低温胁迫对不同母树年龄柠条锦鸡儿种子萌发幼苗生理和生长的影响

doi:10.17521/cjpe.2022.0210

植物生态学报 ›› 2024, Vol. 48 ›› Issue (2): 201-214.DOI: 10.17521/cjpe.2022.0210

低温胁迫对不同母树年龄柠条锦鸡儿种子萌发幼苗生理和生长的影响

高敏¹, 缑倩倩¹^,²^,^*(), 王国华¹^,²^,³, 郭文婷¹, 张宇¹, 张妍¹

¹山西师范大学地理科学学院, 太原 030031
²中国科学院西北生态环境资源研究院临泽内陆河流域研究站, 兰州 730010
³中国科学院西北生态环境资源研究院沙漠与沙漠化重点实验室, 兰州 730010

收稿日期:2022-05-20 接受日期:2023-05-16 出版日期:2024-02-28 发布日期:2023-06-08
通讯作者: 缑倩倩
基金资助:
国家自然科学基金(41807518);国家自然科学基金(42171033)

Effects of low temperature stress on the physiology and growth of Caragana korshinskii seedlings from different mother tree ages

GAO Min¹, GOU Qian-Qian¹^,²^,^*(), WANG Guo-Hua¹^,²^,³, GUO Wen-Ting¹, ZHANG Yu¹, ZHANG Yan¹

¹College of Geographical Science, Shanxi Normal University, Taiyuan 030031, China
²Linze Inland River Basin Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730010, China
³Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730010, China

Received:2022-05-20 Accepted:2023-05-16 Online:2024-02-28 Published:2023-06-08
Contact: GOU Qian-Qian
Supported by:
National Natural Science Foundation of China(41807518);National Natural Science Foundation of China(42171033)

摘要/Abstract

摘要：

为探讨母树年龄、低温胁迫及其交互作用对柠条锦鸡儿(Caragana korshinskii)幼苗叶片生理和生长的影响, 该研究选取晋西北丘陵风沙区5种母树年龄(7、13、19、41和51 a)人工林柠条锦鸡儿的种子进行萌发, 取2个月左右幼苗为实验材料, 设置低温((-8 ± 1) ℃)胁迫, 进行不同时长低温处理: W1 (6 h)、W2 (12 h)、W3 (24 h)、W4 (48 h)和W5 (72 h), 并以常规温度(25 ℃)培养为对照, 测定幼苗与抗冻性相关的生理和生长指标, 并采用隶属函数法进行综合评价。研究结果表明: (1)低温胁迫对不同母树年龄柠条锦鸡儿种子源萌发幼苗光合作用色素和渗透调节物质的影响存在显著差异, 其中母树年龄和低温胁迫对幼苗叶片叶绿素(Chl) a含量有显著影响, 母树年龄、低温胁迫及其交互作用对柠条锦鸡儿幼苗的Chl b、Chl (a+b)、类胡萝卜素含量和Chl a/b均有显著影响; 低温胁迫和母树年龄与低温胁迫的交互作用对可溶性糖含量有显著影响, 母树年龄、低温及其交互作用对游离脯氨酸含量有显著影响。随着低温胁迫时间的增长, 柠条锦鸡儿幼苗叶片内Chl a、Chl b和Chl (a+b)含量整体呈先降后升的趋势, 而Chl a/b和类胡萝卜素含量呈先升后降的趋势; 游离脯氨酸含量则呈上升趋势。(2)低温胁迫对不同母树年龄柠条锦鸡儿种子源幼苗生长的影响差异显著, 一方面, 不同母树年龄种子萌发幼苗的株高、叶片数、干质量、主根长和根冠比均存在显著差异, 随母树年龄的增加呈先升后降的趋势; 另一方面, 低温胁迫对幼苗株高有显著抑制作用, 而母树年龄和低温胁迫的交互作用则对幼苗的上述生长参数没有显著影响。(3)通过聚类分析低温胁迫下柠条锦鸡儿幼苗的抗冻性生理指标综合评价值, 将5种幼苗的耐低温性划分为两大类: 第一类包括7、13 a母树种源幼苗, 耐低温性强; 第二类包括19、41、51 a母树种源幼苗, 耐低温性弱, 这表明低龄(7、13 a)母树柠条锦鸡儿种子萌发幼苗比高龄(19、41、51 a)母树的具有更强的抗冻性。综上, 生理指标Chl b含量、Chl (a+b)含量、类胡萝卜素含量、Chl a/b和生长指标株高、叶片数、干质量、主根长和根冠比可作为柠条锦鸡儿幼苗的抗冻性鉴定指标, 同时, 在人工促进柠条林天然更新时, 可以选用青壮年柠条锦鸡儿的种子进行幼苗培育, 进而提高人工林更新的成功率。

