芍药组内不同类群间光合特性及叶绿素荧光特性比较

doi:10.3773/j.issn.1005-264x.2010.12.013

摘要/Abstract

摘要：

为了比较分析芍药组不同类群光合生理差异以及它们对各自不同光照环境的适应性, 测定了芍药组(Paeonia sect. Paeonia) 4种2变种的光合日变化、光响应曲线、CO₂响应曲线, 以及叶绿素荧光特性。结果显示, 芍药组中不同类群之间光合速率差异明显, 各个种类的“午休”程度不同, 芍药(Paeonia lactiflora )和毛果芍药(P. lactiflora var. trichocarpa)的强光抑制现象没有川赤芍(P. veitchii)、美丽芍药(P. mairei)和窄叶芍药(P. anomala)明显。叶绿素荧光特征能够反映芍药组不同类群光合生理的差异。芍药组内不同类群地理分布的差异能部分从光合生理特征的适应性方面得到解释。

关键词: CO₂响应曲线, 光合日变化, 光响应曲线, 芍药, 拟合模型

Abstract:

Aims Our aims are to compare the photosynthetic and fluorescence characteristics in different species in Paeonia sect. Paeonia and to determine how these species fit different circumstances in photosynthesis. This information will be important in protecting and using these wild resources.
Methods The LI-6400 Portable Photosynthesis System was used to determine diurnal photosynthetic changes, light-response curves and CO₂-response curves of different species in sect. Paeonia under good growth conditions. The OS5-FL modulated chlorophyll fluorometer was used to determine their chlorophyll fluorescence characteristics. The light-response curves and CO₂-response curves were simulated with modified rectangular and nonrectangular hyperbolic models.
Important findings We found an obvious difference between photosynthetic rates of species in sect. Paeonia. The simulated result with modified rectangular hyperbolic model is more consistent with the result determined than the other model. The index of light-response curves and CO₂-response curves differ among species. Paeonia veitchii, P. anomala, P. mairei, P. anomala var. intermedia and P. lactiflora have the characteristic of “siesta”, but it is not obvious in P. lactiflora var. trichocarpa. The index of chlorophyll fluorescence characteristics reflects the photosynthetic characteristics. The photosynthetic characteristics of P. lactiflora and P. lactiflora var. trichocarpa embody their strong adaptability.

Key words: CO₂-response curve, diurnal photosynthetic changes, light-response curve, Paeonia lactiflora, simulating model

简在友, 王文全, 孟丽, 许桂芳, 王秋玲, 李卫东, 俞敬波. 芍药组内不同类群间光合特性及叶绿素荧光特性比较. 植物生态学报, 2010, 34(12): 1463-1471. DOI: 10.3773/j.issn.1005-264x.2010.12.013

JIAN Zai-You, WANG Wen-Quan, MENG Li, XU Gui-Fang, WANG Qiu-Ling, LI Wei-Dong, YU Jing-Bo. Comparison of photosynthetic and fluorescence characteristics among taxa in Paeonia sect. Paeonia. Chinese Journal of Plant Ecology, 2010, 34(12): 1463-1471. DOI: 10.3773/j.issn.1005-264x.2010.12.013

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https://www.plant-ecology.com/CN/Y2010/V34/I12/1463

图/表 6

参考文献 13

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分类群 Taxon	地点 Location	海拔, 经纬度Elevation (m), latitude, longitude	生境, 分布Habitat, distribution	气候特征(年平均)
分类群 Taxon	地点 Location	海拔, 经纬度Elevation (m), latitude, longitude	生境, 分布Habitat, distribution	降雨量Rainfall (mm)	气温 Air temperature (℃)	大于10 ℃积温Accumulative temperature: above 10 ℃ (℃)
芍药 P. lactiflora	内蒙古多伦蔡木山 Caimushan, Duolun, Inner Mongolia	1 370, 42°24′16″ N 116°45′29″ E	草原灌丛, 聚群Brush, cluster	440	0.4	1 872
毛果芍药 P. lactiflora var. trichocarpa	宁夏隆德沙塘 Shatang, Longde, Ningxia	1 980, 35°35′45″ N, 105°58′54″ E	耕地, 栽培 Tilth, cluster	580	12.6	4 272
川赤芍 P. veitchii	陕西眉县汤峪 Tangyu, Meixian, Shaanxi	2 800, 34°08′15″ N 107°53′42″ E	林下, 阳坡, 聚群 Forest, sunny, cluster	700	11.9	3 768
窄叶芍药 P. anomala	新疆额敏168团 168 regiment, Emin, Xinjiang	1 480, 46°55′30″ N 84°28′27″ E	灌丛, 阳坡, 聚群 Brush, sunny, cluster	270	6.0	2 904
块根芍药 P. anomala var. intermedia	新疆额敏168团 168 regiment, Emin, Xinjiang	1 480, 46°55′30″ N 84°28′28″ E	灌丛, 阳坡, 聚群 Brush, sunny, cluster	270	6.0	2 904
美丽芍药 P. mairei	四川北川擂鼓 Leigu, Beichuan, Sichuan	1 600, 31°50′20″ N 104°21′40″ E	林下, 阳坡, 聚群 Forest, sunny, cluster	1 110	16.1	5 184
	北京·朝阳区 Chaoyang, Beijing	50, 39°58′52″ N 116°27′58″ E	市区 City	580	11.4	4 320

