植物生态学报 ›› 2011, Vol. 35 ›› Issue (3): 268-274.DOI: 10.3724/SP.J.1258.2011.00268
刘滨扬1,2, 刘蔚秋1,*, 张以顺1, 雷纯义3
收稿日期:
2010-05-06
接受日期:
2010-09-15
出版日期:
2011-05-06
发布日期:
2011-03-02
通讯作者:
刘蔚秋
作者简介:
*E-mail: lsslwq@mail.sysu.edu.cnLIU Bin-Yang1,2, LIU Wei-Qiu1,*, ZHANG Yi-Shun1, LEI Chun-Yi3
Received:
2010-05-06
Accepted:
2010-09-15
Online:
2011-05-06
Published:
2011-03-02
Contact:
LIU Wei-Qiu
摘要:
2008年初受强寒潮的影响, 中国华南大部分地区出现持续的异常低温。该文研究了华南地区常见的3种苔藓植物 ——刺边小金发藓拟刺亚种(Pogonatum cirratum subsp. fuscatum)、大灰藓(Hypnum plumaeforme)和石地钱(Reboulia hemisphaerica), 在人工模拟氮(N)沉降两年并经历2008年初异常低温气候后的生理响应变化, 并与2007年正常气候情况下人工加N一年后的结果进行比较, 分析苔藓植物的生长与N沉降之间的关系, 并探讨N沉降对低温胁迫后苔藓植物的补偿生长的影响。结果显示: 2008年3种苔藓植物的净光合速率和淀粉含量在加N量为0-60 kg N·hm -2·a-1的范围内均随着N浓度的上升而下降, 总N含量在加N量处于0-40 kg N·hm-2·a-1的范围内随着N浓度的上升而上升, 至60 kg N·hm-2·a-1时不再上升, 甚至有所下降。2008年, 3种苔藓植物大多数碳氮代谢指标在对照及低N条件下与2007年加N 1年且在正常气候时同种N处理时相比均有不同程度的上升, 但上升幅度与加N浓度成反比, 至中高N条件时两者常较接近, 显示苔藓植物在经历低温胁迫后会出现超补偿效应, 但是在N沉降升高的条件下, 补偿生长能力下降。
刘滨扬, 刘蔚秋, 张以顺, 雷纯义. 低温胁迫后苔藓植物对模拟氮沉降条件的生理响应. 植物生态学报, 2011, 35(3): 268-274. DOI: 10.3724/SP.J.1258.2011.00268
LIU Bin-Yang, LIU Wei-Qiu, ZHANG Yi-Shun, LEI Chun-Yi. Physiological responses of bryophytes experienced low temperature stress to simulated nitrogen deposition. Chinese Journal of Plant Ecology, 2011, 35(3): 268-274. DOI: 10.3724/SP.J.1258.2011.00268
图1 2007及2008年生理指标测定前40天黑石顶自然保护区日平均气温(A)、日最高气温(B)和日最低气温(C)。数据来自黑石顶自然保护区气象监测站。图中实线代表2006年11月25日到2007年1月3日的数据; 虚线代表2008年1月31日到2008年3月8日的数据。
Fig. 1 Daily mean air temperature (A), daily maximum air temperature (B), daily minimum air temperature (C) at Heishiding Nature Reserve for 40 days before the measurement of physiological indices in 2007 and 2008. Data collected from the meteorological monitoring station at Heishiding Nature Reserve. The real lines represent data from Nov. 25, 2006 to Jan. 3, 2007, the dashed lines represent data from Jan. 31, 2008 to Mar. 8, 2008.
图2 模拟氮沉降两年后苔藓植物的碳代谢指标(平均值±标准偏差, n = 3)。A、D, 刺边小金发藓拟刺亚种。B、E, 大灰藓。C、F, 石地钱。在各物种和变量内, 具不同字母的数值间差异显著(p < 0.05, One-way ANOVA)。
Fig. 2 Indices of carbon metabolism in bryophytes after two years of simulated nitrogen deposition (mean ± SD, n = 3). A and D, Pogonatum cirratum subsp. fuscatum. B and E, Hypnum plumaeforme. C and F, Reboulia hemisphaerica. Values with different letters are significantly different (p < 0.05, One-way ANOVA) in each species and each dependent variable.
图3 2008年模拟N沉降两年后3种苔藓植物的N代谢指标(平均值±标准偏差, n = 3)。A, 刺边小金发藓拟刺亚种。B, 大灰藓。C, 石地钱。
Fig. 3 Indices of N metabolism in three bryophyte species in 2008 after two years of simulated nitrogen deposition (mean ± SD, n = 3). A, Pogonatum cirratum subsp. fuscatum. B, Hypnum plumaeforme. C, Reboulia hemisphaerica.
