收稿日期: 2008-11-27
修回日期: 2009-05-15
网络出版日期: 2009-09-30
基金资助
国家自然科学基金重点项目专题(30630015);华南植物园珠江三角洲城市生态与环境定位研究平台建设项目(200603)
EFFECTS OF IRRADIANCE AND SOIL MOISTURE ON ANTIOXIDANTS AND MEMBRANE LIPID PEROXIDATION PRODUCTS IN MIKANIA MICRANTHA AND CHROMOLAENA ODORATUM
Received date: 2008-11-27
Revised date: 2009-05-15
Online published: 2009-09-30
田间温室控制条件下研究入侵杂草薇甘菊(Mikania micrantha)和飞机草(Chromolaena odoratum)在不同光照(高、中、低光照)和土壤水分(高、中、低水分含量)交互作用下, 叶片超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性, 脯氨酸(Pro)、谷胱甘肽(GSH)以及膜脂过氧化物丙二醛(MDA)含量的变化, 探讨两种杂草对不同光照和土壤水分交互作用的生理适应性。结果表明: 高光下低水处理的两种杂草MDA含量均显著高于全水处理, 表现出明显的胁迫。此胁迫条件下薇甘菊通过迅速提高SOD和CAT活性, 提高Pro和GSH含量来清除活性氧, 维持代谢平衡, 但不能在干旱条件下维持较长时间; 飞机草的SOD活性、Pro和GSH含量也迅速提高, 在高光缺水的适应性响应中起了重要作用, CAT则未能发挥保护作用。相对于其他光照, 低光显著降低了薇甘菊的SOD、CAT活性和MDA含量, 而对飞机草上述指标的影响较小, 说明低光降低了两种杂草尤其是薇甘菊的抗氧化物代谢水平。
张玲玲, 孙芳芳, 温达志 . 薇甘菊和飞机草抗氧化物和膜脂过氧化物对光照和土壤水分的响应[J]. 植物生态学报, 2009 , 33(5) : 974 -983 . DOI: 10.3773/j.issn.1005-264x.2009.05.017
Aims Our objective was to better understand physiological acclimation to varying light and soil water conditions by established seedlings of Mikania micrantha and Chromolaena odoratum to provide insight into the control of these invasive species.
Methods Seedlings were grown under optimum light and water conditions for plant stability and then treated by three light (full, medium and low irradiance) and three soil water (full, medium and low water content) conditions in field glasshouses. We measured superoxide dismutase (SOD) and catalase (CAT) activities and proline (Pro), glutathione (GSH) and malondialdehyde (MDA) contents in leaves and compared the two species.
Important findings Under full irradiance, the MDA content of both species in the low soil water treatment was always significantly higher than in the full soil water treatment (p＜0.01), indicating that drought stressed both species under high light. Both species developed mechanisms to scavenge the active oxygen. M. micrantha improved the SOD, CAT activities and the Pro and GSH contents, though the antioxidants could not sustain for a long time; C. odoratum showed similar trends in the measured antioxidants, except CAT was less responsive and Pro and GSH played important roles in acclimation to high light and drought. Relative to other irradiances, low light significantly reduced SOD and CAT activities and MDA contents in M. micrantha, but had less effect on those variables in C. odoratum, implying that low light reduced the antioxidants metabolism of both species, especially M. micrantha.
Key words： Mikania micrantha; Chromolaena odoratum; antioxidants; SOD activities; MDA contents
| [1] | Asada K (1999). The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601-639. |
| [2] | Burke JJ, Hatfield JL (1985). Plant morphological and biochemical responses to field water deficits. III. Effect of foliage temperature and the potential activity of glutathione reductase. Plant Physiology, 79, 415-419. |
| [3] | Byers JE (2002). Physical habitat attribute mediates biotic resistance to non-indigenous species invasion. Oecologia, 130, 146-156. |
| [4] | Cock MJW (1982). Potential biological control agents for Mikania micrantha H.B.K. from the Neotropical region. Tropical Pest Management, 28, 242-254. |
| [5] | Ellman GL (1959). Tissue sulfhydryl groups. Archives Biochemistry and Biophysics, 82, 70-77. |
| [6] | Elton C (1958). The Ecology of Invasions by Animals and Plants. Methuen, London. |
| [7] | Fridovich I (1975). Superoxide dismutase. Annual Review of Biochemistry, 44, 147-159. |
| [8] | Gamble PE, Burke JJ (1984). Effect of water stress on the chloroplast antioxidant system. I. Alteration in glutathione reductase activity. Plant Physiology, 78, 615-621. |
| [9] | Giannopolitis CN, Ries SK (1977). Superoxide dismutase. II.Purication and quantitative relationship with water-soluble protein in seedlings. Plant Physiology, 59, 315-318. |
| [10] | Halliwell B, Chirico S (1993). Lipid peroxidation: its mechanism, measurement, and significance. American Journal of Clinical Neutralization, 57, 715-725. |
| [11] | Hao ZB (郝再彬), Cang J (苍晶), Xu Z (徐仲), Zhang D (张达) (2004). Plant Physiology Experiments (植物生理实验). Harbin Institute of Technology Press,Harbin. (in Chinese) |
| [12] | Hayashi F, Ichino T, Osanai M, Wada K (2000). Oscillation and regulation of proline content of P5CS and ProDH gene expressions in the light/dark cycles in Arabodopsis thaliana L. Plant Cell Physiology, 41, 1096-1101. |
| [13] | Janero DR (1990). Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine, 9, 515-540. |
| [14] | Li Y (李毅), Men Q (门旗), Luo Y (罗英) (2000). Effects of spatial variability of soil water on irrigation schedule. Agricultural Research in the Arid Areas (干旱地区农业研究), 18, 80-86. (in Chinese with English abstract) |
