Abstract: In this paper, Fuzzy clustering analysis is used to study the scrub of Vitex negundo var. heterophylla. The procedure of this method is as follows:1. The calculation of similarity matrix R: This is the same as other approaches. Various similarity coefficients may be used here.2. The transformation of R to Fuzzy equivalent relation R*:Make R2,R4,R8, …, if RK=R2K △ R* then, R* is a fuzzy equivalent matrix.3. Clustering: select suitable values of λ and cluster accordingly. Establishing the similarity matrix R: let rjk=l-djk/M(j,k=l,2, 3, …41) where rjk is the similarity coefficient of both quadrants j and k; M, the proper constant chosen at will to make 0