846 |
CAT活性 POD activity | 4.433 | 0.000** | 5.493 | 0.000** | -2.142 | 0.048* | - | - | Y = 9.875X1 + 0.093X2 - 0.112X3 - 49.073 R2 = 0.920 |
Table 3 Partial regression coefficient and test of each variable
胁变指标 Strain index | pH | 盐浓度(salinity) | CO32- | HCO3- | 模拟方程 Best-fitting equation | ||||
---|---|---|---|---|---|---|---|---|---|
t | p | t | p | t | p | t | p | ||
电解质外渗率 Electrolyte leakage rate | 5.502 | 0.000** | 7.133 | 0.000** | -2.827 | 0.012* | - | - | Y = 6.996X1 + 0.069X2 + 0.793 - 0.084X3 - 3.67 R2 = 0.934 |
丙二醛含量 MDA content | 5.800 | 0.000** | 7.969 | 0.000** | - | - | - | - | Y = 0.298X1 + 0.005X2 - 1.090 R2 = 0.930 |
SOD活性 SOD activity | -1.628 | 0.022* | 4.611 | 0.000** | - | - | - | - | Y = -7.768X1 + 0.284X2 + 220.670 R2 = 0.855 |
POD活性 POD activity | 3.541 | 0.003** | 3.250 | 0.005** | -3.102 | 0.007** | - | - | Y = 980.800X1 + 6.839X2 - 20.171X3 - 4078.915 R2 = 0.846 |
CAT活性 POD activity | 4.433 | 0.000** | 5.493 | 0.000** | -2.142 | 0.048* | - | - | Y = 9.875X1 + 0.093X2 - 0.112X3 - 49.073 R2 = 0.920 |
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