植物生态学报 ›› 2012, Vol. 36 ›› Issue (9): 992-1003.DOI: 10.3724/SP.J.1258.2012.00992

• 研究论文 • 上一篇    下一篇

基于层次-向量法分析的普通鹿蹄草品质与生态因子的相关性

吕振江, 王冬梅, 李登武*()   

  1. 西北农林科技大学林学院, 陕西杨凌 712100
  • 收稿日期:2012-02-03 接受日期:2012-05-12 出版日期:2012-02-03 发布日期:2012-09-06
  • 通讯作者: 李登武
  • 作者简介: (E-mail: dengwuli@yahoo.com.cn)

Correlation between quality of Pyrola decorata and its ecological factors based on hierarchy- vector analysis

LÜ Zhen-Jiang, WANG Dong-Mei, LI Deng-Wu*()   

  1. College of Forestry, Northwest A & F University, Yangling, Shaanxi 712100, China
  • Received:2012-02-03 Accepted:2012-05-12 Online:2012-02-03 Published:2012-09-06
  • Contact: LI Deng-Wu

摘要:

采用层次-向量法, 从生态因子、有效成分含量、抗氧化活性3个层次, 分析了层次间关系, 研究了生态因子对普通鹿蹄草(Pyrola decorata)品质的影响, 揭示影响其有效成分含量的生态主导因子。结果表明: 不同地区普通鹿蹄草的4种有效成分含量及抗氧化活性差异显著。抗氧化活性(DPPHIC50值)与单宁、金丝桃苷、槲皮素含量呈负相关关系, 其相关系数分别为: -0.829、-0.378和-0.749, 与总黄酮含量表现为正相关(p = 0.260)。单宁含量和槲皮素含量是影响抗氧化活性的重要指标, 其含量增加时DPPHIC50值下降, 抗氧化活性增强。年平均气温、一月份平均气温、年积温、年极高气温、无霜期、土壤全氮、速效氮、速效磷和有机质是影响有效成分含量的主要因子, 其中土壤因子对有效成分含量的影响更显著。土壤因子对单宁和槲皮素含量的影响最大, 对金丝桃苷含量影响最小; 气候因子对4种有效成分含量影响较弱且对各成分的影响程度基本相同。研究结果表明, 选择合适的生境, 尤其是土壤因子可有效地提高普通鹿蹄草的品质。

关键词: 生态因子, 层次-向量分析法, 普通鹿蹄草

Abstract:

Aims Plant secondary metabolites have played a significant role in drug discovery and development. Their production and accumulation are affected by environmental factors. Our objective was to determine the dominant ecological factors influencing functional components content of Pyrola decorata, the relationships among ecological factors, the contents of functional components and antioxidant activity.
Method The HPLC method was used to determine the contents of tannin, quercetin and hyperoside. Total flavoniods content was evaluated by NaNO2-Al(NO3)3 spectrophotometric determination. The antioxidant activity was measured by DPPH radical assay in vitro. The hierarchy-vector analysis was applied to determine relationships between the quality of P. decorata and ecological factors.
Important findings The contents of functional components as well as the antioxidant activity in different regions were significantly different. Tannin, quercetin and hyperoside as the key factors affecting the antioxidant activity showed negative correlation with the antioxidant ability; their correlation coefficients were -0.829, -0.378 and -0.749, respectively. There was a positive correlation between DPPHIC50 value and the total flavoniods content (p = 0.260). DPPHIC50 values decreased while the functional components contents increased, which indicated the enhancement of antioxidant activity. Nine ecological factors contributed significantly to the functional components contents: annual mean air temperature, January mean air temperature, annual accumulated air temperature, annual extreme high air temperature, frost-free period, soil total nitrogen, available nitrogen, available phosphorus and organic matter. The soil factors made greater contributions to the contents of the four effective components than did climatic factors, and they also had a large impact on the content of tannin and quercetin compared with hyperoside. Therefore, selecting a suitable habitat, especially appropriate soil, could effectively improve the quality of P. decorata.

Key words: ecological factor, hierarchy-vector analysis, Pyrola decorata