不同生长期樟子松外生菌根真菌群落物种组成及其驱动因素
收稿日期: 2022-11-28
录用日期: 2023-04-26
网络出版日期: 2023-05-04
基金资助
内蒙古自治区科技计划项目(2022YFHH0131);中央高校基本科研业务费项目(2021ZY47)
Species composition and driving factors of the ectomycorrhizal fungal community associated with Pinus sylvestris var. mongolica at different growth periods
Received date: 2022-11-28
Accepted date: 2023-04-26
Online published: 2023-05-04
Supported by
Science and Technology Project of Nei Mongol Autonomous Region(2022YFHH0131);Fundamental Research Funds for the Central Universities(2021ZY47)
阐明不同生长期樟子松(Pinus sylvestris var. mongolica)外生菌根真菌群落物种组成、分子生态网络特征及其驱动因子, 可为樟子松人工林可持续经营管理提供理论依据。该研究以毛乌素沙地不同树龄(23、33和44年)樟子松为研究对象, 采用野外调查取样、Illumina MiSeq技术和分子生态网络等生物信息学分析相结合的方法, 比较分析生长季初期(4月)、生长旺盛期(7月)和生长季末期(9月)外生菌根真菌群落物种组成、菌群间相互作用关系及群落变化主要影响因子。主要结果有: 1)生长期对樟子松外生菌根真菌丰富度和Chao1指数影响显著, 生长旺盛期Chao1指数和Simpson多样性指数显著高于其他两个生长期; 树龄对外生菌根真菌多样性没有显著影响。2)毛乌素沙地樟子松根尖共鉴定到外生菌根真菌2门4纲7目18科28属; 生长季初期、旺盛期和末期的优势类群分别为棉革菌属(Tomentella)、丝盖伞属(Inocybe)和地孔菌属(Geopora), 不同树龄优势类群均为地孔菌属; 优势类群棉革菌属和丝盖伞属为生长旺盛期和生长季末期的共有指示菌属。3)生长季末期外生菌根真菌网络节点数、边数最大, 其群落结构更加复杂, 菌种间存在较强的相互作用。4)土壤pH和年平均相对湿度是显著影响樟子松外生菌根真菌群落物种组成差异的环境因子。结论: 生长期和树龄均对樟子松外生菌根真菌多样性与群落物种组成产生影响, 其中生长期的作用强于树龄; 外生菌根真菌季节动态分布主要取决于土壤性质和气候条件。
任悦, 高广磊, 丁国栋, 张英, 赵珮杉, 柳叶 . 不同生长期樟子松外生菌根真菌群落物种组成及其驱动因素[J]. 植物生态学报, 2023 , 47(9) : 1298 -1309 . DOI: 10.17521/cjpe.2022.0480
Aims This study aimed to illustrate community structure, molecular ecological network and driving factors of ectomycorrhizal fungi associated with Pinus sylvestris var. mongolica at different growth periods, and provide a theoretical basis for sustainable management of P. sylvestris var. mongolica plantation.
Methods Pinus sylvestris var. mongolica with different tree ages (23, 33 and 44 a) in Mau Us Sandy Land were targeted. The species compositions, interaction and main drivers of ectomycorrhizal fungi at the early growth period (Apr.), vigorous growth period (July) and the late growth period (Sept.) were identified by field investigation and sampling, illumina high-throughput sequencing and molecular ecological network analysis, respectively.
Important findings 1) The growth period had significant effect on the richness and Chao1 index of ectomycorrhizal fungi, with significantly higher Chao1 and Simpson diversity index in vigorous growth period than in early and late growth periods. The tree age had no significant effect on the ectomycorrhizal fungal diversity index. 2) In Mau Us Sandy Land, the ectomycorrhizal fungi associated with P. sylvestris var. mongolica were identified into 2 phyla, 4 classes, 7 orders, 18 families, and 28 genera. The dominant genera were Tomentella, Inocybe and Geopora at the early, vigorous and late growth periods, respectively. The Tomentella and Inocybe were the common indicator genera in both vigorous and late growth periods. 3) At the end of the growing season, the nodes and edges of the ectomycorrhizal fungal networks were the largest, indicating that the ectomycorrhizal fungal community was more complex and there was strong interaction between fungal species. 4) Soil pH and annual relative humidity were the key environmental factors that significantly affected the community composition of ectomycorrhizal fungi. Our results demonstrated that the diversity and community composition of ectomycorrhizal fungi were affected by the growth stage and tree age, with a stronger effect in growth stage than in tree age. The seasonal dynamic distribution of ectomycorrhizal fungi mainly depended on soil properties and climate conditions.
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