植物生态学报 >

2025 , Vol. 49 >Issue 4: 638 - 652

DOI: https://doi.org/10.17521/cjpe.2024.0075

研究论文

生长调节剂对低温胁迫及复温下红海榄幼苗光合特性的影响

  • 欧阳子龙 ,
  • 贾湘璐 ,
  • 石景忠 ,
  • 滕维超 ,
  • 刘秀
展开
  • 1广西壮族自治区林业科学研究院, 南宁 530002
    2南宁植物园, 南宁 530002
    3南宁青秀山风景名胜旅游开发有限责任公司, 南宁 530004
    4广西大学林学院, 南宁 530004
* (xiuliu1010@126.com)

收稿日期: 2024-03-15

  录用日期: 2024-08-23

  网络出版日期: 2024-08-26

基金资助

广西重点研发计划(桂科AB24010288);广西林业科技推广示范项目(桂科[2021]1号)

收起

Effects of growth regulators on photosynthetic characteristics of Rhizophora stylosa seedlings under low temperature stress and re-warming

  • OUYANG Zi-Long ,
  • JIA Xiang-Lu ,
  • SHI Jing-Zhong ,
  • TENG Wei-Chao ,
  • LIU Xiu
Expand
  • 1Guangxi Forestry Research Institute, Nanning 530002, China
    2Nanning Botanical Garden, Nanning 530002, China
    3Nanning Qingxiu Mountain Scenic Spots Tourism Development Co., Ltd., Nanning 530004, China
    4College of Forestry, Guangxi University, Nanning 530004, China
* (xiuliu1010@126.com)

Received date: 2024-03-15

  Accepted date: 2024-08-23

  Online published: 2024-08-26

Supported by

Guangxi Key Research and Development Project(Guike AB24010288);Guangxi Forestry Science and Technology Promotion Demonstration Project(Guike [2021] 1)

Fold

摘要

红海榄(Rhizophora stylosa)是沿海植物生态系统的重要树种, 具有修复环境污染、稳固海岸、净化过滤水质等功能。低温胁迫是新时代育种工作面临的挑战之一, 低温通过限制红海榄幼苗的正常生长从而影响红海榄群落的更新和分布格局。生长调节剂能提高植物的抗逆性, 揭示生长调节剂对低温胁迫下红海榄光合特性的缓解作用, 能为红海榄苗期培育和低温防控提供一定的科学依据和理论指导。该研究以一年生和二年生红海榄幼苗为实验材料, 设置不同种类和浓度生长调节剂(6-BA、NAA、GA3、IAA)处理, 对低温胁迫和复温时期红海榄幼苗叶片光合色素(叶绿素a、叶绿素b、总叶绿素和类胡萝卜素)含量以及光合作用指标(净光合速率、气孔导度、胞间CO2浓度、蒸腾速率)进行比较, 探究生长调节剂对红海榄幼苗光合特性的影响。结果表明: 1)低温胁迫抑制了红海榄幼苗的光合作用, 使一、二年生红海榄光合色素含量和光合作用指标的值均显著降低。2)添加适当浓度生长调节剂缓解了低温的抑制作用, 同时促进了复温效果, 改善了红海榄的光合生理过程。3)过高浓度生长调节剂(150 mg·L-1 NAA)导致红海榄幼苗受低温胁迫程度加剧, 因此筛选适宜的生长调节剂种类和浓度极为重要。4)主成分分析表明, 二年生红海榄较一年生具有更强的低温抗逆性。5)隶属函数分析表明, 100和200 mg·L-1 GA3、150 mg·L-1 IAA、400 mg·L-1 6-BA、50 mg·L-1 NAA在抵御低温胁迫和复温过程中均具有较好的效果。

关键词： 生长调节剂; 红海榄; 光合特性; 低温胁迫; 复温; 缓解效应

本文引用格式

欧阳子龙 , 贾湘璐 , 石景忠 , 滕维超 , 刘秀 . 生长调节剂对低温胁迫及复温下红海榄幼苗光合特性的影响[J]. 植物生态学报, 2025 , 49(4) : 638 -652 . DOI: 10.17521/cjpe.2024.0075

