丛枝菌根真菌根外菌丝跨膜H <sup>+</sup>和Ca <sup>2+</sup>流对干旱胁迫的响应

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丛枝菌根真菌根外菌丝跨膜H ⁺和Ca ²⁺流对干旱胁迫的响应

徐丽娇^1,²,郝志鹏¹,谢伟^1,²,李芳^1,²,陈保冬^1,^2,^*(

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Transmembrane H ⁺ and Ca ²⁺ fluxes through extraradical hyphae of arbuscular mycorrhizal fungi in response to drought stress

XU Li-Jiao^1,2, HAO Zhi-Peng¹, XIE Wei^1,2, LI Fang^1,2, CHEN Bao-Dong^1,2,*(

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图1. 丛枝菌根真菌-胡萝卜毛状根分室培养系统示意图。左边是菌根室(mycorrhizal compartment), 右边是菌丝室(hyphal compartment)。菌根室中加入用质量浓度0.4%的植物凝胶固化后的M培养基, 并接入菌根化的转移Ri T-DNA胡萝卜毛状根; 菌丝室加入液体M培养基, 以供根外菌丝生长(参考St-Arnaud et al., 1996)。

Fig. 1. Diagram of the two-compartments in vitro culture system of arbuscular mycorrhizal fungi with hairy carrot root. Mycorrhizal compartment was filled with solid M medium gelled with 0.4% phytagel, allowing development of mycorrhizal roots; extraradical mycelium ramified into hyphal compartment filled with liquid M medium without sucrose and phytagel, and the roots that crossed the central wall were trimmed to prevent their growth in hyphal compartment (referred to St-Arnaud et al., 1996).