植物生态学报 ›› 1991, Vol. 15 ›› Issue (3): 234-239.

所属专题: 红树林及红树植物

• 论文 • 上一篇    下一篇

红树植物秋茄和海莲幼苗过氧化物酶对栽培盐度条件的反应

郑文教,林鹏   

  • 发布日期:1991-03-10
  • 通讯作者: 郑文教

Peroxidase in two Mangrove Species, Kandelia candel and Bruguiera sexangula Seedlings, in Response to Variation of Salinity Condition

Zheng Wen-jiao, Lin Peng   

  • Published:1991-03-10
  • Contact: Su Zhi-xian

摘要: 本文探讨了系列海水盐度砂培的红树植物秋茄和海莲幼苗叶片、根尖的过氧化物酶活性及其同工酶对不同盐度条件的反应。结果表明:(1)秋茄苗:在低盐度0‰至10‰范围,叶过氧化物酶活性随盐度提高而略有增强,15‰以上则降低;根尖过氧化物酶活性则不同,随盐度(0—35‰)提高而降低。(2)海莲苗:随其生长基盐度(5—25‰)提高,叶过氧化物酶活性迅速降低,而根尖过氧化物酶活性在5‰至10‰盐度时略有提高,15‰以上迅速降低;而后高盐度(25一35‰)活性降低不明显。这表明,在盐度的影响下,秋茄苗过氧化物酶活性变化程度小而海莲大。(3)在同工酶谱表现上,两种植物幼苗(叶,根)均为主级酶带受盐度影响不明显,但次级酶带对盐度敏感。

Abstract: The activity and isozyme of peroxidase in leaf and root tip of the seedling of two mangrove species, Kandelia candeI and Bruguiera sexangula, cultivated in sand beds irrigated with seawates of different salinity were studied.The results showed that: (1) With increase in salinity, the activity of peroxidase in leaf of K. candel got enhanced in lower salinity (from 0 to 10) and decreased over 15‰ salinity, but the activity in root tip decreased as the salinity increased from 0‰ to 35‰. (2) The activity of peroxidase in leaf of B. sexangula declined with increasing salinity from 5‰ to 35‰, whereas that in root tip increased with salinity from 5‰ to 10‰, and began to decrease over 15. The decreasing trend slowed down in both leaf and root tip in the range of 25--35‰ salinity. (3) The peroxidase activity of Kandelia was less sensitive to salinity than that of Bruguiera. The results showed that peroxidase activity may be used as an indicator of salinity tolerance, ie. Kandelia can grow in higher salinity than Bruguiera. (4) Electrophoresis maps of peroxidase isozyme in two plants on polyacrylamide gel indicated that the main isozyme bands were more stable than the low-class isozyme bands in response to salinity.