Abstract

Aims Rhizophora stylosa plays a crucial role in coastal plant ecosystems, contributing to environmental pollution remediation, coastline stabilization, and water quality purification and filtration. Cold stress poses a significant challenge to breeding efforts in the modern era, impeding the normal growth of R. stylosa seedlings and consequently impacting the regeneration and distribution patterns of R. stylosa communities. Growth regulators can improve plant stress resistance. Revealing the alleviating effect of growth regulators on photosynthetic characteristics of R. stylosa under low temperature stress could provide a scientific basis and theoretical guidance for the cultivation, the low temperature prevention and control of R. stylosa.

Methods In this study, the effects of growth regulators on R. stylosa seedlings were investigated by comparing the photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids) content and photosynthetic parameters (net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration, transpiration rate) of R. stylosa seedlings under low temperature stress and rewarming.

Important findings 1) Low temperature stress inhibited the photosynthetic process of R. stylosa seedlings by significantly declining the photosynthetic pigment content and photosynthetic parameters. 2) Adding appropriate concentrations of growth regulators alleviated the low temperature inhibition, promoted the rewarming effect, and improved the photosynthetic process of R. stylosa. 3) Excessive concentrations of growth regulators (150 mg·L-1 NAA) led to the aggravation of low temperature stress in R. stylosa seedlings, which highlighted the extreme importance to screen appropriate species and concentrations. 4) Principal component analysis showed that biennial R. stylosa seedlings had stronger low temperature resistance than annual R. stylosa seedlings. 5) Combining with membership function analysis, 100 and 200 mg·L-1 GA3, 150 mg·L-1 IAA, 400 mg·L-1 6-BA, and 50 mg·L-1 NAA had better effects on R. stylosa seedlings under low temperature stress and rewarming.

Key words： growth regulator; Rhizophora stylosa; photosynthetic characteristics; low temperature stress; rewarming; mitigation effect

