七彩花生根瘤内生菌对氮添加的响应及其与植物化学计量特征的关系

李林, 孙毅, 杨晓琼, 方海东, 闫帮国

Response of endophytes in root nodules of Arachis hypogaea ‘Qicai’ to nitrogen addition and its relationship with plant stoichiometry characteristics

LI Lin, SUN Yi, YANG Xiao-Qiong, FANG Hai-Dong, YAN Bang-Guo

图3 PICRUSt 2预测根瘤内生菌酶功能对施氮量的响应及其与叶片化学计量特征的关系。A, 酶活性变化(平均值±标准差)。B, 酶活性主坐标分析(PCoA)。C, 酶活性与叶片化学计量特征的关系。N0, 0 kg·hm-2; N1, 140 kg·hm-2; N2, 280 kg·hm-2。C, 碳; N, 氮; P, 磷。3-Phytase, 3-植酸酶; 4-Phytase, 4-植酸酶; Acid phosphatase, 酸性磷酸酶; Alkaline phosphatase, 碱性磷酸酶; Alpha-amylase, α-淀粉酶; Alpha-glucosidase, α-糖苷酶; Ammonia monooxygenase, 氨单加氧酶; Beta-glucosidase, β-糖苷酶; Cellulase, 纤维素酶; Chitinase, 几丁质酶; Nitrate reductase, 硝酸还原酶; Nitric oxide dioxygenase, 一氧化氮双加氧酶; Nitrite reductase (NADH), 亚硝酸还原酶(NADH); Nitrite reductase (NO-forming), 亚硝酸盐还原酶(NO-生成); Nitronate monooxygenase, 硝基单加氧酶; Nitrous-oxide reductase, 一氧化二氮还原酶; Nitrogenase, 固氮酶。*, p ≤ 0.05; **, p ≤ 0.01。A中不同小写字母表示不同处理间差异显著(p < 0.05)。

Fig. 3 Response of root nodule endophyte enzyme functions (predicted by PICRUSt 2) to nitrogen addition treatments and their association with leaf stoichiometric characteristics. A, Changes in enzyme activities (mean ± SE). B, Principal component analysis (PCoA) of enzyme activity. C, Relationships between enzyme activities and leaf stoichiometric characteristics. N0, 0 kg·hm-2; N1, 140 kg·hm-2; N2, 280 kg·hm-2. C, carbon; N, nitrogen; P, phosphorus。*, p ≤ 0.05; **, p ≤ 0.01. Different lowercase letters in A indicate significant differences among treatments (p < 0.05).