植物生态学报 >

2018 , Vol. 42 >Issue 11: 1113 - 1119

DOI: https://doi.org/10.17521/cjpe.2018.0145

研究论文

刺槐木质部栓塞脆弱性检测的方法比较

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  • 1西北农林科技大学理学院, 陕西杨凌 712100
    2西北农林科技大学林学院, 陕西杨凌 712100

收稿日期: 2018-06-19

  录用日期: 2018-10-19

  网络出版日期: 2019-03-13

基金资助

国家自然科学基金(31201122);国家自然科学基金(31570588)

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Comparison of methods for detecting vulnerability of xylem embolism in Robinia pseudoacacia

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  • 1College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China;
    2College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China;

Received date: 2018-06-19

  Accepted date: 2018-10-19

  Online published: 2019-03-13

Supported by

Supported by the National Natural Science Foundation of China(31201122);Supported by the National Natural Science Foundation of China(31570588)

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摘要

在全球变暖的背景下, 植物木质部栓塞脆弱性是林木死亡率升高的重要生理学因素。然而不同方法在长导管树种上建立的栓塞脆弱性曲线存在较大差异。该研究以长导管树种刺槐(Robinia pseudoacacia)为研究对象, 利用自然干燥法、Cochard Cavitron离心机法以及Sperry离心机法建立了栓塞脆弱性曲线, 旨在探讨不同检测方法的合理性。在Sperry离心法中, 使用了两种规格的转子, 从而对“开口导管假象”学说进行了检验。研究结果表明: 自然干燥法建立的栓塞脆弱性曲线为“s”形, 而Cochard Cavitron离心机法和Sperry离心机法建立的栓塞脆弱性曲线为“r”形; 自然干燥法与离心机法建立的曲线存在显著性差异, 且两种离心机法建立的曲线也具有显著性差异。尽管刺槐枝条的导管长度分布表明14.4 cm长的刺槐枝条具有更高比例的开放导管, 但用Sperry离心机法在27.4 cm和14.4 cm长茎段上建立的栓塞脆弱性曲线相似, 表明Sperry离心机法检测刺槐脆弱性曲线时未产生“开口导管假象”, 具有更为可靠的检测结果。

关键词： 栓塞脆弱性; 脆弱性曲线; 自然干燥法; Cochard Cavitron离心机法; Sperry离心机法

本文引用格式

安瑞, 孟凤, 尹鹏先, 杜光源 . 刺槐木质部栓塞脆弱性检测的方法比较[J]. 植物生态学报, 2018 , 42(11) : 1113 -1119 . DOI: 10.17521/cjpe.2018.0145

Abstract

Aims The vulnerability of xylem embolism is one of the key physiological factors that are related to plant mortality. Vulnerability curves are typically used for determining the vulnerability of xylem embolism. However, the shapes of vulnerability curves vary with the methods of assessment, especially in plant species with long xylem vessels. This study aims to investigate the feasibility of using different methods for establishment of vulnerability curves.
Methods Robinia pseudoacacia branches, with long xylem vessels, were used as plant materials for comparison of three different methods in establishing vulnerability curves, including bench top dehydration, Cochard Cavitron centrifugation and Sperry centrifugation. In the Sperry centrifugation method, rotors of two different sizes were used to test the ‘open vessel artifact’ hypothesis.
Important findings The vulnerability curve established by the bench top dehydration method displayed an “s” shape, while both the Cochard Cavitron centrifugation and Sperry centrifugation methods produced “r” shape curves. Vulnerability curves derived from the bench top dehydration method and the centrifugation methods were significantly different. Using the Sperry centrifugation method, the R. pseudoacacia branch samples in the 14.4 cm rotor had a higher proportion of open vessels, while the embolic vulnerability curves established on the 27.4 cm and 14.4 cm long stem segments were similar, indicating that the Sperry centrifugation method does not produce “open vessel artifact”.

Key words： embolism vulnerability; vulnerability curve; bench top dehydration; Cochard Cavitron centrifugation; Sperry centrifugation

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