参考文献

[1] Altaf MA, Shu HY, Hao YY, Ali Mumtaz M, Lu X, Wang ZW (2022). Melatonin affects the photosynthetic performance of pepper (Capsicum annuum L.) seedlings under cold stress. Antioxidants, 11, 2414. DOI: 10.3390/antiox11122414.
[2] Battal P, Erez ME, Turker M, Berber I (2008). Molecular and physiological changes in maize (Zea mays L.) induced by exogenous NAA, ABA and MeJa during cold stress. Annales Botanici Fennici, 45, 173-185.
[3] Cai X, Zhang J, Zhang DJ, Guo JT, Meng PH (2021). The variation of photosynthetic pigment of ornamental kale in the growing period. Molecular Plant Breeding, 19, 7232-7239.
  [蔡霞, 张洁, 张德军, 郭惊涛, 孟平红 (2021). 观赏羽衣甘蓝生长期植物光合色素变化规律. 分子植物育种, 19, 7232-7239.]
[4] Chauhan J, Prathibha MD, Singh P, Choyal P, Mishra UN, Saha D, Kumar R, Anuragi H, Pandey S, Bose B, Mehta B, Dey P, Dwivedi KK, Gupta NK, Singhal RK (2023). Plant photosynthesis under abiotic stresses: damages, adaptive, and signaling mechanisms. Plant Stress, 10, 100296. DOI: 10.1016/j.stress.2023.100296.
[5] Fu H (2023). Effects of Eco-geographical Factors on Phenotypic Characters and Cold Resistance of Zanthoxylum Germplasm Resources in Introduced Areas. Master degree dissertation, Chongqing Three Gorges University, Chongqing.
  [付豪 (2023). 引种地生态地理因素对花椒种质资源表型性状及抗寒性的影响. 硕士学位论文, 重庆三峡学院, 重庆.]
[6] Gao JF (2006). Experimental Supervision of Plant Physiology. Higher Education Press, Beijing.
  [高俊凤 (2006). 植物生理学实验指导. 高等教育出版社, 北京.]
[7] Jia XL, Liu X, Ouyang ZL, Liu JZ, Fan DH, Teng WC (2022). Effects of growth regulators on growth and nutrients of Rhizophora stylosa seedlings. Guangxi Forestry Science, 51, 663-669.
  [贾湘璐, 刘秀, 欧阳子龙, 刘佳哲, 樊东函, 滕维超 (2022). 生长调节剂对红海榄幼苗生长及营养物质的影响. 广西林业科学, 51, 663-669.]
[8] Jin MR, Liu YL, Shi BS, Yuan H (2023). Exogenous IAA improves the seedling growth of Syringa villosa via regulating the endogenous hormones and enhancing the photosynthesis. Scientia Horticulturae, 30, 111585. DOI: 10.1016/j.scienta.2022.111585.
[9] Kudoh H, Sonoike K (2002). Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature. Planta, 215, 541-548.
[10] Li H, Wang JQ, Liu Q (2020). Photosynthesis product allocation and yield in sweet potato with spraying exogenous hormones under drought stress. Journal of Plant Physiology, 253, 153265. DOI: 10.1016/j.jplph.2020.153265.
[11] Li JX, Bai XM, Ran F, Zhang CZ, Yan YB, Li P, Chen H (2024). Effects of combined extreme cold and drought stress on growth, photosynthesis, and physiological characteristics of cool-season grasses. Scientific Reports, 14, 116. DOI: 10.1038/s41598-023-49531-1.
[12] Li TZ, Zheng DF, Feng NJ, Zhao LM, Mu DW, Liu L, Chen GJ, Zhou X (2022). Effects of plant growth regulators on physiological characteristics and yield of litchi plants under low temperature stress. Chinese Journal of Tropical Crops, 43, 509-519.
  [李甜子, 郑殿峰, 冯乃杰, 赵黎明, 母德伟, 刘玲, 陈观杰, 周行 (2022). 生长调节剂对低温胁迫下荔枝生理特性及产量的影响. 热带作物学报, 43, 509-519.]
[13] Li XN, Jiang HD, Liu FL, Cai J, Dai TB, Cao WX, Jiang D (2013). Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin. Plant Growth Regulation, 71, 31-40.