关键词: 晋西北丘陵风沙区, 柠条锦鸡儿, 幼苗, 低温胁迫, 渗透调节, 光合色素, 根冠比

Abstract:

Aims This study investigated the effects of mother tree age, low temperature stress, and their interaction on the growth and leaf physiological characteristics of Caragana korshinskii seedlings.

Methods In this study, seeds of C. korshinskii were selected from an artificial forest in the hilly and sandy area of northwest Shanxi Province, representing five mother tree ages (7, 13, 19, 41 and 51 a). These seeds were germinated, and the resulting seedlings (about 2 months) were used as test material. The low temperature stress was applied at (-8 ± 1) °C with varying stress levels: W1 (6 h), W2 (12 h), W3 (24 h), W4 (48 h) and W5 (72 h). A control group (CK) was maintained at 25 °C (normal temperature). Different growth and physiological indicators related to frost resistance were determined, and a comprehensive evaluation was conducted using the membership function method.

Important findings The results showed that: (1) There were significant differences in the impact of low temperature stress on photosynthetic pigments and osmotic adjustment substances in C. korshinskii seedlings from different mother tree ages. Notably, mother tree age and low temperature stress had significant effects on chlorophyll (Chl) a content, while the contents of Chl b, Chl (a+b), carotenoid and Chl a/b in C. korshinskii seedlings were influenced by mother tree age, low temperature stress and their interaction. Low temperature stress as well as the interaction between low temperature stress and mother tree ages, had a significant effect on soluble sugar content, while mother tree ages, low temperature stress and their interaction significantly influenced free proline content. With the increasing low temperature stress duration, the contents of Chl a, Chl b, and Chl (a+b) in the leaves of C. korshinskii seedlings initially decreased and later increased, while the Chl a/b and carotenoid content exhibited an initial increase followed by a decreased, and the content of free proline increased. (2) Low temperature stress had variable effects on the growth of C. korshinskiiseedlings from different mother tree ages. Plant height, leaf number, dry mass, main root length and root shoot ratio of seedlings germinated from seeds from different age plants displayed significant differences, with values initially increasing and then decreasing with the increase in mother tree age. However, low temperature stress significantly inhibited the plant height of the seedlings, with no significant interaction observed between mother tree age and low temperature stress on the aforementioned growth parameters. (3) Seedlings germinated from the seeds of young mothers (7, 13 a) showed stronger frost resistance than those from older mothers (19, 41, 51 a). In summary, physiological parameters including Chl b content, Chl (a+b) content, Chl a/b, carotenoid content and growth parameters such as plant height, leaf number, dry mass, main root length and root shoot ratio can serve as indicators for assessing frost resistance in C. korshinskii seedlings. Besides that, when promoting the natural regeneration of C. korshinskii forest through artificial means, selecting seeds from young and middle-aged C. korshinskii plants can enhance the success of regeneration.

Key words: windy and sandy hilly area in northwest Shanxi, Caragana korshinskii, seedling, low temperature stress, osmoregulation, photosynthetic pigment, root shoot ratio

高敏, 缑倩倩, 王国华, 郭文婷, 张宇, 张妍. 低温胁迫对不同母树年龄柠条锦鸡儿种子萌发幼苗生理和生长的影响. 植物生态学报, 2024, 48(2): 201-214. DOI: 10.17521/cjpe.2022.0210

GAO Min, GOU Qian-Qian, WANG Guo-Hua, GUO Wen-Ting, ZHANG Yu, ZHANG Yan. Effects of low temperature stress on the physiology and growth of Caragana korshinskii seedlings from different mother tree ages. Chinese Journal of Plant Ecology, 2024, 48(2): 201-214. DOI: 10.17521/cjpe.2022.0210