分类群 Taxon	地点 Location	海拔, 经纬度Elevation (m), latitude, longitude	生境, 分布Habitat, distribution	气候特征(年平均)
分类群 Taxon	地点 Location	海拔, 经纬度Elevation (m), latitude, longitude	生境, 分布Habitat, distribution	降雨量Rainfall (mm)	气温 Air temperature (℃)	大于10 ℃积温Accumulative temperature: above 10 ℃ (℃)
芍药 P. lactiflora	内蒙古多伦蔡木山 Caimushan, Duolun, Inner Mongolia	1 370, 42°24′16″ N 116°45′29″ E	草原灌丛, 聚群Brush, cluster	440	0.4	1 872
毛果芍药 P. lactiflora var. trichocarpa	宁夏隆德沙塘 Shatang, Longde, Ningxia	1 980, 35°35′45″ N, 105°58′54″ E	耕地, 栽培 Tilth, cluster	580	12.6	4 272
川赤芍 P. veitchii	陕西眉县汤峪 Tangyu, Meixian, Shaanxi	2 800, 34°08′15″ N 107°53′42″ E	林下, 阳坡, 聚群 Forest, sunny, cluster	700	11.9	3 768
窄叶芍药 P. anomala	新疆额敏168团 168 regiment, Emin, Xinjiang	1 480, 46°55′30″ N 84°28′27″ E	灌丛, 阳坡, 聚群 Brush, sunny, cluster	270	6.0	2 904
块根芍药 P. anomala var. intermedia	新疆额敏168团 168 regiment, Emin, Xinjiang	1 480, 46°55′30″ N 84°28′28″ E	灌丛, 阳坡, 聚群 Brush, sunny, cluster	270	6.0	2 904
美丽芍药 P. mairei	四川北川擂鼓 Leigu, Beichuan, Sichuan	1 600, 31°50′20″ N 104°21′40″ E	林下, 阳坡, 聚群 Forest, sunny, cluster	1 110	16.1	5 184
	北京·朝阳区 Chaoyang, Beijing	50, 39°58′52″ N 116°27′58″ E	市区 City	580	11.4	4 320

