基于多源数据的新疆沙漠植物白梭梭遗传格局与扩散路径模拟
- 1石河子大学理学院, 干旱区景观生态重点实验室, 绿洲城镇与山盆系统生态兵团重点实验室, 新疆石河子 832000
2石河子大学生命科学学院, 绿洲城镇与山盆系统生态兵团重点实验室, 新疆石河子 832000
收稿日期: 2023-12-04
录用日期: 2024-05-22
网络出版日期: 2024-05-23
基金资助
国家自然科学基金(41561007)
Genetic pattern and diffusion path simulation of Haloxylon persicum in Xinjiang based on multi-source data
- 1Key Laboratory of Arid Land Landscape Ecology, Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology, College of Science, Shihezi University, Shihezi, Xinjiang 832000, China
2Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology, College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, China
Received date: 2023-12-04
Accepted date: 2024-05-22
Online published: 2024-05-23
Supported by
National Natural Science Foundation of China(41561007)
摘要
典型沙生植物白梭梭(Haloxylon persicum)在中国主要分布在新疆古尔班通古特沙漠, 随着第四纪沙漠形成、扩展发生过大范围的迁移扩张事件, 开展物种的遗传格局与迁移路径研究, 有助于深入理解其分布与进化。该研究利用白梭梭的自然分布数据与不同地理种群的trnS-trnG、trnV和ITS序列组合数据, 整合GIS空间分析、物种分布模型、GIS扩散路径分析、单倍型网络分析、分子方差分析等探究古尔班通古特沙漠12个自然分布种群的空间遗传变异格局, 利用最大熵模型(MaxEnt)模拟末次盛冰期和当前气候下的物种适宜分布格局, 分析冰后期的种群动态及其迁移扩散路径。主要结果有: (1) trnS-trnG和trnV组合序列比对长度为1 340 bp, 共定义9个叶绿体DNA (cpDNA)单倍型; ITS1-ITS4序列长576 bp, 共定义6个核糖体DNA (nrDNA)单倍型; (2)种群总的遗传多样性(HT)为0.862 (cpDNA)和0.777 (nrDNA), 显著高于种群内平均遗传多样性(HS) (0.155 (cpDNA)和0.217 (nrDNA)), 超过76%的遗传变异发生于采样的不同种群间; (3)分子错配分布曲线显示白梭梭存在近期种群扩张, 扩散路径分析结果显示末次盛冰期白梭梭主要沿古尔班通古特沙漠南缘发生由东向西的扩散; 在当前气候下, 仍然呈沿沙漠南缘向西扩散的路径, 但迁移的幅度明显降低。古尔班通古特沙漠白梭梭种群具有较高的遗传多样性, 种群间遗传分化显著, 末次盛冰期以来白梭梭显示出沿沙漠边缘迁移的扩散模式, 其中古尔班通古特沙漠南缘是白梭梭最重要的扩散路径, 是连接其东西种群的重要通道。
本文引用格式
马佳正 , 陈雨婷 , 马松梅 , 张丹 , 贺凌云 . 基于多源数据的新疆沙漠植物白梭梭遗传格局与扩散路径模拟[J]. 植物生态学报, 2024 , 48(10) : 1326 -1335 . DOI: 10.17521/cjpe.2023.0360
Abstract
Aims In China, the typical desert plant Haloxylon persicum is only distributed in Gurbantünggüt Desert, Xinjiang, China. With the formation and expansion of the Quaternary desert, extensive migration and expansion events occurred. The study of the genetic pattern and migration path of the species is helpful to further understand the distribution and evolution of H. persicum.
Methods In this study, the natural distribution data of H. persicum were combined with the trnS-trnG, trnV and ITS sequences of different geographic populations, GIS spatial analysis, species distribution modeling, GIS diffusion path analysis, haplotype network analysis (Network) and molecular analysis of variance (AMOVA) were integrated to investigate the spatial genetic variation pattern of 12 naturally distributed populations of H. persicum in Gurbantünggüt Desert, including 106 individuals. The Maximum Entropy Model (MaxEnt) was used to simulate the suitable distribution pattern of H. persicum in the Last Glacial Maximum and current climate. The analysis of population dynamics and dispersal paths during the late glacial period will help to understand the genetic variation pattern of H. persicum among different geographic populations in the Gurbantünggüt Desert.
Important findings The sequence length of trnS-trnG and trnV was 1 340 bp, and a total of 9 chloroplast DNA (cpDNA) haplotypes were defined. The sequence length of ITS1-ITS4 was 576 bp, and a total of 6 nuclear ribosomal DNA (nrDNA) haplotypes were defined. (2) The total genetic diversity (HT) of the population was 0.862 (cpDNA) and 0.777 (nrDNA), which was significantly higher than the average genetic diversity (HS) of the population (0.155 (cpDNA) and 0.217 (nrDNA)), and more than 76% of the genetic variation occurred in different sampled populations. (3) The mismatch distribution curve showed that H. persicum experienced recent population expansion, and the dispersal path analysis showed that H. persicumhad spread from west to east along the southern margin of Gurbantünggüt Desert since the Last Glacial Maximum, under the current climate period, H. persicum continues to spread westward along the southern margin of the desert, but the migration amplitude has obviously reduced. Haloxylon persicum population in Gurbantünggüt Desert has high genetic diversity and significant genetic differentiation among populations. Since the Last Glacial Maximum, H. persicum has exhibited a diffusion mode of migration along the edge of the desert. The southern edge of the Gurbantünggüt Desert is the most important diffusion path of Haloxylon persicum and an important channel connecting its east and west populations.
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