新疆沙冬青抗冻蛋白的提取分离及其热滞活性测定

Plant Diversity ›› 2007, Vol. 29 ›› Issue (02): 251-255.

新疆沙冬青抗冻蛋白的提取分离及其热滞活性测定

尉姗姗, 尹林克, 牟书勇, 鲁春芳, 赵峰侠

中国科学院新疆生态与地理研究所, 新疆乌鲁木齐 830011

收稿日期:2006-06-20 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 尹林克

Extraction, Separation and Measuration of the Thermal Hysteresis Activity of Antifreeze Proteins from Ammopiptanthus nanus (Leguminosae)

YU Shan-Shan , YIN Lin-Ke* * , MU Shu-Yong, LU Chun-Fang , ZHAO Feng-Xia

Xinjiang Institute of Ecology and Geography , Chinese Academy of Sciences, Urumqi 830011 , China

Received:2006-06-20 Online:2007-04-25 Published:2007-04-25
Contact: YIN Lin-Ke

摘要/Abstract

摘要： 抗冻蛋白被发现于低温环境下生存的生物体中, 它能够吸附在冰晶表面并改变冰晶的生长形态, 对于增加植物的抗低温胁迫能力有重要作用。用纤维素DE-52 离子柱层析提取分离出了冬季新疆沙冬青( Ammopiptanthus nanus) 叶片中的抗冻蛋白(AFPs )。差示扫描量热法(DSC) 测定结果表明, 当蛋白浓度为20 mg/ml 时, 抗冻蛋白的热滞活性( THA) 为0. 46℃。经SDS-PAGE 电泳分析, 此抗冻蛋白的分子量为119 . 24 kDa。

关键词: 新疆沙冬青, 抗冻蛋白, 差示扫描量热法, 热滞活性FONT

Abstract: Antifreeze proteins (AFPs) , because of their ability of adsorbing onto the surface of ice crystals and modifying their growth , have been found in many different organisms that are exposed to chilling and freezing temperatures . It is very important to increase the resisting and coercing ability at low temperature . DE-52 ion- exchange cellulose was used to extract and separate the antifreeze proteins from Ammopiptanthus nanus leaves. Differential scanning calorimetry (DSC) was used to measure the thermal hysteresis activity (THA) . The results reveal that when the concentration of the protein is 20mg/ml , the THA of the AFPs is 0 . 46℃ . Its molecular weight is about 119. 24 kDa by sodium dodecyl sulfate polyacrylam ide gel electrophoresis (SDS-PAGE) .

Key words: Ammopiptanthus nanus

尉姗姗, 尹林克, 牟书勇, 鲁春芳, 赵峰侠 . 新疆沙冬青抗冻蛋白的提取分离及其热滞活性测定[J]. Plant Diversity, 2007, 29(02): 251-255.

YU Shan-Shan , YIN Lin-Ke* * , MU Shu-Yong, LU Chun-Fang , ZHAO Feng-Xia. Extraction, Separation and Measuration of the Thermal Hysteresis Activity of Antifreeze Proteins from Ammopiptanthus nanus (Leguminosae)[J]. Plant Diversity, 2007, 29(02): 251-255.