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图/表 10

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指标 Index	梯度 Gradient	叶绿素a含量 Chl a content (mg·g^-1)	叶绿素b含量 Chl b content (mg·g^-1)	总叶绿素含量 Chl (a+b) content (mg·g^-1)	叶绿素比 Chl a/b	类胡萝卜素含量 Carotenoids content (mg·g^-1)	可溶性糖含量 Soluble sugar content (mg·g^-1)	游离脯氨酸含量 Free proline content (mg·g^-1)	丙二醛含量 Malondialdehyde content (μmol·g^-1)
L	L1	1.700 ± 0.088^a	0.968 ± 0.059^a	2.668 ± 0.125^a	1.910 ± 0.186^c	0.414 ± 0.037^c	0.003 ± 0.000^a	0.673 ± 0.040^a	0.032 ± 0.002^a
	L2	1.700 ± 0.084^a	0.727 ± 0.068^b	2.426 ± 0.129^b	2.581 ± 0.184^b	0.494 ± 0.028^b	0.003 ± 0.000^a	0.648 ± 0.048^ab	0.032 ± 0.008^a
	L3	1.569 ± 0.082^ab	0.648 ± 0.106^c	2.217 ± 0.178^c	2.899 ± 0.182^a	0.469 ± 0.024^bc	0.003 ± 0.000^a	0.590 ± 0.041^bc	0.026 ± 0.008^a
	L4	1.526 ± 0.064^ab	0.641 ± 0.099^c	2.167 ± 0.157^c	1.874 ± 0.077^c	1.033 ± 0.090^a	0.003 ± 0.000^a	0.552 ± 0.021^c	0.027 ± 0.002^a
	L5	1.482 ± 0.074^b	0.634 ± 0.095^c	2.117 ± 0.159^c	2.804 ± 0.218^a	0.439 ± 0.019^bc	0.003 ± 0.000^a	0.513 ± 0.017^c	0.028 ± 0.002^a
W	CK	2.068 ± 0.067^a	1.409 ± 0.051^a	3.476 ± 0.088^a	1.494 ± 0.072^d	0.367 ± 0.043^d	0.003 4 ± 0.000 0^b	0.547 ± 0.036^bc	0.035 ± 0.002^a
	W1	1.625 ± 0.037^b	0.469 ± 0.017^d	2.094 ± 0.049^b	3.259 ± 0.183^a	0.683 ± 0.074^a	0.003 5 ± 0.000 0^a	0.549 ± 0.042^bc	0.027 ± 0.001^a
	W2	1.360 ± 0.071^c	0.656 ± 0.079^b	2.016 ± 0.135^b	2.208 ± 0.190^c	0.543 ± 0.079^c	0.003 5 ± 0.000 0^a	0.532 ± 0.033^c	0.024 ± 0.002^a
	W3	1.545 ± 0.069^bc	0.599 ± 0.070^bc	2.144 ± 0.124^b	2.596 ± 0.199^b	0.619 ± 0.090^ab	0.003 5 ± 0.000 0^a	0.636 ± 0.039^ab	0.029 ± 0.002^a
	W4	1.432 ± 0.075^c	0.567 ± 0.060^c	2.000 ± 0.109^b	2.515 ± 0.204^b	0.569 ± 0.060^bc	0.003 4 ± 0.000 0^b	0.646 ± 0.033^a	0.030 ± 0.011^a
	W5	1.543 ± 0.071^bc	0.641 ± 0.058^b	2.184 ± 0.106^b	2.408 ± 0.186^bc	0.636 ± 0.100^a	0.003 4 ± 0.000 0^b	0.632 ± 0.055^ab	0.030 ± 0.002^a
F	L	3.086^**	56.385^**	9.640^**	50.915^**	161.596^**	1.032	6.192^**	0.375
	W	16.050^**	274.704^**	50.649^**	58.151^**	24.314^**	5.103^**	2.838^**	0.504
	L × W	1.166	20.123^**	2.324^**	10.380^**	13.488^**	1.960^**	3.856^**	0.145

