萱草花粉类动力蛋白生物物理学及药理学性质

Plant Diversity ›› 2007, Vol. 29 ›› Issue (02): 247-250.

萱草花粉类动力蛋白生物物理学及药理学性质

廖俊杰^{1 , 2} , 吴英杰³, 阎隆飞¹

1 中国农业大学生物学院植物生理生化国家开放重点实验室, 北京 100094; 2 广东轻工职业技术学院食品与生物工程系, 广东广州 510300 ; 3 Department of Medicine , Mount Sinai School of Medicine, New York , NY 10029

收稿日期:2006-05-11 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 吴英杰

Biophysical and Pharmacological Characterization of a Dynamin-like Protein from Day-lily ( Hemerocallis fulva , Liliaceae) Pollens

LIAO Jun-Jie^{1 , 2} , WU Ying-Jie³ ^{* *} , YAN Lung-Fei¹

1 College of Biological Sciences, China Agricultural University , State Key Labratory of Plant Physiology and Biochemistry , Beijing 100094 , China; 2 Department of Food and Bio- technology , Guangdong Light Industry Technologic College, Guangzhou 510300 , China ; 3 Department of Medicine, Mount Sinai School of Medicine , New York , NY 10029 , America

Received:2006-05-11 Online:2007-04-25 Published:2007-04-25
Contact: WU Ying-Jie

摘要/Abstract

摘要： 利用FPLC 技术从萱草花粉中鉴定并纯化了动力蛋白, 研究了它的酶学性质及部分生物化学性质。结果如下: 纯化的类动力蛋白分子量为100 kD, 等电点pI = 6. 15 和6 .80。在280 nm 波长激发下, 最大的荧光发射波长是346 nm。荧光光谱分析结合紫外吸收光谱及导数光谱分析推断它含有色氨酸和酪氨酸残基。药理学性质研究表明巯基可能在酶的活性中心起重要用。

关键词: 类动力蛋白, 分子马达, 微管, 花粉FONT

Abstract:

A novel motor protein , dynamin- like protein from day lily, was purified and identified by FPLC . Here we report its biochemical characterization . The molecular weight of the dynamin-like protein is 100 kD on SDS-PAGE. Isoelectric points are about 6. 15 and 6 .80 . The fluorescence emission wave length of dynamin-like protein is 346 nm by excitation at 280 nm . Through fluorescence spectra analysis, ultraviolet absorption spectrum and derivative spectrum, we conclude that it contains tryptophan and tyrosine residues . Pharmacological study indicates that mercapto may play an important role in
enzyme activity of dynamin-like protein .

Key words: Dynamin-like protein

廖俊杰1 , 2 , 吴英杰3 , 阎隆飞1 . 萱草花粉类动力蛋白生物物理学及药理学性质[J]. Plant Diversity, 2007, 29(02): 247-250.

LIAO Jun-Jie^, , WU Ying-Jie ^{* *} , YAN Lung-Fei. Biophysical and Pharmacological Characterization of a Dynamin-like Protein from Day-lily ( Hemerocallis fulva , Liliaceae) Pollens[J]. Plant Diversity, 2007, 29(02): 247-250.

[1]	廖俊杰; 吴英杰; 阎隆飞 . 萱草花粉中微管蛋白生物化学性质[J]. Plant Diversity, 2006, 28(04): 425-428.
[2]	廖俊杰1,2 , 吴英杰1 , 阎隆飞1 . 萱草花粉微管蛋白的体外聚合及电镜观察[J]. Plant Diversity, 2005, 27(06): 649-652.