辽宁省9种兰科植物根内与根际土壤中真菌群落结构的差异
- 1 沈阳农业大学生物科学技术学院, 沈阳 110161
2 辽宁省全球变化与生物入侵重点实验室, 沈阳 110161
收稿日期: 2019-03-12
录用日期: 2019-11-18
网络出版日期: 2020-01-03
基金资助
辽宁省自然科学基金(201602656)
Difference in fungal communities between in roots and in root-associated soil of nine orchids in Liaoning, China
- 1 College of Biological Technology, Shenyang Agricultural University, Shenyang 110161, China
2 Key Laboratory of Biological Invasions and Global Changes, Shenyang 110161, China
Received date: 2019-03-12
Accepted date: 2019-11-18
Online published: 2020-01-03
Supported by
Supported by the Natural Science Foundation of Liaoning Province(201602656)
摘要
兰科植物的生存及生长高度依赖其根中的共生真菌, 其中的菌根真菌更是对兰科植物的种子萌发与后续生长有着非常重要的作用, 研究兰科植物根中的真菌, 尤其是菌根真菌, 对兰科植物的保护有重要作用。该研究利用第二代测序技术, 对中国辽宁省境内的9种属于极小种群的兰科植物的根、根际土和根围土中的真菌群落和菌根真菌组成进行了研究。结果显示, 兰科植物根中的真菌群落和根际土、根围土中的真菌群落具有显著差异。兰科植物根中的总操作分类单元(OTU)数目远小于根际土和根围土中的总OTU数目。同时, 兰科植物根中菌根真菌的种类和丰度与根际土、根围土中菌根真菌的种类与丰度没有明显联系。FunGuild分析结果显示, 丛枝菌根真菌在根际土与根围土中的丰度非常高, 但在兰科植物的根中却数量极少。这些结果表明, 兰科植物根中的真菌群落与土壤中的真菌群落在一定程度上是相互独立的。
关键词： 兰科植物; 菌根真菌; 土壤; 内转录间隔区(ITS); 高通量测序
本文引用格式
蒋玉玲, 陈旭辉, 苗青, 曲波 . 辽宁省9种兰科植物根内与根际土壤中真菌群落结构的差异[J]. 植物生态学报, 2019 , 43(12) : 1079 -1090 . DOI: 10.17521/cjpe.2019.0055
Abstract
Aims Orchid plants generally grow better when they are mycorrhizal since mycorrhizal fungi are likely to assist in orchid seeds’ germination. However, there is little quantitative work on it. Thus we hope to better understand this mechanism to benefit the orchid plants protection.
Methods We studied nine small population species of orchids grown in Liaoning Province, China. We analyzed the composition of orchid mycorrhizal fungi (OMF) and fungal communities in the roots, in the rhizosphere soil as well as bulk soil, by taking advantage of the next generation sequencing technology.
Important findings Our study showed that there was a significant difference in fungal communities among in the roots, the rhizosphere soil and the bulk soil, especially in the total operational taxonomic unit (OTU) number. Although the OTU number was far smaller in the roots than in the rhizosphere soil and bulk soil, the species and abundances of OMF were less relative to each other. FunGuild, an indicator to predict the functional fungi, indicated that Arbuscular Mycorrhizal fungi were abundence in the rhizosphere while were rare in the roots of orchids. In general, the fungal communities in the roots were not tightly correlated with that in the root-associated soil.
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