指标 Index	梯度 Gradient	叶绿素a含量 Chl a content (mg·g^-1)	叶绿素b含量 Chl b content (mg·g^-1)	总叶绿素含量 Chl (a+b) content (mg·g^-1)	叶绿素比 Chl a/b	类胡萝卜素含量 Carotenoids content (mg·g^-1)	可溶性糖含量 Soluble sugar content (mg·g^-1)	游离脯氨酸含量 Free proline content (mg·g^-1)	丙二醛含量 Malondialdehyde content (μmol·g^-1)
L	L1	1.700 ± 0.088^a	0.968 ± 0.059^a	2.668 ± 0.125^a	1.910 ± 0.186^c	0.414 ± 0.037^c	0.003 ± 0.000^a	0.673 ± 0.040^a	0.032 ± 0.002^a
	L2	1.700 ± 0.084^a	0.727 ± 0.068^b	2.426 ± 0.129^b	2.581 ± 0.184^b	0.494 ± 0.028^b	0.003 ± 0.000^a	0.648 ± 0.048^ab	0.032 ± 0.008^a
	L3	1.569 ± 0.082^ab	0.648 ± 0.106^c	2.217 ± 0.178^c	2.899 ± 0.182^a	0.469 ± 0.024^bc	0.003 ± 0.000^a	0.590 ± 0.041^bc	0.026 ± 0.008^a
	L4	1.526 ± 0.064^ab	0.641 ± 0.099^c	2.167 ± 0.157^c	1.874 ± 0.077^c	1.033 ± 0.090^a	0.003 ± 0.000^a	0.552 ± 0.021^c	0.027 ± 0.002^a
	L5	1.482 ± 0.074^b	0.634 ± 0.095^c	2.117 ± 0.159^c	2.804 ± 0.218^a	0.439 ± 0.019^bc	0.003 ± 0.000^a	0.513 ± 0.017^c	0.028 ± 0.002^a
W	CK	2.068 ± 0.067^a	1.409 ± 0.051^a	3.476 ± 0.088^a	1.494 ± 0.072^d	0.367 ± 0.043^d	0.003 4 ± 0.000 0^b	0.547 ± 0.036^bc	0.035 ± 0.002^a
	W1	1.625 ± 0.037^b	0.469 ± 0.017^d	2.094 ± 0.049^b	3.259 ± 0.183^a	0.683 ± 0.074^a	0.003 5 ± 0.000 0^a	0.549 ± 0.042^bc	0.027 ± 0.001^a
	W2	1.360 ± 0.071^c	0.656 ± 0.079^b	2.016 ± 0.135^b	2.208 ± 0.190^c	0.543 ± 0.079^c	0.003 5 ± 0.000 0^a	0.532 ± 0.033^c	0.024 ± 0.002^a
	W3	1.545 ± 0.069^bc	0.599 ± 0.070^bc	2.144 ± 0.124^b	2.596 ± 0.199^b	0.619 ± 0.090^ab	0.003 5 ± 0.000 0^a	0.636 ± 0.039^ab	0.029 ± 0.002^a
	W4	1.432 ± 0.075^c	0.567 ± 0.060^c	2.000 ± 0.109^b	2.515 ± 0.204^b	0.569 ± 0.060^bc	0.003 4 ± 0.000 0^b	0.646 ± 0.033^a	0.030 ± 0.011^a
	W5	1.543 ± 0.071^bc	0.641 ± 0.058^b	2.184 ± 0.106^b	2.408 ± 0.186^bc	0.636 ± 0.100^a	0.003 4 ± 0.000 0^b	0.632 ± 0.055^ab	0.030 ± 0.002^a
F	L	3.086^**	56.385^**	9.640^**	50.915^**	161.596^**	1.032	6.192^**	0.375
	W	16.050^**	274.704^**	50.649^**	58.151^**	24.314^**	5.103^**	2.838^**	0.504
	L × W	1.166	20.123^**	2.324^**	10.380^**	13.488^**	1.960^**	3.856^**	0.145

生长参数 Growth factor	母树年龄 Mother tree age		低温胁迫 Low temperature stress		母树年龄×低温胁迫 Mother tree age × low temperature stress
生长参数 Growth factor	F	p	F	p	F	p
株高 Plant height (cm)	42.297	0.000	2.406	0.039	0.000	1
主根长 Taproot length (cm)	57.832	0.000	2.065	0.730	0.000	1
叶片数 Number of blades	23.410	0.000	2.826	0.533	0.026	1
干质量 Dry mass (g·plant^-1)	46.560	0.000	0.253	0.938	0.002	1
根茎比 Root stem ratio	51.376	0.000	1.790	0.118	0.062	1
根冠比 Root shoot ratio	12.779	0.000	0.047	0.999	0.005	1