种类 Species	项目 Index	9:00	10:00	11:00	12:00	13:00	14:00	15:00	16:00	17:00	18:00	19:00
芍药 P. lactiflora	PAR	1 330	1 373	1 615	1 685	1 671	1 584	1 296	388	184	114	44
	T_l	33.71	38.24	41.12	41.40	39.39	42.57	38.19	34.41	32.45	31.47	30.41
	P_n	10.03^b	10.43^b	10.67^b	7.97^b	9.73^b	9.02^b	9.89^b	8.89^b	3.11^c	3.45^c	1.35^a
	T_r	3.18^d	2.51^c	4.44^b	4.30^b	2.19^b	2.75^c	1.24^c	0.70^c	0.08^c	-0.48^e	-0.61^c
川赤芍 P. veitchii	PAR	1 224	1 399	1 548	1 543	1 525	1 237	1 141	301	270	259	39
	T_l	33.40	37.49	41.52	40.47	38.95	41.01	37.80	34.18	32.78	32.01	30.35
	P_n	6.30^c	8.79^c	7.72^c	4.47^c	7.18^c	7.96^c	9.00^c	8.00^c	7.62^c	6.86^a	1.42^a
	T_r	3.85^b	3.31^b	3.83^c	3.50^c	2.16^b	3.86^b	2.10^b	1.19^b	0.73^a	0.79^a	-0.01^b
窄叶芍药 P. anomala	PAR	1 389	1 517	1 623	1 664	1 649	1 208	1 160	466	140	87	28
	T_l	34.78	38.55	41.25	41.83	39.33	41.22	38.33	34.83	32.06	31.74	30.52
	P_n	6.21^c	7.75^d	5.95^d	3.52^d	7.45^c	7.12^d	8.21^d	6.47^d	4.70^b	4.25^b	1.84^a
	T_r	3.48^c	1.31^e	1.52^e	1.13^e	0.59^d	1.15^e	0.63^d	0.37^d	-0.12^d	-0.25^d	-1.02^d
美丽芍药 P. mairei	PAR	1 497	1 603	1 704	1 650	1 538	1 090	946	242	93	43	16
	T_l	35.8	38.91	42.47	42.18	40.93	41.76	39.08	34.61	31.93	31.75	30.66
	P_n	4.45^d	7.05^e	5.64^e	2.19^e	6.03^d	6.3^e	7.25^e	5.10^e	4.37^b	3.86^c	-0.90^b
	T_r	3.47^c	1.99^d	2.40^d	2.09^d	1.25^c	1.98^d	0.58^d	0.35^d	-0.14^d	-0.06^c	0.69^a
毛果芍药 P. lacrtiflora var. trichocarpa	PAR	1 456	1 427	1 517	1 557	1 564	1 294	1 163	1 071	189	109	17
	T_l	35.2	37.92	40.66	40.62	40.03	39.6	38.11	35.60	31.68	31.38	30.70
	P_n	14.58^a	16.60^a	15.01^a	15.48^a	14.89^a	14.59^a	16.25^a	13.72^a	8.62^a	6.37^a	-1.02^b
	T_r	5.75^a	5.12^a	5.81^a	5.43^a	4.47^a	5.34^a	3.68^a	3.31^a	0.62^b	0.37^b	0.90^a

种类 Species	项目 Index	9:00	10:00	11:00	12:00	13:00	14:00	15:00	16:00	17:00	18:00	19:00
芍药 P. lactiflora	PAR	1 330	1 373	1 615	1 685	1 671	1 584	1 296	388	184	114	44
	T_l	33.71	38.24	41.12	41.40	39.39	42.57	38.19	34.41	32.45	31.47	30.41
	P_n	10.03^b	10.43^b	10.67^b	7.97^b	9.73^b	9.02^b	9.89^b	8.89^b	3.11^c	3.45^c	1.35^a
	T_r	3.18^d	2.51^c	4.44^b	4.30^b	2.19^b	2.75^c	1.24^c	0.70^c	0.08^c	-0.48^e	-0.61^c
川赤芍 P. veitchii	PAR	1 224	1 399	1 548	1 543	1 525	1 237	1 141	301	270	259	39
	T_l	33.40	37.49	41.52	40.47	38.95	41.01	37.80	34.18	32.78	32.01	30.35
	P_n	6.30^c	8.79^c	7.72^c	4.47^c	7.18^c	7.96^c	9.00^c	8.00^c	7.62^c	6.86^a	1.42^a
	T_r	3.85^b	3.31^b	3.83^c	3.50^c	2.16^b	3.86^b	2.10^b	1.19^b	0.73^a	0.79^a	-0.01^b
窄叶芍药 P. anomala	PAR	1 389	1 517	1 623	1 664	1 649	1 208	1 160	466	140	87	28
	T_l	34.78	38.55	41.25	41.83	39.33	41.22	38.33	34.83	32.06	31.74	30.52
	P_n	6.21^c	7.75^d	5.95^d	3.52^d	7.45^c	7.12^d	8.21^d	6.47^d	4.70^b	4.25^b	1.84^a
	T_r	3.48^c	1.31^e	1.52^e	1.13^e	0.59^d	1.15^e	0.63^d	0.37^d	-0.12^d	-0.25^d	-1.02^d
美丽芍药 P. mairei	PAR	1 497	1 603	1 704	1 650	1 538	1 090	946	242	93	43	16
	T_l	35.8	38.91	42.47	42.18	40.93	41.76	39.08	34.61	31.93	31.75	30.66
	P_n	4.45^d	7.05^e	5.64^e	2.19^e	6.03^d	6.3^e	7.25^e	5.10^e	4.37^b	3.86^c	-0.90^b
	T_r	3.47^c	1.99^d	2.40^d	2.09^d	1.25^c	1.98^d	0.58^d	0.35^d	-0.14^d	-0.06^c	0.69^a
毛果芍药 P. lacrtiflora var. trichocarpa	PAR	1 456	1 427	1 517	1 557	1 564	1 294	1 163	1 071	189	109	17
	T_l	35.2	37.92	40.66	40.62	40.03	39.6	38.11	35.60	31.68	31.38	30.70
	P_n	14.58^a	16.60^a	15.01^a	15.48^a	14.89^a	14.59^a	16.25^a	13.72^a	8.62^a	6.37^a	-1.02^b
	T_r	5.75^a	5.12^a	5.81^a	5.43^a	4.47^a	5.34^a	3.68^a	3.31^a	0.62^b	0.37^b	0.90^a

