中性糖在土壤中的来源与分布特征

刘程竹,贾娟,戴国华,马田,冯晓娟

Origin and distribution of neutral sugars in soils

LIU Cheng-Zhu,JIA Juan,DAI Guo-Hua,MA Tian,FENG Xiao-Juan

表1 土壤中性糖的提取及检测方法比较

Table 1 Comparison of extraction and detection methods of neutral sugars in soils

步骤 Procedure 方法 Method 优点 Advantage 缺点 Drawback 参考文献 Reference 提取 Extraction 硫酸 H2SO4 硫酸不易被除去 H2SO4 can not be removed easily Tanaka et al., 1990 盐酸 HCl 会水解一部分纤维素; 产率较低 The hydrolysis products include a few cellulosic neutral sugars; low yields Uzaki & Ishiwatari, 1983 三氟乙酸 TFA 产率高; 不会破坏单糖结构; 具有挥发性, 可通过旋转蒸发去除; 水解的多糖主要为半纤维素 High yields; Not destructive to monosaccharides; TFA is volatile and can be easily removed by evaporation; Hydrolysis products are mainly released from hemicellulose Amelung et al., 1996 检测 Detection GC-MS 精度、准确度、敏感性和效率较高 High accuracy, precision, sensibility and efficiency 需要衍生化 Derivatization is required Amelung et al., 1996; Wang et al., 2017 HPLC 无需衍生化; 纯化过程简单 No need for derivatization; Simple purification procedures 精度、准确度、敏感性较低 Low accuracy, precision, sensibility and efficiency Hamada & Ono, 1984; Angers et al., 1988;Tanaka et al., 1990; HPAEC-PAD 无需衍生化; 应用范围广, 可同时分析糖醛酸和中性糖 No need for derivatization; Wide application and simultaneous analysis of uronic acid and neutral sugars 精度、准确度、敏感性较低 Low accuracy, precision, sensibility and efficiency Bruggink et al., 2005; Zhang et al., 2012