荒漠草原土壤氨氧化细菌群落结构对氮添加和枯落物输入的响应
- 1.宁夏大学西北退化生态系统恢复与重建教育部重点实验室, 宁夏大学西北土地退化与生态恢复国家重点实验室培育基地, 银川 750021
2.宁夏大学生态环境学院, 银川 750021
收稿日期: 2022-05-26
录用日期: 2022-09-28
网络出版日期: 2022-09-28
基金资助
国家自然科学基金(31960359);宁夏重点研发计划(2021BEG02005);第三批宁夏青年科技人才托举工程(TJGC2018068)
Response of ammonia oxidizing bacteria to nitrogen fertilization and plant litter input on desert steppe
- 1. Key Laboratory of Restoration and Reconstruction of Degraded Ecosystems in Northwestern China of Ministry of Education, Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwestern China, Ningxia University, Yinchuan 750021, China
2. School of Ecology and Environment, Ningxia University, Yinchuan 750021, China
Received date: 2022-05-26
Accepted date: 2022-09-28
Online published: 2022-09-28
Supported by
National Natural Science Foundation of China(31960359);Ningxia Huizu Autonomous Region Key Research and Development Project(2021BEG02005);Third Batch of Ningxia Young Scientific and Technological Talent Support Project(TJGC2018068)
摘要
为了解荒漠草原表层土壤氨氧化细菌(AOB)对枯落物输入及氮添加的响应, 该研究以宁夏回族自治区盐池县荒漠草原为实验平台, 选择4种常见植物——苦豆子(Sophora alopecuroides)、猪毛蒿(Artemisia scoparia)、短花针茅(Stipa breviflora)和沙芦草(Agropyron mongolicum)的枯落物设置枯落物输入处理, 设置对照(0 g·m-2·a-1)、氮添加(9.2 g·m-2·a-1) 2个处理, 运用荧光定量PCR、高通量测序等分子生物学技术, 探究了荒漠草原0-5 cm表层土壤AOB对氮添加和不同枯落物输入的响应。研究结果显示, 在氮添加和枯落物输入条件下, 荒漠草原土壤表层AOB共有3门4纲6目7科8属17种。实验所测AOB群落物种主要来源于β-变形菌纲(beta-Proteobacteria)中的亚硝基单胞菌属(Nitrosomonas)和亚硝基螺旋菌属(Nitrosospira), 且以亚硝基单胞菌属为优势种群。与对照相比, 氮添加显著降低了AOB-氨单加氧酶(amoA)基因拷贝数, 表明施氮会抑制荒漠草原表层土壤中的硝化作用。在不同种类的枯落物输入下, AOB-amoA基因丰度的响应具有差异性, 枯落物输入能够在一定程度上缓解氮添加对土壤AOB-amoA基因丰度的抑制, 但总体上不会改变其变化趋势。冗余分析结果表明土壤有机碳、全磷、硝态氮和铵态氮含量是影响AOB-amoA基因丰度的主要土壤理化因子。研究结果表明, 氮添加能够显著降低荒漠草原土壤表层AOB-amoA的基因丰度, 该结果有助于丰富和拓展未来干旱半干旱荒漠草原地区氮沉降变化对土壤表层氮循环功能基因丰度的影响和机制的认识。
本文引用格式
张雅琪, 庞丹波, 陈林, 曹萌豪, 何文强, 李学斌 . 荒漠草原土壤氨氧化细菌群落结构对氮添加和枯落物输入的响应[J]. 植物生态学报, 2023 , 47(5) : 699 -712 . DOI: 10.17521/cjpe.2022.0220
Abstract
Aims This study aimed to explore the response of ammonia oxidizing bacteria (AOB) to litter decomposition and nitrogen application in the topsoil of desert steppe, and the main environmental factors influencing the AOB were also analyzed. Thus, this study will contribute to better understanding of the mechanism of nitrogen cycle function gene abundance in response to nitrogen deposition in arid and semi-arid desert steppe regions.
Methods We conducted the nitrogen fertilization experiment in the desert steppe in Yanchi, Ningxia. The four plant litters, including Sophora alopecuroides, Artemisia scoparia, Stipa breviflora and Agropyron mongolicum were selected as plant litter input treatments. The nitrogen fertilization treatments, including control (0 g·m-2·a-1) and nitrogen application (9.2 g·m-2·a-1), were applied to explore the response of AOB to nitrogen deposition and different litter inputs in top soil (0-5 cm) of desert steppe by fluorescent quantitative PCR and high-throughput sequencing.
Important findings Our results showed that there were 3 phyla, 4 classes, 6 orders, 7 families, 8 genera and 17 species of AOB in the topsoil of desert steppe under nitrogen application and litter decomposition. The AOB communities were mainly derived from Nitrosomonas and Nitrosospira, which were from beta-Proteobacteria, and Nitrosospira was the dominant species. Compared with the control, the gene copy number of AOB was significantly decreased under nitrogen application, indicating that nitrogen application inhibited nitrification in the topsoil of the desert steppe. Whereas, the response of AOB-ammonia monooxy genase subsuit A (amoA) gene abundance varied under different plant litter decomposition. Litter input could alleviate the inhibition of nitrogen enrichment on soil AOB-amoA gene abundance to a certain extent, but did not change the trend of soil AOB-amoA gene abundance. A redundancy analysis confirmed that soil organic carbon, total phosphorus, ammonium nitrogen and nitrate nitrogen contents were the key environmental factors affecting niche separation of AOB-amoA gene abundance in desert steppe soil. The results showed that nitrogen addition could significantly reduce the gene abundance of AOB-amoA, thereby affecting the nitrification and the direction of soil nitrogen transformation in the topsoil of desert steppe.
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