[14] Liao YQ (2020). Research and Analysis of High Resolution Remote Sensing Monitoring and Characteristic of Mangrove in Huidong. Master degree dissertation, Hunan University of Science and Technology, Xiangtan, Hunan.
  [廖亚琴 (2020). 惠东红树林的高分辨率遥感监测与特征分析研究. 硕士学位论文, 湖南科技大学, 湖南湘潭.]
[15] Lin X (2010). Response of Germination and Growth in Ficus concinna var. subsessilis to Some Ecological Factors. PhD dissertation, Beijing Forestry University, Beijing.
  [林霞 (2010). 无柄小叶榕发芽与生长对不同生态因子的响应研究. 博士学位论文, 北京林业大学, 北京.]
[16] Lü MM, Wu YH, Ma ZH, Chen Y, Lin SZ (2021). Effects of growth regulators on growth and photosynthetic characteristics of Chinese fir seedlings under aluminum stress. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 50(1), 69-78.
  [吕蒙蒙, 武忆寒, 马志慧, 陈宇, 林思祖 (2021). 铝胁迫下生长调节剂对杉木幼苗生长及光合特性的影响. 福建农林大学学报(自然科学版), 50(1), 69-78.]
[17] Luo DY, Zhang XH, Li QL, Wang J, Zheng S, Zhang TG (2020). Regulation of α-naphthaleneacetic acid on cold resistance of Brassica campestris seedlings under low temperature stress. Chinese Journal of Ecology, 39, 99-109.
  [罗丹瑜, 张小花, 李巧丽, 王娟, 郑晟, 张腾国 (2020). α-萘乙酸对低温胁迫下油菜幼苗抗寒性的影响. 生态学杂志, 39, 99-109.]
[18] Ma D (2011). Research on Cold Resistance of Clematis heracleifoli in Harbin. Master degree dissertation, Northeast Forestry University, Harbin.
  [马迪 (2011). 哈尔滨地区大叶铁线莲抗寒性的研究. 硕士学位论文, 东北林业大学, 哈尔滨.]
[19] Miranda JC, López de Heredia U, Fernández V, Pita P (2023). Phenotypic plasticity in Pinus canariensis seedlings growing at chilling and freezing temperatures. Frontiers in Forests and Global Change, 6, 1303886. DOI: 10.3389/ffgc.2023.1303886.
[20] Mohamed E, Otmani, Coggins C Jr, Agusti M, Lovatt C (2000). Plant growth regulators in citriculture: world current uses. Critical Reviews in Plant Sciences, 19, 395-447.
[21] Nishijima T (2023). Use of plant growth regulators for floriculture in Japan. Scientia Horticulturae, 309, 111630. DOI: 10.1016/j.scienta.2022.111630.
[22] Ouyang ZY, Chen WB, Ouyang SL, Li ZH, He YJ, Yan LH, Jiang LY, Tian XM, Xiang GF (2021). Effects of low phosphorus stress on physiological characteristics of Cyclobalanopsis gilva seedling leaves. Journal of Central South University of Forestry & Technology, 41(1), 69-79.
  [欧阳泽怡, 陈雯彬, 欧阳硕龙, 李志辉, 何友军, 颜立红, 蒋俐媛, 田晓明, 向光锋 (2021). 低磷胁迫对赤皮青冈幼苗叶片生理指标的影响. 中南林业科技大学学报, 41(1), 69-79.]
[23] Ruan ZP (2012). Comprehensive evaluation of NAA on Caryota mitis cold tolerance. Journal of Northwest Forestry University, 27(3), 39-43.
  [阮志平 (2012). 萘乙酸对短穗鱼尾葵耐寒性的影响. 西北林学院学报, 27(3), 39-43.]
[24] Ruan ZP (2014). Effects of NAA on physiological indexes of Caryota obtusa seedlings under variable temperature stress. Acta Agriculturae Zhejiangensis, 26, 609-614.
  [阮志平 (2014). 萘乙酸对董棕变温冷胁迫的生理效应. 浙江农业学报, 26, 609-614.]
[25] Shomali A, Das S, Sarraf M, Johnson R, Janeeshma E, Kumar V, Aliniaeifard S, Puthur JT, Hasanuzzaman M (2024). Modulation of plant photosynthetic processes during metal and metalloid stress, and strategies for manipulating photosynthesis-related traits. Plant Physiology and Biochemistry, 206, 108211. DOI: 10.1016/j.plaphy.2023.108211.