项目 Item	模型 Model	指标 Index		芍药 P. lactiflora	川赤芍 P. veitchii	美丽芍药 P. mairei	块根芍药 P. anomala var. intermedia	毛果芍药 P. lacrtiflora var. trichocarpa	窄叶芍药 P. anomala
光响应曲线 Light- response curve	观测Determined	PAR_max		1 499	1 600	1 000	1 800	1 501	1 000
	观测Determined	A_max		14.9	11.5	7.8	18.8	11.5	7.3
	模型1 Model 1	R_day		1.240	0.317	0.930	1.940	1.290	0.253
		LCP		16.0	6.0	13.7	26.0	21.0	5.0
		A_max		15.93	11.67	8.28	21.52	12.14	7.07
		Φ		0.049	0.028	0.036	0.050	0.036	0.047
		r		0.995	0.985	0.990	0.990	0.980	0.986
		K		0.820	0.960	0.932	0.774	0.872	0.996
		观测值与预测值相关性Correlation of observed and forecast (R)		0.995	0.984	0.990	0.989	0.981	0.972
	模型2 Model 2	R_day		2.14	1.08	1.54	4.85	1.62	3.04
		LCP		31.43	25.22	24.12	61.40	34.33	18.00
		LSP		1 300.0	1 176.5	941.7	1 700.0	1 174.0	669.0
		PLR		14.04	11.68	7.54	18.03	10.60	7.25
		r		0.997	0.980	0.990	0.990	0.980	0.980
		观测值与预测值相关性Correlation of observed and forecast (R)		0.997	0.980	0.991	0.990	0.981	0.980
		参数 Parameter	E	0.068	0.043	0.064	0.079	0.047	0.169
			M	0.000 14	0.000 22	0.00016	0.000 10	0.000 17	0.000 14
			N	0.003 0	0.001 8	0.006 0	0.003 0	0.002 6	0.019 0
CO₂响应曲线CO₂-response curve	观测Determined	PAR_max		1 575	975	2 046	966	1 155	1 547
	观测Determined	A_max		19.8	18.4	5.4	24.4	29.5	17.6
	模型1 Model 1	R_day		2.155	2.670	0.747	2.270	2.530	2.485
		CCP		34	40	52	29	41	43
		CSP		20.080	18.120	6.374	26.930	30.100	19.900
		r		0.993	0.960	0.995	0.998	0.992	0.995
		观测值与预测值相关性Correlation of observed and forecast (R)		0.993	0.955	0.996	0.877	0.993	0.995
		参数 Parameter	Φ	0.033 0	0.034 0	0.008 3	0.041 0	0.033 0	0.030 0
		参数 Parameter	K	0.985	0.990	0.891	0.979	0.990	0.961
	模型2 Model 2	R_day		3.120	4.270	0.998	5.560	4.400	3.060
		CCP		72.29	79.35	92.98	85.21	95.70	77.20
		CSP		1 299	1 031	1 860	1 026	1 264	1 392
		PCR		20.12	17.95	5.29	23.59	23.06	17.44
		r		0.996	0.988	0.994	0.995	0.994	0.998
		观测值与预测值相关性Correlation of observed and forecast (R)		0.996	0.988	0.994	0.995	0.995	0.999
		参数 Parameter	E	0.043	0.054	0.011	0.065	0.046	0.040
			M	0.000 32	0.000 36	0.000 17	0.000 42	0.000 36	0.000 27
			N	0.000 42	0.000 76	0.000 78	0.000 45	0.000 20	0.000 63