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植物生态学报 >

2011 , Vol. 35 >Issue 3: 268 - 274

DOI: https://doi.org/10.3724/SP.J.1258.2011.00268

研究论文

低温胁迫后苔藓植物对模拟氮沉降条件的生理响应

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  • 1中山大学生命科学学院, 广州 510275
    2暨南大学生命科学与技术学院, 广州 510632
    3广东省黑石顶自然保护区, 广东肇庆 526536
*E-mail: lsslwq@mail.sysu.edu.cn

收稿日期: 2010-05-06

  录用日期: 2010-09-15

  网络出版日期: 2011-03-02

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Physiological responses of bryophytes experienced low temperature stress to simulated nitrogen deposition

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  • 1School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
    2School of Life Science and Technology, Jinan University, Guangzhou 510632, China
    3Heishiding Nature Reserve, Zhaoqing, Guangdong 526536, China

Received date: 2010-05-06

  Accepted date: 2010-09-15

  Online published: 2011-03-02

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摘要

2008年初受强寒潮的影响, 中国华南大部分地区出现持续的异常低温。该文研究了华南地区常见的3种苔藓植物 ——刺边小金发藓拟刺亚种(Pogonatum cirratum subsp. fuscatum)、大灰藓(Hypnum plumaeforme)和石地钱(Reboulia hemisphaerica), 在人工模拟氮(N)沉降两年并经历2008年初异常低温气候后的生理响应变化, 并与2007年正常气候情况下人工加N一年后的结果进行比较, 分析苔藓植物的生长与N沉降之间的关系, 并探讨N沉降对低温胁迫后苔藓植物的补偿生长的影响。结果显示: 2008年3种苔藓植物的净光合速率和淀粉含量在加N量为0-60 kg N·hm -2·a-1的范围内均随着N浓度的上升而下降, 总N含量在加N量处于0-40 kg N·hm-2·a-1的范围内随着N浓度的上升而上升, 至60 kg N·hm-2·a-1时不再上升, 甚至有所下降。2008年, 3种苔藓植物大多数碳氮代谢指标在对照及低N条件下与2007年加N 1年且在正常气候时同种N处理时相比均有不同程度的上升, 但上升幅度与加N浓度成反比, 至中高N条件时两者常较接近, 显示苔藓植物在经历低温胁迫后会出现超补偿效应, 但是在N沉降升高的条件下, 补偿生长能力下降。

关键词： 苔藓植物; 补偿效应; 低温胁迫; 氮沉降

本文引用格式

刘滨扬, 刘蔚秋, 张以顺, 雷纯义 . 低温胁迫后苔藓植物对模拟氮沉降条件的生理响应[J]. 植物生态学报, 2011 , 35(3) : 268 -274 . DOI: 10.3724/SP.J.1258.2011.00268

Abstract

Aims Increasing N deposition and frequent abnormal weather are two characteristics of global climate change. Our objective was to study the physiological responses of bryophytes to simulated nitrogen treatments and low temperature stress to provide insights to the relationship between N deposition and compensatory effect after low temperature stress.
Methods Physiological responses of three bryophyte species, Pogonatum cirratum subsp. fuscatum, Hypnum plumaeforme and Reboulia hemisphaerica, which were subjected to a 2-year-simulated N deposition and suffered a low temperature stress in early 2008, were studied and compared with the results of the bryophytes that experienced a 1-year-simulated N deposition and normal weather conditions. N treatments (Control, 20, 40 and 60 kg N·hm-2·a-1) in three replicates were established for each species. The N additions were divided into four applications per year.
Important findings The results of 2008 showed that net photosynthetic rate and concentration of starch decreased with increasing N addition doses within the range of 0-60 kg N·hm-2·a-1. The concentration of total N increased with increasing N treatment doses within the range of 0-40 kg N·hm-2·a-1, but decreased at N addition doses of 60 kg N·hm-2·a-1. At control and low N addition conditions (20 kg N·hm-2·a-1), most of the indices of carbon and nitrogen metabolism of the three bryophytes were higher than the results of 2007 of the same species at the same N treatments, but N addition depressed the increase. At high N treatment conditions (≥ 40 kg N·hm-2·a-1), the results of 2007 and 2008 were usually similar. Results indicate that the bryophytes exhibit overcompensatory growth after experiencing a low temperature stress, but at stimulated N deposition conditions, their compensatory ability decreases.

Key words： bryophytes; compensatory effect; low temperature stress; nitrogen deposition