生长参数 Growth factor	母树年龄 Mother tree age		低温胁迫 Low temperature stress		母树年龄×低温胁迫 Mother tree age × low temperature stress
生长参数 Growth factor	F	p	F	p	F	p
株高 Plant height (cm)	42.297	0.000	2.406	0.039	0.000	1
主根长 Taproot length (cm)	57.832	0.000	2.065	0.730	0.000	1
叶片数 Number of blades	23.410	0.000	2.826	0.533	0.026	1
干质量 Dry mass (g·plant^-1)	46.560	0.000	0.253	0.938	0.002	1
根茎比 Root stem ratio	51.376	0.000	1.790	0.118	0.062	1
根冠比 Root shoot ratio	12.779	0.000	0.047	0.999	0.005	1

母树年龄 Mother tree age (a)	低温胁迫 Low tempera- ture stress	株高 Plant height (cm)	主根长 Taproot length (cm)	叶片数 Number of blades	干质量 Dry mass (g·plant^-1)	根茎比 Root stem ratio	根冠比 Root shoot ratio
7	CK	16.71 ± 1.63^Ab	19.68 ± 1.42^Abc	49.00 ± 1.26^Ac	0.13 ± 0.01^Ac	1.24 ± 0.15^Aab	0.39 ± 0.01^Bab
	W1	15.00 ± 1.47^Ab	18.70 ± 1.35^Abc	48.00 ± 1.35^ABc	0.13 ± 0.01^Ac	1.49 ± 0.21^Aab	0.42 ± 0.01^Aab
	W2	14.43 ± 1.53^Ab	18.92 ± 1.46^Abc	47.00 ± 2.26^ABc	0.12 ± 0.01^Ac	1.49 ± 0.22^Aab	0.41 ± 0.01^ABab
	W3	13.87 ± 1.66^Ab	19.39 ± 2.03^Abc	46.00 ± 1.24^ABc	0.12 ± 0.01^Ac	1.50 ± 0.20^Aab	0.40 ± 0.01^ABab
	W4	13.60 ± 1.75^Ab	20.26 ± 1.55^Abc	45.00 ± 2.34^ABc	0.12 ± 0.01^Ac	1.49 ± 0.17^Aab	0.40 ± 0.01^ABab
	W5	13.45 ± 1.58^Ab	21.15 ± 1.45^Abc	44.00 ± 1.36^Bc	0.16 ± 0.03^Ac	1.49 ± 0.16^Aab	0.39 ± 0.01^Bab
13	CK	28.40 ± 2.98^Aa	15.78 ± 1.27^Ac	72.00 ± 7.68^Aa	0.29 ± 0.03^Aa	0.60 ± 0.10^Ac	0.19 ± 0.01^Ab
	W1	26.68 ± 2.44^Aa	14.73 ± 1.22^Ac	70.00 ± 6.72^Aa	0.28 ± 0.03^Aa	0.65 ± 0.13^Ac	0.22 ± 0.01^Ab
	W2	26.13 ± 2.65^Aa	14.95 ± 1.46^Ac	68.00 ± 7.43^Aa	0.27 ± 0.03^Aa	0.65 ± 0.13^Ac	0.20 ± 0.01^Ab
	W3	25.56 ± 2.78^Aa	15.40 ± 2.26^Ac	67.00 ± 7.72^Aa	0.27 ± 0.03^Aa	0.66 ± 0.13^Ac	0.20 ± 0.01^Ab
	W4	25.28 ± 3.15^Aa	16.28 ± 1.58^Ac	66.00 ± 6.55^Aa	0.27 ± 0.03^Aa	0.68 ± 0.13^Ac	0.19 ± 0.01^Ab
	W5	25.14 ± 2.66^Aa	17.17 ± 1.33^Ac	65.00 ± 4.72^Aa	0.26 ± 0.03^Aa	0.70 ± 0.12^Ac	0.19 ± 0.01^Ab