[26] Singh A, Kumar A, Kumar R, Prakash J, Kumar N, Verma AK (2024). Evaluation of salt tolerance in jamun (Syzygium cumini L. Skeels) using morpho-physiological traits and membership function analysis. Scientia Horticulturae, 326, 112742. DOI: 10.1016/j.scienta.2023.112742.
[27] Su DY, Du LC, Kong FL, Zhang DD, Yang H, Li XB, Xu YH, Li G (2023). Effects of exogenous calcium on physiological characteristics of Panax ginseng C. A. Meyer seedlings under low temperature stress. Lishizhen Medicine and Materia Medica Research, 34, 2229-2232.
  [苏德悦, 杜立财, 孔凡丽, 张丹丹, 杨浩, 李辛琲, 许永华, 李刚 (2023). 外源钙对低温胁迫后人参叶片生长和生理特性的影响. 时珍国医国药, 34, 2229-2232.]
[28] Sun WS, Chen WX, Zhu LX, Qin N, Liu YY, Qu HY, Zheng M, Sun MT, Ge CM (2019). Effects of low-temperature and gibberellin on seed germination characteristics of Polygonatum sibiricum. Shandong Agricultural Sciences, 51(11), 64-67.
  [孙文帅, 陈为序, 祝丽香, 秦宁, 刘盈盈, 曲洪瑶, 郑敏, 孙湄婷, 葛春妹 (2019). 低温和赤霉素对泰山黄精种子萌发特性的影响. 山东农业科学, 51(11), 64-67.]
[29] Wang CM, Yang YY, Chen NH, Zeng ZX, Ji SJ, Shan W, Kuang JF, Lu WJ, Su XG, Chen JY, Zhao YT (2022). Physiological and transcription analyses reveal regulatory pathways of 6-benzylaminopurine delaying leaf senescence and maintaining quality in postharvest Chinese flowering cabbage. Food Research International, 157, 111455. DOI: 10.1016/j.foodres.2022.111455.
[30] Wang J, Chang YX, Xu KD, Wang HX, Chen L (2013). Mitigating effects of leaf spraying with four plant growth regulators on rape seedling under low temperature stress. Chinese Journal of Tropical Crops, 34, 2348-2352.
  [王佳, 常云霞, 徐克东, 王红星, 陈龙 (2013). 几种植物生长调节剂对低温胁迫下油菜幼苗生长抑制的缓解效应. 热带作物学报, 34, 2348-2352.]
[31] Wang LT, Xu ZL, Yang LY, Duan SZ, Cui MK, Gong M (2015). Effects of four chemical regulators on chilling tolerance and photosynthetic characteristics of tobacco seedlings. Acta Botanica Boreali-Occidentalia Sinica, 35, 801-808.
  [王丽特, 徐照丽, 杨利云, 段胜智, 崔明昆, 龚明 (2015). 4种化学调控剂对烟草幼苗耐冷性及其光合特性的效应研究. 西北植物学报, 35, 801-808.]
[32] Wang LW, Li MQ, Li HN, Gao XN, Zhao SY, Zhao SW, He MH, Wang C (2023). Effects of low temperature stress and recovery on physiological characteristics of soybean during growth stages. Molecular Plant Breeding, https://kns.cnki.net/kcms/detail//46.1068.S.20230228.1531.015.html.
  [王立为, 李明倩, 李海楠, 高西宁, 赵思玉, 赵思文, 贺明慧, 王成 (2023). 低温胁迫及复温对大豆关键生育时期叶片生理特性的影响. 分子植物育种, https://kns.cnki.net/kcms/detail//46.1068.S.20230228.1531.015.html.]
[33] Wang XY, Liu DM, Wei MM, Man JG (2020). Spraying 6-BA could alleviate the harmful impacts of waterlogging on dry matter accumulation and grain yield of wheat. PeerJ, 8, e8193. DOI: 10.7717/peerj.8193.
[34] Wu P, Ma YD, Ahammed GJ, Hao BY, Chen JY, Wan WL, Zhao YH, Cui HM, Xu W, Cui JX, Liu HY (2022). Insights into melatonin-induced photosynthetic electron transport under low-temperature stress in cucumber. Frontiers in Plant Science, 13, 1029854. DOI: 10.3389/fpls.2022.1029854.
[35] Xiong BP, Lei NF, Liu JQ, Zhou JC, Luo Z (2022). Physiological response of ivy to exogenous abscisic acid under low temperature stress. Northern Horticulture, (3), 71-78.
  [熊炳平, 雷泞菲, 刘金渠, 邹佳城, 罗治 (2022). 低温胁迫下常春藤对外源脱落酸的生理响应. 北方园艺, (3), 71-78.]
