植物硒生物强化及微生物在其中的应用潜力

李柳, 刘庆华, 尹春英

Selenium biofortification in plants and application potential of microorganisms in selenium biofortification

LI Liu, LIU Qing-Hua, YIN Chun-Ying

表1 微生物对植物硒(Se)吸收影响的相关研究

Table 1 Research on the effect of microorganisms on the absorption of selenium (Se) in plants

微生物种类 Microbial type 寄主植物 Host plant 微生物名称 Microbes 施肥方式 Fertilization method 硒形态 Selenium form 结论 Result 参考文献 Reference AMF 玉米 Zea mays 摩西球囊霉 Glomus mosseae 土壤施肥 Soil fertilization Se(IV) Se(VI) 用Se(IV)处理时, 接种AMF减少了有机硒在玉米中的积累, 用Se(VI)处理时, 接种AMF对玉米中的硒含量无明显影响 When treated with Se(IV), inoculation with AMF reduced organic selenium accumulation in maize, and when treated with Se(VI), inoculation with AMF had no significant effect on selenium content in maize Yu et al., 2011 莴苣 Lactuca sativa 根内根孢囊霉 和摩西管柄囊霉 Rhizophagus intraradices and Funneliformis mosseae 土壤施肥 Soil fertilization Se(IV) SeCH3 SeU 接种AMF减少了硒在莴苣叶片中的积累 Inoculation with AMF reduced selenium accumulation in lettuce leaves Goicoechea et al., 2015 小麦 Triticum aestivum 变形球囊霉或摩西管柄囊霉 Glomus versiforme or Funneliformis mosseae 土壤施肥 Soil fertilization Se(IV) Se(VI) SeMet 接种AMF提高了冬小麦对Se(IV)、Se(VI)吸收, 对SeMet的吸收无影响 Inoculation with AMF increased the absorption of Se(IV) and Se(VI) in winter wheat, but had no effect on the absorption of SeMet Luo et al., 2019 石刁柏 Asparagus officinalis 根内根孢囊霉和摩西管柄囊霉 Rhizophagus intraradices and Funneliformis mosseae 叶面施肥 Foliage fertilization Se(VI) 接种AMF使石刁柏中硒水平提高11.8倍, 提升了叶面施用硒肥的效率 Inoculation with AMF increased the level of selenium in spears of Asparagus officinalis 11.8 times, and significantly improved the efficiency of foliar selenium application Conversa et al., 2019 紫花苜蓿 Medicago sativa 摩西球囊霉 Glomus mosseae 土壤施肥 Soil fertilization Se (IV) 在低磷条件下, 接种AMF促进了苜蓿根系对硒的吸收 Under low phosphorus conditions, inoculation with AMF promoted the uptake of selenium by alfalfa roots Peng et al., 2020 水稻 Oryza sativa 变形球囊霉和摩西管柄囊霉 Glomus versiforme and Funneliformis mosseae 土壤施肥 Soil fertilization Se(IV) Se(VI) 接种AMF可能会改变微生物群落, 将土壤中的无效硒转变为有效硒而提高硒的吸收, 接种AMF能促进水稻籽粒中有机硒的积累 Inoculation with AMF may changethe microbial community, convert unavailable selenium to available selenium in soil and improve selenium absorption. Inoculation with AMF promote organic selenium accumulation in rice grain Chen et al., 2020 大蒜 Allium sativum 变形球囊霉 Glomus versiforme 叶面施肥 Foliage fertilization Se(IV) 在高浓度(50 mg·L-1)硒含量下, 接种AMF明显降低了植物对硒的吸收和转运 Under high selenium content (50 mg·L-1), inoculation with AMF significantly reduced the absorption and transport of selenium by plants Yang et al., 2021 ECM 马尾松 Pinus massoniana 褐环乳牛肝菌 Suillus luteus - - 在低重金属污染的土壤中, 马尾松接种ECM后, 其植物中的平均硒含量显著增大 In low level heavy metal pollution soil, the mean selenium content in Pinus massoniana increased significantly after inoculation with ECM Sun et al., 2020 PGPR 小麦 Triticum aestivum 假单胞菌属R8和寡养单胞菌属B19 Pseudomonas sp. R8 and Stenotrophomonas sp. B19 土壤施肥 Soil fertilization Se(IV) 接种耐硒细菌促进了硒从小麦的根部到叶片的运输 Inoculation with selenium-tolerant bacteria promoted selenium transport from roots to leaves in wheat Acu?a et al., 2012 小麦 Triticum aestivum 蜡样芽孢杆菌-YAP6和 地衣芽孢杆菌-YAP7 Bacillus Cereus-YAP6 and Bacillus Licheniformis-YAP7 土壤施肥 Soil fertilization Se(VI) 接种菌株YAP6和YAP7是一种很好的小麦硒生物强化策略 Inoculation with strains YAP6 and YAP7 is a good strategy for selenium biofortification in wheat Yasin et al., 2015a 小麦 Triticum aestivum Bacillus pichinotyi YAM2 土壤施肥 Soil fertilization Se(VI) Bacillus pichinotyi YAM2在硒胁迫和缺硒条件下均能促进小麦生长, 并提高小麦中硒的含量 Bacillus pichinotyi YAM2 promoted the growth of wheat and increased the content of selenium in wheat under both selenium stress and selenium deficiency conditions Yasin et al., 2015b 甘蓝 Brassica oleracea 假单胞菌属T5-6-I Pseudomonas sp. strain T5-6-I 培养基施肥 Medium fertilization Se(IV) Pseudomonas sp. strain T5-6-I的接种能够提高硒在甘蓝中的积累, 但是并没有影响硒在甘蓝中的存在形态 The inoculation of Pseudomonas sp. strain T5-6-I could increase the accumulation of selenium in cabbage, but did not affect the existence of selenium in cabbage Lusa et al., 2019 白菜 Brassica campestris 农杆菌属T3F4 Agrobacterium sp. T3F4 土壤施肥 Soil fertilization Se0 Se(IV) Agrobacterium sp. T3F4能够将土壤中的0价硒转变为+4价的硒, 从而促进白菜对硒的吸收 Agrobacterium sp. T3F4 could convert 0-valent selenium in soil to +4-valent selenium, thereby promoting the absorption of selenium by cabbage Zhu et al., 2021 水稻 Oryza sativa 噬几丁质菌属和睾丸酮丛毛单胞菌 Chitinophaga sp. and Comamonas testosteroni - - Chitinophaga sp.和Comamonas testosteroni均能有效还原Se (IV), 生成硒纳米粒子, 都能提高土壤中的有效硒和水稻植株中的硒含量 Both Chitinophaga sp. and Comamonas testosteroni effectively reduced Se(IV) and generated selenium nanoparticles, both of which can increase the available selenium in soil and the selenium content in rice plants Huang et al., 2021