19	CK	18.89 ± 1.46^Ab	27.18 ± 2.02^Aa	64.83 ± 4.08^Aab	0.25 ± 0.02^Aa	1.46 ± 0.13^Aa	0.43 ± 0.10^Aa
	W1	17.17 ± 1.55^Ab	26.19 ± 2.44^Aa	63.83 ± 3.37^Aab	0.24 ± 0.02^Aa	1.72 ± 0.17^Aa	0.43 ± 0.10^Aa
	W2	16.62 ± 2.34^Ab	26.42 ± 2.03^Aa	62.83 ± 3.28^Aab	0.24 ± 0.02^Aa	1.72 ± 0.17^Aa	0.43 ± 0.10^Aa
	W3	16.05 ± 1.41^Ab	26.87 ± 2.35^Aa	61.83 ± 3.45^Aab	0.24 ± 0.02^Aa	1.72 ± 0.17^Aa	0.43 ± 0.10^Aa
	W4	15.77 ± 1.98^Ab	27.75 ± 1.77^Aa	60.83 ± 3.01^Aab	0.24 ± 0.02^Aa	1.71 ± 0.16^Aa	0.45 ± 0.09^Aa
	W5	15.63 ± 1.43^Ab	28.65 ± 2.33^Aa	59.83 ± 3.22^Aab	0.23 ± 0.02^Aa	1.71 ± 0.15^Aa	0.43 ± 0.10^Aa
41	CK	18.54 ± 0.58^Ab	23.17 ± 2.33^Aab	58.58 ± 2.49^Abc	0.22 ± 0.02^Aab	1.25 ± 0.06^Aab	0.44 ± 0.12^Aa
	W1	16.83 ± 0.65^ABb	22.17 ± 1.56^Aab	57.58 ± 3.45^Aabc	0.22 ± 0.02^Aab	1.46 ± 0.07^Aab	0.44 ± 0.12^Aa
	W2	16.38 ± 0.73^Bb	22.40 ± 1.44^Aab	56.58 ± 2.28^Aabc	0.21 ± 0.02^Aab	1.46 ± 0.07^Aab	0.44 ± 0.12^Aa
	W3	15.71 ± 0.65^Bb	22.85 ± 1.39^Aab	55.58 ± 2.33^Aabc	0.21 ± 0.02^Aab	1.46 ± 0.07^Aab	0.44 ± 0.12^Aa
	W4	15.43 ± 0.99^Bb	23.73 ± 2.06^Aab	55.58 ± 2.28^Aabc	0.21 ± 0.02^Aab	1.46 ± 0.07^Aab	0.44 ± 0.12^Aa
	W5	15.28 ± 0.78^Bb	24.63 ± 1.07^Aab	54.58 ± 1.96^Aabc	0.21 ± 0.02^Aab	1.47 ± 0.07^Aab	0.44 ± 0.12^Aa
51	CK	18.35 ± 1.23^Ab	18.82 ± 1.08^Ac	53.33 ± 3.77^Abc	0.17 ± 0.02^Abc	1.04 ± 0.06^Ab	0.30 ± 0.04^Aab
	W1	16.63 ± 1.16^Ab	17.81 ± 2.09^Ac	52.33 ± 3.37^Abc	0.17 ± 0.02^Abc	1.20 ± 0.08^Ab	0.29 ± 0.04^Aab
	W2	16.07 ± 1.26^Ab	18.04 ± 1.08^Ac	51.33 ± 4.22^Abc	0.17 ± 0.02^Abc	1.20 ± 0.08^Ab	0.30 ± 0.04^Aab
	W3	15.52 ± 1.44^Ab	18.50 ± 1.46^Ac	52.33 ± 3.78^Abc	0.16 ± 0.02^Abc	1.21 ± 0.08^Ab	0.30 ± 0.04^Aab
	W4	15.24 ± 1.35^Ab	19.37 ± 1.37^Ac	51.33 ± 3.26^Abc	0.16 ± 0.02^Abc	1.22 ± 0.08^Ab	0.30 ± 0.03^Aab
	W5	15.09 ± 1.22^Ab	20.28 ± 1.28^Ac	50.33 ± 3.66^Abc	0.16 ± 0.02^Abc	1.24 ± 0.07^Ab	0.29 ± 0.04^Aab