加N量 N addition doses (kg N·hm-2·a-1) | NRA (μg N·g-1 FW·h-1) | |
---|---|---|
固有活性 Constitutive NRA | 诱导活性 Inducible NRA | |
刺边小金发藓拟刺亚种 P. cirratum subsp. fuscatum | ||
0 | 3.86 ± 0.06a | 2.82 ± 0.48a |
20 | 1.80 ± 0.15b | -0.34 ± 0.51b |
40 | 1.04 ± 0.20c | -0.59 ± 0.10b |
60 | 0.54 ± 0.28d | -0.54 ± 0.33b |
大灰藓 H. plumaeforme | ||
0 | 3.86 ± 0.28a | 14.14 ± 0.70a |
20 | 1.58 ± 0.17b | 1.18 ± 0.13b |
40 | 1.48 ± 0.42b | -0.22 ± 0.02c |
60 | 1.14 ± 0.08b | -0.06 ± 0.18c |
表1 2008年模拟N沉降两年后刺边小金发藓拟刺亚种和大灰藓的硝酸还原酶活性(NRA) (平均值±标准偏差, n = 3)
Table 1 Nitrate reductase activity (NRA) in Pogonatum cirratum subsp. fuscatum and Hypnum plumaeforme in 2008 after two years of simulated nitrogen deposition (mean ± SD, n = 3)
加N量 N addition doses (kg N·hm-2·a-1) | NRA (μg N·g-1 FW·h-1) | |
---|---|---|
固有活性 Constitutive NRA | 诱导活性 Inducible NRA | |
刺边小金发藓拟刺亚种 P. cirratum subsp. fuscatum | ||
0 | 3.86 ± 0.06a | 2.82 ± 0.48a |
20 | 1.80 ± 0.15b | -0.34 ± 0.51b |
40 | 1.04 ± 0.20c | -0.59 ± 0.10b |
60 | 0.54 ± 0.28d | -0.54 ± 0.33b |
大灰藓 H. plumaeforme | ||
0 | 3.86 ± 0.28a | 14.14 ± 0.70a |
20 | 1.58 ± 0.17b | 1.18 ± 0.13b |
40 | 1.48 ± 0.42b | -0.22 ± 0.02c |
60 | 1.14 ± 0.08b | -0.06 ± 0.18c |
[1] |
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
DOI URL PMID |
[2] |
Cox SE, Stushnoff C (2001). Temperature-related shifts in soluble carbohydrate content during dormancy and cold acclimation in Populus tremuloides. Canadian Journal of Forest Research, 31, 730-737.
DOI URL |
[3] |
Dionne J, Castonguay Y, Nadeau P, Desjardins Y (2001). Freezing tolerance and carbohydrate changes during cold acclimation of green-type annual bluegrass ( Poa annua L.) ecotypes. Crop Science, 41, 443-451.
DOI URL |
[4] | Fang YT, Yoh M, Koba K, Zhu WX, Takebayashi Y, Xiao YH, Lei CY, Mo JM, Zhang W, Lu XK (2011). Nitrogen deposition and forest nitrogen cycling along an urban-rural transect in southern China. Global Change Biology, doi: 10.1111/j.1365-2486.2010.02283.x(in Press) |
[5] |
Frank DA, Evans RD (1997). Effects of native grazers on grassland N cycling in Yellowstone National Park. Ecology, 78 , 2238-2248.
DOI URL |
[6] |
Freer-Swith PH, Mansfield TA (1987). The combined effects of low temperature and SO2+NO2 pollution on the new season’s growth and water relation of Picea sitchensis. New Phytologist, 106, 237-250.
DOI URL |
[7] | Galloway JN, Cowling EB (2002). Reactive nitrogen and the world: 200 years of change. Ambio, 23, 120-123. |
[8] | Hou FJ (侯扶江), Li G (李广), Chang SH (常生华) (2002). Physiological indices of grazed grassland under health management. Chinese Journal of Applied Ecology (应用生态学报), 13, 1049-1053. (in Chinese with English abstract) |
[9] |
Huhta AP, Hellström K, Rautio P, Tuomi J (2000). A test of the compensatory continuum: fertilization increases and below-ground competition decreases the grazing tolerance of tall wormseed mustard ( Erysimum strictum). Evolutionary Ecology, 14, 353-372.