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参考文献

[1] Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
[2] Cox SE, Stushnoff C (2001). Temperature-related shifts in soluble carbohydrate content during dormancy and cold acclimation in Populus tremuloides. Canadian Journal of Forest Research, 31, 730-737.
[3] Dionne J, Castonguay Y, Nadeau P, Desjardins Y (2001). Freezing tolerance and carbohydrate changes during cold acclimation of green-type annual bluegrass ( Poa annua L.) ecotypes. Crop Science, 41, 443-451.
[4] Fang YT, Yoh M, Koba K, Zhu WX, Takebayashi Y, Xiao YH, Lei CY, Mo JM, Zhang W, Lu XK (2011). Nitrogen deposition and forest nitrogen cycling along an urban-rural transect in southern China. Global Change Biology, doi: 10.1111/j.1365-2486.2010.02283.x(in Press)
[5] Frank DA, Evans RD (1997). Effects of native grazers on grassland N cycling in Yellowstone National Park. Ecology, 78 , 2238-2248.
[6] Freer-Swith PH, Mansfield TA (1987). The combined effects of low temperature and SO2+NO2 pollution on the new season’s growth and water relation of Picea sitchensis. New Phytologist, 106, 237-250.
[7] Galloway JN, Cowling EB (2002). Reactive nitrogen and the world: 200 years of change. Ambio, 23, 120-123.
[8] Hou FJ (侯扶江), Li G (李广), Chang SH (常生华) (2002). Physiological indices of grazed grassland under health management. Chinese Journal of Applied Ecology (应用生态学报), 13, 1049-1053. (in Chinese with English abstract)
[9] Huhta AP, Hellstr?m K, Rautio P, Tuomi J (2000). A test of the compensatory continuum: fertilization increases and below-ground competition decreases the grazing tolerance of tall wormseed mustard ( Erysimum strictum). Evolutionary Ecology, 14, 353-372.
[10] Lee JA (1998). Unintentional experiments with terrestrial ecosystems: ecological effects of sulphur and nitrogen pollutants. Journal of Ecology, 86, 1-12.
[11] Li ZH, Piippo S (1994). Preliminary list of bryophytes of Heishiding Nature Reserve, Guangdong Province, China. Tropical Bryology, 9, 35-41.
[12] Liu BY (刘滨扬), Liu WQ (刘蔚秋), Lei CY (雷纯义), Zhang YS (张以顺) (2009). Physiological responses of three bryophyte species of south China to simulated nitrogen deposition. Chinese Journal of Plant Ecology (植物生态学报), 33, 141-149. (in Chinese with English abstract)
[13] Liu WQ (刘蔚秋), Liu BY (刘滨扬), Wang J (王江), Lei CY (雷纯义) (2010). Responses of soil microbial communities to moss cover and nitrogen addition. Acta Ecologica Sinica (生态学报), 30, 1691-1698. (in Chinese with English abstract)
[14] Liu XE (刘雄恩), Wang BS (王伯荪) (1987). The vegetation classification system and main kinds of their distribution in Heishiding Nature Reserve. Ecological Science (生态科学), 1(2), 19-34. (in Chinese with English abstract)
[15] Margolis HA, Waring RH (1986). Carbon and nitrogen allocation pattern of Douglas-fir seedlings fertilized with nitrogen in autumn. Canadian Journal of Forest Research, 16, 897-910.
[16] Maschinski J, Whitham TG (1989). The continuum of plant responses to herbivory: the influence of plant association, nutrient availability and timing. The American Naturalist, 134, 1-19.
[17] Nowak RS, Caldwell MM (1984). A test of compensatory photosynthesis in the field: implications for herbivory tolerance. Oecologia, 61, 311-318.
[18] Parker J (1962). Relationships among cold hardiness, water- soluble protein, anthocyanins, and free sugars in Hedera helix L. Plant Physiology, 37, 809-813.
[19] Pearce ISK, van der Wal R (2002). Effect of nitrogen deposition on growth and survival of montane Racomitrium lanuginosum health. Biological Conservation, 104, 83-89.
[20] Pearce ISK, Woodin SJ, van der Wal R (2003). Physiological and growth responses of the montane bryophyte Racomitrium lanuginosum to atmospheric nitrogen deposition. New Phytologist, 160, 145-155.
[21] Pearson J, Stewart GR (1993). The deposition of atmospheric ammonia and its effects on plants. New Phytologist, 125, 283-305.
[22] Schnyder H, de Visser R (1999). Fluxes of reserve-derived and currently assimilated carbon and nitrogen in perennial ryegrass recovering from defoliation—the regrowing tiller and its component functionally distinct zones. Plant Physiology, 119, 1423-1436.
[23] Soares A, Pearson J (1997). Short-term physiological responses of mosses to atmospheric ammonium and nitrate. Water, Air and Soil Pollution, 93, 225-254.
[24] Thornton B, Millard P (1996). Nitrogen uptake by grasses: changes induced by competing neighbour plants differing in frequency of defoliation. Grass and Forage Science, 51, 242-249.
[25] Tomassen HBM, Smolders AJP, Lamers LPM, Roelofs JGM (2003). Stimulated growth of Betula pubescens and Molinia caerulea on ombrotrophic bogs: role of high levels of atmospheric nitrogen deposition. Journal of Ecology, 91, 357-370.
[26] Touchette BW, Burkholder JM (2007). Carbon and nitrogen metabolism in the seagrass, Zostera marina L.: environmental control of enzymes involved in carbon allocation and nitrogen assimilation. Journal of Experimental Marine Biology and Ecology, 350, 216-253.
[27] Vitousek PM, Aber JD, Howarth RW, Likens GE, Matson PA, Schindler DW, Schlesinger WH, Tilman DG (1997). Human alteration of the global nitrogen cycle: sources and consequences. Ecological Applications, 7, 737-750.
[28] Zhao SJ (赵世杰), Liu HS (刘华山), Dong XC (董新纯) (1998). Experimental Guidance for Plant Physiology (植物生理学实验指导). Chinese Agricultural Science and Technology Press, Beijing. (in Chinese)
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