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Plant Diversity ›› 1981, Vol. 3 ›› Issue (02): 1-3.

• 研究论文 •    

佛手瓜简化培养基诱导小麦花粉植株的研究

黎继岚   

  1. 云南大学生物系
  • 出版日期:1981-04-25 发布日期:1981-04-25

A STUDY ON INDUCING WHEAT P0LLEN PLANT USING SIMPLIFIED CULTURE MEDIUM OF SECHIUM EDULE (JACQ. ) SW.

Li Chi-lan   

  1. Department of Biology,Yunnan University
  • Online:1981-04-25 Published:1981-04-25

摘要: 我们研究了佛手瓜简化培养基诱导小麦花粉植株;以马铃薯简化培养基(简称 “薯”)作为诱导愈伤组织的对照培养基和MS+吲哚乙酸0.5毫克/升+激动素2毫克/升(简称MS)作为愈伤组织分化的对照培养基。 愈伤组织的诱导结果是“薯”和“瓜+激素”两种培养基的诱导频率因不同材料而异。多数的材料是“薯”的诱导频率较高,但这两种培养基诱导的愈伤组织转移至MS分化培养基时,前者分化白苗率较高,后者分化绿苗率较高。值得注意的是“瓜”培养基诱导的愈伤组组频率高于“瓜+激素”。 愈伤组织的分化是“瓜(分)”对愈伤组织的分化频率仅为MS的二分之一;同时前者比后者分化绿苗率也较低。然而,“瓜(分)十Fe”与MS对愈伤组织的分化频率几乎相等,绿苗分化率前者高于后者。

关键词: 佛手瓜简化, 培养, 基诱导, 小麦, 花粉

Abstract: The induction of wheat (Triticum aestivum L.) pollen plant using simplified culture medium of Sechium edue (Jacg.) Sw. (table 1.) was studied For inducing callus, the simplified culture medium of potatot (abbr."potato") was used as the control midium. For inducing differentiating plantlets, we used the culture medium, MS + IAA 0.5mg/l+kinetin 2mg/1 (abbr. "MS" ) as the cohtrol medium. In inducing callus,the inducing frequencies of the two media, "potato"and "chayote + kinetin",were found to be different with different material (table 2, table 3.) .In many material, inducing frequency of "potato" is higher.However, when induced callus from the two culture media were transferred to differentiation culture medium "MS", more albino plants were differentiated; in the former and more green plants in the latter. It is noteworthy that the frequency of induced callus in "chayote" culture medium is higher than in "chayote + kinetin" (table 3).

Key words: inducing, pollen, plant, simplified