天麻球茎中一种抗真菌蛋白的分离和部分特性

Plant Diversity ›› 1988, Vol. 10 ›› Issue (04): 1-3.

• 研究论文 •

天麻球茎中一种抗真菌蛋白的分离和部分特性

胡忠杨增明王钧

中国科学院昆明植物研究所

出版日期:1988-08-25 发布日期:1988-08-25

ISOLATION AND PARTIAL CHARACTERIZATION OF AN ANTIFUNGAL PROTEIN FROM GASTRODIA ELATA CORM

Hu Zhong, Yang Zengming,Wang Jun

Kunming Institute of Botany, Academia Sinica, Kunming;Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai

Online:1988-08-25 Published:1988-08-25

摘要/Abstract

摘要： 自天麻（Gastrodia elata）顶生球茎中分离并纯化了一种抗真菌蛋白（Gastrodia Antifungal Protein），简称GAFP，在马铃薯葡糖琼脂培养基上,4μg GAFP滴加在直径0.6 cm圆纸片上可明显抑制木霉（Trichoderma reesei）菌丝生长。从每千克鲜球茎中可分离得GAFP约20 mg. 用SDS－PAGE和凝胶过滤层析测得该蛋白为单多肽链，分子量14.okD；用离子交换结合法测得电点为8.1;富含Asn，Ala，Gly和Leu，但无 Met，Cys和Pro。未经热变性或SDS处理的GAFP与考马氏亮蓝试剂不发生显色反应。GAFP不具有几丁质酶和分β-1，3一葡聚糖酶活性。球茎外层组织富含这种蛋白，内层薄壁组织无此蛋白。认为GAFP在阻止真菌侵染当年生顶生和侧生球茎的防卫机制中起重要作用.

关键词: 天麻, 抗真菌蛋白

Abstract: An anti-fungal protein named GAFP was isolated and purified from the terminal corm of Gastrodia elata Bl. 4 p-g of this protein impregnated in a paper disc on potato glucose agar medium inhibited obviously mycelial spread of Trichoderma reessei, About 20 mg of GAFP was obtained from 1 kg of fresh corm. Only the external part of corm contains this protein, whereas no GAFP was detected in inner part of corm. SDS-PAGE and gel filtration on sephadex G50 showed that GAFP is a single polypetide with MW=14.0 kD, and pI =8.1, determined by binding test of the protein on SP-Sephadex C50. The protein is rich in Asn, Gly, Ala and Leu, but lacks Met, Pro and Cys. GAFP can not give colour reaction with Coomassie blue reagent except that the protein is dena-tured by heat or SDS treatment. It was shown not to be of exochitinase and β-1, 3-glucanase activity. GAFP is thought to play an important role in the defense mechanism of the yearly terminal corm to prevent the infection of fungi.

Key words: Gastrodia elata

胡忠杨增明王钧. 天麻球茎中一种抗真菌蛋白的分离和部分特性[J]. Plant Diversity, 1988, 10(04): 1-3.

Hu Zhong, Yang Zengming,Wang Jun. ISOLATION AND PARTIAL CHARACTERIZATION OF AN ANTIFUNGAL PROTEIN FROM GASTRODIA ELATA CORM[J]. Plant Diversity, 1988, 10(04): 1-3.

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天麻球茎中一种抗真菌蛋白的分离和部分特性

ISOLATION AND PARTIAL CHARACTERIZATION OF AN ANTIFUNGAL PROTEIN FROM GASTRODIA ELATA CORM

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