DOI URL |
[10] |
Lee JA (1998). Unintentional experiments with terrestrial ecosystems: ecological effects of sulphur and nitrogen pollutants. Journal of Ecology, 86, 1-12.
DOI URL |
[11] | Li ZH, Piippo S (1994). Preliminary list of bryophytes of Heishiding Nature Reserve, Guangdong Province, China. Tropical Bryology, 9, 35-41. |
[12] | Liu BY (刘滨扬), Liu WQ (刘蔚秋), Lei CY (雷纯义), Zhang YS (张以顺) (2009). Physiological responses of three bryophyte species of south China to simulated nitrogen deposition. Chinese Journal of Plant Ecology (植物生态学报), 33, 141-149. (in Chinese with English abstract) |
[13] | Liu WQ (刘蔚秋), Liu BY (刘滨扬), Wang J (王江), Lei CY (雷纯义) (2010). Responses of soil microbial communities to moss cover and nitrogen addition. Acta Ecologica Sinica (生态学报), 30, 1691-1698. (in Chinese with English abstract) |
[14] | Liu XE (刘雄恩), Wang BS (王伯荪) (1987). The vegetation classification system and main kinds of their distribution in Heishiding Nature Reserve. Ecological Science (生态科学), 1(2), 19-34. (in Chinese with English abstract) |
[15] |
Margolis HA, Waring RH (1986). Carbon and nitrogen allocation pattern of Douglas-fir seedlings fertilized with nitrogen in autumn. Canadian Journal of Forest Research, 16, 897-910.
DOI URL |
[16] |
Maschinski J, Whitham TG (1989). The continuum of plant responses to herbivory: the influence of plant association, nutrient availability and timing. The American Naturalist, 134, 1-19.
DOI URL |
[17] |
Nowak RS, Caldwell MM (1984). A test of compensatory photosynthesis in the field: implications for herbivory tolerance. Oecologia, 61, 311-318.
DOI URL PMID |
[18] |
Parker J (1962). Relationships among cold hardiness, water- soluble protein, anthocyanins, and free sugars in Hedera helix L. Plant Physiology, 37, 809-813.
DOI URL PMID |
[19] |
Pearce ISK, van der Wal R (2002). Effect of nitrogen deposition on growth and survival of montane Racomitrium lanuginosum health. Biological Conservation, 104, 83-89.
DOI URL |
[20] |
Pearce ISK, Woodin SJ, van der Wal R (2003). Physiological and growth responses of the montane bryophyte Racomitrium lanuginosum to atmospheric nitrogen deposition. New Phytologist, 160, 145-155.
DOI URL |
[21] |
Pearson J, Stewart GR (1993). The deposition of atmospheric ammonia and its effects on plants. New Phytologist, 125, 283-305.
DOI URL |
[22] |
Schnyder H, de Visser R (1999). Fluxes of reserve-derived and currently assimilated carbon and nitrogen in perennial ryegrass recovering from defoliation—the regrowing tiller and its component functionally distinct zones. Plant Physiology, 119, 1423-1436.
DOI URL PMID |
[23] | Soares A, Pearson J (1997). Short-term physiological responses of mosses to atmospheric ammonium and nitrate. Water, Air and Soil Pollution, 93, 225-254. |
[24] |
Thornton B, Millard P (1996). Nitrogen uptake by grasses: changes induced by competing neighbour plants differing in frequency of defoliation. Grass and Forage Science, 51, 242-249.
DOI URL |
[25] |
Tomassen HBM, Smolders AJP, Lamers LPM, Roelofs JGM (2003). Stimulated growth of Betula pubescens and Molinia caerulea on ombrotrophic bogs: role of high levels of atmospheric nitrogen deposition. Journal of Ecology, 91, 357-370.
DOI URL |
[26] |
Touchette BW, Burkholder JM (2007). Carbon and nitrogen metabolism in the seagrass, Zostera marina L.: environmental control of enzymes involved in carbon allocation and nitrogen assimilation. Journal of Experimental Marine Biology and Ecology, 350, 216-253.
DOI URL |
[27] | Vitousek PM, Aber JD, Howarth RW, Likens GE, Matson PA, Schindler DW, Schlesinger WH, Tilman DG (1997). Human alteration of the global nitrogen cycle: sources and consequences. Ecological Applications, 7, 737-750. |
[28] | Zhao SJ (赵世杰), Liu HS (刘华山), Dong XC (董新纯) (1998). Experimental Guidance for Plant Physiology (植物生理学实验指导). Chinese Agricultural Science and Technology Press, Beijing. (in Chinese) |
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