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Plant Diversity ›› 1998, Vol. 20 ›› Issue (01): 1-3.

• 研究论文 •    

知母雌雄配子体发生及胼胝质动态的研究

李师翁   

  1. 庆阳师范高等专科学校生物系,甘肃西峰745000
  • 出版日期:1998-02-25 发布日期:1998-02-25

Studies on the Development of Male- and Female- Gametophytes and Callose Deposit Dynamics in Anmearrhean asphodeloides

LI Shi-Weng   

  1. Deportment of Biology QingYang Teacher's College, Xifeng Gansu 745000
  • Online:1998-02-25 Published:1998-02-25

摘要: 知母(Anemarrhena asphodeloides)小孢子母细胞在减数分裂早前期开始合成胼胝质,其在母细胞壁上的沉积无方向性且不均匀。小孢子四分体时胼胝质壁达最厚,小孢子四分体晚期衰减,单核小孢子在四分体时期即开始合成胼胝氏质壁,至收缩期胼胝氏质衰减,小孢子核有丝分裂前消失,生殖细胞具有胼胝氏质性质的细胞壁。大孢子母细胞在减数分裂早前期开始合成胼胝氏质壁,其在母细胞壁上的沉积具有较明显的方向性,三分体大孢子时期,胼胝质集中在三分体大孢子的横壁上,侧壁上则无。胼胝质由合点端功能大孢子向珠孔端非功能大孢子开始衰减,胚囊母细胞晚期消失,2一核胚囊期胼胝质荧光再次出现在胚囊壁上,并逐渐增强,至成熟胚囊期消失。研究发现,在雌雄配子体发生过程中,胼胝质的动态基本相一致。知母的胚囊发育为单孢子的蓼型,与文献报道相同。

关键词: 知母, 雌雄配子体发生, 胼胝质

Abstract: The callose appears in the early meiotic prophase in microspore mother cell walls, and deposite no directive. At early tetrad stage callose wall reach the thickest, and begin to disappear at late tetrad stage. Microspore begin to synthesis and depositon callose wall at tetrad phase. At spore-release phase the callose fluorescence is strong. It decline at mid-vacuolate phase, and dis-appear at pre-mitosis. Callose wall is occurs in the generative cell wall. The formation of callose in the walls during megasporogenesis is similar that in microsporogenesis. It appears in the early meiotic prophase. but there is a markly polarity, always first appear in wall of the chalazal part. At triads stage. callose occurs in the transverse wall, and no in sidewalls. Callose fluorescence de-creasc staiting from the chalazal end of functional megaspore, and disappear at late uninuclear embryo sac phase.

Key words: Anemarrhena asphodelodies, Male & female-gametophyte, Callose