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Plant Diversity ›› 1986, Vol. 8 ›› Issue (02): 1-3.

• 研究论文 •    

实生成年苹果树外植体的快速微型繁殖

黄仕周 刘艾琴   

  1. 中国科学院昆明植物研究所
  • 出版日期:1986-04-25 发布日期:1986-04-25

RAPID MICR0-PR0PAGATI0N 0F EXPLANTS FR0M ADULT SEEDLING APPLE TREES IN VITR0

Huanq Shizhou and Liu Aiqin   

  1. Kunming Institute of Botany, Academia Sinica
  • Online:1986-04-25 Published:1986-04-25

PDF (PC)

2437

摘要： 试验结果表明：以实生成苹果树上的芽作为外植体，培养在添加1-2mg/1 6-BA的SH或MS培养基上，在继代培养时芽的萌发率达92%以上，50天内芽数增殖5-6.8倍；同期内，液体旋转培养比琼脂静置培养芽条长度要增加倍左右；芽条在0.2-0.4mg/1IBA中生根率80%以上；试管苗的移栽成活率85%以上。

关键词: 快速微型繁殖, 实生成年苹果树

Abstract: The experimental reslllt showed: In the mcdium of Murashigr and Shoog (MS) or Schenk and Hidebrandt (SH) added with 6-benzylamino-purine (6 -BA) at 1- 2 mg/l, the sprouting rate of buds in subculture of the buds from the adult seedling app1e trees were over 92%. Buds multiplied 5-6.8 time in fifty days. The length of shoots in a rotating liquid culture was about three times more than that in the culture on agar-medium. In the medium added with indole- 3 -butyric acid (IBA) at 0.2-0.4 mg/l, the ro-oting rate of shoots were over 80%. The suryiyal rate of transplanting tuble- plantlets were more than 85%.

Key words: Rapid micro-propagation

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