低温胁迫对不同母树年龄柠条锦鸡儿种子萌发幼苗生理和生长的影响

Effects of low temperature stress on the physiology and growth of Caragana korshinskii seedlings from different mother tree ages

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生理指标 Physiological index		光合指标 Photosynthetic index					渗透调节物质 Osmoregulation substance
生理指标 Physiological index		叶绿素a含量 Chl a content	叶绿素b含量 Chl b content	叶绿素比 Chl a/b	总叶绿素含量 Chl (a+b) content	类胡萝卜素含量 Carotenoids content	可溶性糖含量 Soluble sugar content	游离脯氨酸含量 Free proline content
光合指标 Photosynthetic index	叶绿素b含量 Chl b content	0.650^**
	叶绿素比 Chl a/b	-0.179	-0.731^**
	总叶绿素含量 Chl (a+b) content	0.896^**	0.920^**	-0.520^**
	类胡萝卜素含量 Carotenoids content	-0.102	-0.468^**	0.063	-0.326^**
渗透调节物质 Osmoregulation substance	可溶性糖含量 Soluble sugar content	-0.170	-0.318^**	0.308^**	-0.274^**	0.129
渗透调节物质 Osmoregulation substance	游离脯氨酸含量 Free proline content	0.073	-0.025	0.066	0.023	-0.069	0.129
膜脂过氧化指标 Membranelipid peroxidation index	丙二醛含量 Malondialdehyde content	0.123	0.127	-0.049	0.138	-0.073	-0.241^*	0.026

指标 Index		生长指标 Growth index
指标 Index		株高 Plant height	主根长 Taproot length	叶片数 Number of blades	干质量 Dry mass	根茎比 Root stem ratio	根冠比 Root shoot ratio
生长指标 Growth index	主根长 Taproot length	-0.305^**
	叶片数 Number of blades	0.723^**	-0.082
	干质量 Dry mass	0.730^**	0.160	0.749^**
	根茎比 Root stem ratio	-0.771^**	0.741^**	-0.461^**	-0.288^**
	根冠比 Root shoot ratio	-0.307^**	0.127	0.020	-0.167	0.215^*
生理指标 Physiological index	叶绿素a含量 Chl a content	0.102	-0.058	-0.017	0.004	-0.079	-0.069
	叶绿素b含量 Chl b content	-0.073	-0.058	-0.213^*	-0.243^*	0.056	-0.104
	叶绿素比 Chl a/b	0.143	0.002	0.203	0.249^*	-0.124	-0.011
	总叶绿素含量 Chl (a+b) content	0.010	-0.064	-0.133	-0.140	-0.008	-0.096
	类胡萝卜素含量 Carotenoids content	-0.028	0.164	0.138	0.206	0.055	0.268^*
	可溶性糖含量 Soluble sugar content	0.029	-0.035	0.190	0.184	-0.038	0.095
	游离脯氨酸含量 Free proline content	0.095	-0.089	0.116	0.029	-0.041	-0.051
	丙二醛含量 Malondialdehyde content	-0.124	-0.063	-0.226^*	-0.205	0.018	-0.014

林龄 Stand age	隶属函数值 Subordinate function value								抗冻综合评价值 Integrated assessment value (D)	排序 Sort order
林龄 Stand age	叶绿素a Chl a	叶绿素b Chl b	叶绿素比 Chl a/b	总叶绿素 Chl (a+b)	类胡萝卜素 Car	可溶性糖 SS	游离脯氨酸 Pro	丙二醛 MDA	抗冻综合评价值 Integrated assessment value (D)	排序 Sort order
7	1.000	0.245	1.000	0.000	1.000	0.000	0.886	0.782	0.636	1
13	0.714	0.486	0.445	0.095	0.548	1.000	1.000	0.000	0.530	2
19	0.000	1.000	0.000	0.202	0.000	0.258	0.284	0.080	0.200	5
41	0.200	0.494	0.032	0.609	0.050	0.313	0.142	0.603	0.283	4
51	0.414	0.000	0.067	1.000	0.104	0.317	0.000	1.000	0.347	3
权重 Weight	0.104	0.101	0.167	0.132	0.152	0.119	0.118	0.107

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