基于温带针阔混交林植物-土壤反馈的Janzen- Connell假说检验

doi:10.17521/cjpe.2021.0194

摘要/Abstract

摘要：

目前对森林生物多样性维持机制的研究主要集中在检验地上生物的多度丰富度等内容, 物种之间的交互作用及地下真菌是否影响生物多样性的维持在很大程度上还是未知的。探讨土壤真菌的作用及植物-土壤反馈在植物生长过程中的作用有重要意义。该研究以吉林蛟河针阔混交林中4个常见树种为研究对象, 利用杀菌剂处理及不同树种幼苗的栽种控制实验来检验Janzen-Connell假说, 计算生物反馈及特异性反馈, 并依据高通量测序方法测定土壤真菌多样性, 检验不同处理组间差异性。利用方差分解分析(VPA)分析两种反馈的幼苗生物量与树种本身、真菌功能类型及土壤元素间的关系。结果表明, 土壤杀菌处理并未显著影响生物反馈, 同物种土壤的幼苗生物反馈未表现出显著的距离制约效应。异种物种土壤并未显著提高水曲柳(Fraxinus mandshurica)幼苗的特异性反馈。此外, VPA结果表明, 影响植物-土壤反馈最大的因素是物种本身特性, 土壤和真菌的影响大小因反馈类型而异。该研究揭示了温带针阔混交林中植物-土壤反馈的影响因素, 同时反映出温带森林不同树种的Janzen-Connell效应存在较大的变异性, 为更好地研究物种间相互作用与其内在调控机制奠定基础。

关键词: Janzen-Connell假说, 植物-土壤反馈, 距离制约, 针阔混交林, 幼苗, 土壤真菌

Abstract:

Aims Current research on the maintenance mechanisms of forest biodiversity mainly focuses on the stage of examining the abundance of aboveground organisms. However, many gaps remain in our knowledge of interactions between species and on whether the underground fungi affect the maintenance of biodiversity. This study explores the role of soil fungi in maintenance of biodiversity and the relationship between plant-soil feedbacks and plant growth. We selected four tree species in a broadleaved Korean pine forest in Northeast China, and used controlled seedling planting experiments to test the Janzen-Connell hypothesis.

Methods Experimental treatments included fungicide application and controlled seedling planting of four tree species (Pinus koraiensis, Fraxinus mandshurica, Phellodendron amurenseand Tilia amurensis) in Jiaohe forest farm, Jilin Province. Biotic and specific feedbacks were assessed using the fungicide treatment and the control seedling planting of different tree species. Soil fungal diversity was determined by high-throughput sequencing method and tested for differences among different treatments. The relationships of the two feedback types with tree species, functional types of fungi and soil were analyzed by variance partitioning analysis (VPA).

Important findings Fungicide treatment did not significantly affect the biotic feedback, and seedling biomass in conspecific soil did not show significant distance dependence. The specific feedbacks were not significantly improved by presence of different tree species. In addition, VPA showed that the most important factor affecting plant-soil feedbacks was inherent species characteristics, with the influence of soil and fungi varying with the feedback type. This study identified the factors influencing plant-soil feedbacks in mixed forest of temperate zone, and demonstrated the variability of Janzen-Connell hypothesis with temperate forest tree species, laying a foundation for better understanding the interactions between species and the underlying regulation mechanisms.

Key words: Janzen-Connell hypothesis, plant-soil feedback, distance dependence, coniferous and broadleaved mixed forest, seedling, soil fungi

秦江环, 张春雨, 赵秀海. 基于温带针阔混交林植物-土壤反馈的Janzen- Connell假说检验. 植物生态学报, 2022, 46(6): 624-631. DOI: 10.17521/cjpe.2021.0194

QIN Jiang-Huan, ZHANG Chun-Yu, ZHAO Xiu-Hai. Testing Janzen-Connell hypothesis based on plant-soil feedbacks in a temperate coniferous and broadleaved mixed forest. Chinese Journal of Plant Ecology, 2022, 46(6): 624-631. DOI: 10.17521/cjpe.2021.0194

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2021.0194

https://www.plant-ecology.com/CN/Y2022/V46/I6/624

图/表 5

图1 植物-土壤反馈实验设计示意图。

Fig. 1 Diagrammatic illustration of plant-soil feedback experimental design.

图2 不同距离下温带针阔混交林树种幼苗生长生物反馈的差异。图中数字为p值。

Fig. 2 Seedling growth variations of biotic feedback under different distances in a temperate coniferous and broadleaved mixed forest. Number in the figure is p value.

图3 温带针阔混交林特异性反馈的树种差异。图中数字为p值。

Fig. 3 Variations of specific feedback of tree species in a temperate coniferous and broadleaved mixed forest. Number in the figure is p value. PA, Phellodendron amurense; PK, Pinus koraiensis; TA, Tilia amurensis.

图4 不同处理下温带针阔混交林树种幼苗根际真菌功能群比较。图中数字为p值。

Fig. 4 Comparison of fungal guild types of seedling rhizosphere under different treatments in a temperate coniferous and broadleaved mixed forest. Number in the figure is p value.

图5 温带针阔混交林生物和特异性反馈与真菌和土壤关系的方差分解。

Fig. 5 Variation partitioning for relationships of biotic and specific feedbacks with fungi and soil in a temperate coniferous and broadleaved mixed forest.

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