Abstract: Transformed crown tissue cultures from Trichosanthes kirilowii Maxim. were established by Ti plasmid in C58 strains. The crown issues grew well in free- hormone MS medium . Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully .The content of proteins in the tissue was about 130.6mg/g (fresh weight)which was inspected by compound-color methods and its TCN-protein was inspected by SDS-PAGE analysis. The study showed that the production of TCN proteins from crown tissue cultures may be developed in prospect.

Key words: Trichosanthes kirilowii

摘要： 用根癌农杆菌侵染栝楼（Trichosanthes kirilowii Maxim.）无菌苗后，获得其冠瘿组织；栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，并合成冠瘿碱，表明Ti质粒转化成功。栝楼冠瘿组织中最高蛋白含量为130.6mg/g(鲜重)。经SDS－PAGE检测其含有的蛋白种类与栝楼根含有的基本一样。研究表明，利用栝楼冠瘿组织作为培养系统生产天花粉蛋白有很好的开发前景。

关键词: 栝楼, Ti质粒转化, 冠瘿组织培养