| [15] | Li ZG (李志刚), Zheng QE (郑启恩), Li H (黎桦), Li SL (李素丽) (2006). The characteristics of Eupatorium odoratum community in limestone mountain area of Long’an County in Guangxi. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 14, 196-201. (in Chinese with English abstract) |
| [16] | Lin R (林镕), Chen YL (陈艺林), Shi Z (石铸) (1985). Flora of China (Volume 74) (中国植物志第七十四卷). Science Press, Beijing, 56-58. (in Chinese) |
| [17] | Lin ZF (林植芳), Li SS (李双顺), Lin GZ (林桂珠), Sun GC (孙谷畴), Guo JY (郭俊彦) (1984). Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves. Acta Botanica Sinica (植物学报), 26, 605-615. (in Chinese with English abstract) |
| [18] | Lu P (鲁萍), Sang WG (桑卫国), Ma KP (马克平) (2006). Activity of antioxidant enzymes in the invasive plant under various environment stresses. Acta Ecologica Sinica (生态学报), 26, 3578-3583. (in Chinese with English abstract) |
| [19] | Liu X, Huang B (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science, 40, 503-513. |
| [20] | Mittler R (2002). Oxidative stress, antioxidants and stress tolerance. Trends of Plant Science, 7, 405-410. |
| [21] | Mishra NP, Fatma T, Singhal GS (1995). Development of antioxidative defense system of wheat seedlings in response to high light. Physiologia Plantarum, 95, 77-82. |
| [22] | Noctor G, Foyer CH (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249-279. |
| [23] | Quero JL, Villar R, Mrań?n T, Zamora R (2006). Interactions of drought and shade effects on seedlings of four Quercus species: physiological and structural leaf responses. New Phytologist, 170, 819-834. |
| [24] | Sakamoto A, Murata N (2002). The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant, Cell and Environment, 25, 163-171. |
| [25] | Smirnoff N (1993). The role of oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125, 27-58. |
| [26] | Tang ZC (汤章城) (1984). Accumulation of proline in plants under stressed conditions and its potential significance. Plant Physiology Communications (植物生理学通讯), 1, 15-21. (in Chinese) |
| [27] | Vanderwoude C, Scanlan JC, Davis B, Funkhouse S (2005). Plan for National Delimiting Survey for Siam Weed. Natural Resources and Mines Land Protection Services:Queensland Government. |
| [28] | Vitousek PM, D’Antonio CM, Loope LL, Westbrooks R (1996). Biological invasion as global environmental change. American Scientist, 84, 468-478. |
| [29] | Wang JF (王俊峰), Feng YL (冯玉龙) (2004). The effect of light intensity on biomass allocation, leaf morphology and relative growth rate of two invasive plants. Acta Phytoecologica Sinica (植物生态学报), 28, 781-786. (in Chinese with English abstract) |
| [30] | Wang Y (王胤), Yao RL (姚瑞玲) , Liang HK (梁厚宽), Nie JG (聂吉贵) , Yan PD (颜培栋), Yang ZQ (杨章旗) (2007). Effect of osmotic stress on MDA content and SOD activity in Cyclocarya paliurus. Guangxi Forestry Science (广西林业科学), 36, 133-136. (in Chinese with English abstract) |
| [31] | Wen DZ (温达志), Ye WH (叶万辉), Feng HL (冯惠玲), Cai CX (蔡楚雄) (2000). Comparison of basic photosynthetic characteristics between exotic invader weed Mikania micrantha and its companion species. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 8, 139-146. (in Chinese with English abstract) |
| [32] | Xie MJ (谢明吉), Ke WS (柯文山), Wang WX (王万贤), Xiong ZT (熊治廷), Wu MY (吴明煜), Chen JJ (陈建军) (2005). MDA accumulation and antioxidation capacity of two Elsholtzia splendens populations under copper stress. Chinese Journal of Ecology (生态学杂志), 24, 935-938. (in Chinese with English abstract) |
| [33] | Xie Y, Li Z, William PG, Li D (2000). Invasive species in China―an overview. Biodiversity Conservation, 10, 1317-1341. |
| [34] | Xu KY (许凯扬), Ye WH (叶万辉), Duan XW (段学武), Su XG (苏新国), Xu ZF (徐志防) (2004). Physiological adaptation of Alternanthera philoxeroides under water stress condition induced by polyethylene glycol. Journal of Zhejiang University (Agriculture and Life Sciences)(浙江大学学报(农业与生命科学版)), 30, 271-277. (in Chinese with English abstract) |
| [35] | Yang QH, Ye WH, Deng X, Cao HL, Zhang Y, Xu KY (2005). Seed germination ecophysiology of Mikania micrantha H.B.K. Botanical Bulletin of Academia Sinica, 46, 293-299. |
| [36] | Zan QJ (昝启杰), Wang YJ (王勇军), Wang BS (王伯荪), Liao WB (廖文波), Li MG (李鸣光) (2000). The distribution and harm of the exotic weed Mikania micrantha. Chinese Journal of Ecology (生态学杂志), 19, 58-61. (in Chinese with English abstract) |
| [37] | Zeng SX (曾韶西), Wang YR (王以柔), Liu HX (刘鸿先) (1991). Some enzymatic reactions related to chlorophyll degradation in cucumber cotyledons under chilling in the light. Acta Phytophysiologica Sinica (植物生理学报), 17, 177-182. (in Chinese with English abstract) |
| [38] | Zhang LL, Wen DZ (2009). Structural and physiological responses of two invasive weeds Mikania micrantha and Chromolaena odorata to contrasting light and soil water conditions. Journal of Plant Research, 122, 69-79. |
/
| 〈 |
|
〉 |
