Abstract: Scientific planning and rational utilization of land are considered as the most important measure to conserve the existing biodiversity because the loss of biodiversity is mainly caused by land exploitation. Benefiting from diverse climatic conditions, Xishuangbanna is one of the richest area in biodiversity in China. However, due to the increasing of land exploitation ,the biodiversity in this area has been reduced with a considerable speed. The impacts of 8 types of land use on plant biodiversity were analyzed . The order of the 8 types of land use by their intensity of impact on biodiversity was as follows. Monoculture of rubber plantation > Rubber + Tea > Rubber +Pineapple>Home garden>Temple garden>8-year abounded lands after shifting cultivation>Forest+Chinese cardamom>Collective Forest>Nature reserve.