[36] Xu HM, Ye C, Zhang B, Xu HL, Xin GR, Liao WB (2010). Structure and dynamic succession on mangrove communities from Techeng Island, Zhanjiang, Guangdong, China. Ecology and Environmental Sciences, 19, 864-869.
  [许会敏, 叶蝉, 张冰, 徐华林, 辛国荣, 廖文波 (2010). 湛江特呈岛红树林植物群落的结构和动态特征. 生态环境学报, 19, 864-869.]
[37] Yang DG, Gong L, Wang YB, He L, Sun H, Ren J, Zheng YQ, Song CR, Yu QJ, Zhai JY, Zhang Q, Xie TL, Li W (2023). Effects of exogenous 6-benzylaminopurine (6-BA) on cold resistance of maize seedlings. Journal of Maize Sciences, 31(5), 74-82.
  [杨德光, 宫磊, 王迎博, 何丽, 孙慧, 任静, 郑禹琪, 宋纯儒, 于庆江, 翟婧宜, 张倩, 谢腾龙, 李威 (2023). 外源6-苄氨基嘌呤(6-BA)对玉米幼苗抗冷性影响研究. 玉米科学, 31(5), 74-82.]
[38] Zahra N, Hafeez MB, Ghaffar A, Kausar A, Al Zeidi M, Siddique KHM, Farooq M (2023). Plant photosynthesis under heat stress: effects and management. Environmental and Experimental Botany, 206, 105178. DOI: 10.1016/j.envexpbot.2022.105178.
[39] Zhang WJ, Huang ZL, Xu KF, Liu L, Zeng YL, Ma SY, Fan YH (2019). The effect of plant growth regulators on recovery of wheat physiological and yield-related characteristics at booting stage following chilling stress. Acta Physiologiae Plantarum, 41, 133. DOI: 10.1007/s11738-019-2924-8.
[40] Zhang XQ, Hu MA, Wang XZ, Zhang XY (2023). The effects of chitosan oligosaccharides, bacillus and lentinan on the growth of cucumber seedlings and the effect of low temperature resistance. Jiangsu Agricultural Sciences, 51(18), 156-162.
  [张学青, 胡蒙爱, 王晓卓, 张雪艳 (2023). 壳寡糖、芽孢杆菌、香菇多糖复合对黄瓜幼苗生长和低温抗性的影响. 江苏农业科学, 51(18), 156-162.]
[41] Zhang XT, Zhuang Y, Dong JH, Yi CX, Zhou BY, Ouyang JZ (2023). Effects of brassinosteroids and vegetable oil mixed spraying on photosynthesis of lychee and lotus at low temperature. Fruit Trees of Southern China, 52(6), 76-84.
  [张晓婷, 庄赟, 董家辉, 易晨歆, 周碧燕, 欧阳建忠 (2023). 芸苔素和植物油混合喷施对低温下荔枝和莲雾光合作用影响. 中国南方果树, 52(6), 76-84.]
[42] Zhang XY, Yang YL, Li SJ, Xiong RC, Xiang H (2018). Effects of exogenous GA3 and 6-BA on leaf senescence in low temperature stress of virus-free potato cutting seedlings. Crops, (4), 95-101.
  [张晓勇, 杨友联, 李树江, 熊荣川, 向红 (2018). 外源激素对低温胁迫下脱毒马铃薯扦插苗早衰的影响. 作物杂志, (4), 95-101.]
[43] Zhang YB, Qin HB, Guo LF, Pan YL, Wang XG, Jiang XN (2024). Effects of soil conditions on photosynthesis and leaf nitrogen, phosphorus and potassium contents of blueberry. Fruit Trees of Southern China, 53(1), 143-149.
  [张怡彬, 秦洪波, 郭伦发, 潘燕林, 王新桂, 江新能 (2024). 土壤条件对蓝莓光合作用和叶片氮、磷、钾含量的影响. 中国南方果树, 53(1), 143-149.]
[44] Zhou QH, Bao ZY, Yu Y, Chen W, Yang ZF, Cao SF, Shi LY (2023). IAA regulated levels of endogenous phytohormones in relation to chilling tolerance in cold-stored peaches after harvest. Postharvest Biology and Technology, 205, 112490. DOI: 10.1016/j.postharvbio.2023.112490.
[45] Zhou YZ (2018). Effects of Different Growth Regulators on Main Physiological Characteristics and Yield of Maize Under Low Temperature Stress. Master degree dissertation, Yanbian University, Yanji, Jilin.
  [周雅卓 (2018). 低温胁迫下不同生长调节剂对玉米主要生理特性及产量的影响. 硕士学位论文, 延边大学, 吉林延吉.]
Options
文章导航

/

〈 〉

Copyright © 2026 版权所有 《植物生态学报》编辑部
地址: 北京香山南辛村20号, 邮编: 100093
Tel.: 010-62836134, 62836138;　Fax: 010-82599431;　E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn
备案号: 京ICP备16067583号-19