Chin J Plan Ecolo ›› 2017, Vol. 41 ›› Issue (5): 570-576.doi: 10.17521/cjpe.2016.0245

• Research Articles • Previous Articles     Next Articles

Effects of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Hydrangea macrophylla

Jian-Guo CAI*, Meng-Qi WEI, Yi ZHANG, Yun-Long WEI   

  1. School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China
  • Online:2017-06-22 Published:2017-05-10
  • Contact: Jian-Guo CAI
  • About author:

    KANG Jing-yao(1991-), E-mail: kangjingyao_nj@163.com

Abstract:

Aims The objectives were to investigate the effects of different light intensities on photosynthetic characteristics and chlorophyll fluorescence parameters, to clarify the physiological responses and photo-protective mechanisms of Hydrangea macrophylla to changes in light regimes in view of the distribution of energy absorbed and photosynthetic characteristics.Methods Three light regimes including natural and shade (shading rate 50% and 75% of natural light) were applied to plants for 60 days. After the treatment, the gas-exchange, chlorophyll a fluorescence and photosynthesis-light curves were measured by a portable leaf gas exchange system (LI-6400).Important findings The results showed that the weak light intensity treatment reduced dark respiration rate, light compensation point and light saturation point of plant, but increased apparent quantum yield, suggesting that plants had the physiological strategy to utilize the weakening light by reducing respiration. The net photosynthetic rate, intercellular CO2 concentration, transpiration rate and water use efficiency of plants grown below 50% of natural light showed significant difference compared with natural and shading rate 75% of natural light. There were significant difference between natural and shade treatments in the maximal quantum efficiency of PSII (Fv/Fm), as indicated that it was significantly less at full light than that at 50% of natural light. Initial fluorescence intensity (Fo) of plants was higher at full light than that at 50% of natural light, suggesting that photoinhibition occurred in natural light. The non-photochemical quenching (NQP) decreased with the aggravation of shade stress, indicating that shading decreased the efficiency of photochemical reaction by reducing the fraction of incident light in photochemical energy utilization and decreased thermal dissipation through regulating energy distribution in photosystem II (PSII) in the leaves of Hydrangea macrophylla. In general, the 70% of incident light in photochemical energy utilization was distributed to thermal dissipation, 20% was distributed to non-regulated energy dissipation and 4% was distributed to effective photochemical reaction. In conclusion, responses of plants to increased irradiance are governed by strategy: to utilize a high fraction of incident light in photochemistry and regulate energy dissipation in PSII and weaken the accumulation of excess excitation energy in PSII to protect the photosynthetic apparatus in the leaves of H. macrophylla under saturated radiation.

Key words: Hydrangea macrophylla, shading, photosynthesis, chlorophyll fluorescence, light adaptation

Fig. 1

Light response curves of net photosynthetic rate (Pn) in Hydrangea macrophylla under different light treatments (mean ± SD)."

Table 1

Effects of light treatments on photosynthesis-light response parameters of Hydrangea macrophylla"

参数
Parameter
全光
Full light
遮光率50%
Shading rate 50%
遮光率75%
Shading rate 75%
表观量子效率
AQY (mmol CO2·mol-1 photos)
0.03 0.04 0.03
最大净光合速率
Pnmax (μmolCO2·m-2·s-1)
3.50 3.85 3.83
暗呼吸速率
Rd (μmolCO2·m-2·s-1)
0.53 0.36 0.41
光补偿点
LCP (μmol·m-2·s-1)
15.75 6.71 8.00
光饱和点
LSP (μmol·m-2·s-1s-1)
307.42 278.14 281.57
相关系数 r 0.919 0.908 0.948

Table 2

Effects of light treatments on the gas exchange parameters of Hydrangea macrophylla (mean ± SD)"

处理
Treatment
参数 Parameter
Pn (μmol CO2·m-2·s-1) Ci ( μmol·mol-1) Gs (mol·m-2·s-1) Tr (mmol·m-2·s-1) WUE (mmol CO2·mol-1H2O)
全光 Full light 1.64 ± 0.34a 326.55 ± 21.45a 0.04 ± 0.004b 0.92 ± 0.11a 1.82 ± 0.53a
遮光率50% Shading rate 50% 2.14 ± 0.38b 300.46 ± 17.85b 0.04 ± 0.009b 1.40 ± 0.22b 1.54 ± 0.27b
遮光率75% Shading rate 75% 1.73 ± 0.28a 293.99 ± 18.33b 0.02 ± .009a 0.86 ± 0.26a 2.11 ± 0.47a

Table 3

Effects of light treatments on chlorophyll a fluorescence parameters of Hydrangea macrophylla (mean ± SD)"

参数 Parameter 处理 Treatment
全光 Full light 遮光率50% Shading rate 50% 遮光率75% Shading rate 75%
PSⅡ原初光能转化效率 Fv/Fm 0.63 ± 0.03b 0.67 ± 0.01c 0.60 ± 0.04a
PSⅡ潜在活性 Fv/Fo 1.72 ± 0.21b 2.00 ± 0.08c 1.51 ± 0.20a
初始荧光 Fo 786.59 ± 13.23b 770.21 ± 21.12a 836.86 ± 28.73b
PSII实际光量子产量 v/m 0.25 ± 0.01a 0.26 ± .04a 0.28 ± 0.06a
非光化学淬灭系数 NPQ 2.39 ± 0.16c 1.95 ± 0.09b 1.41 ± 0.34a
光化学淬灭系数 qP 0.17 ± 0.04a 0.18 ± 0.02a 0.15 ± 0.03a
电子传递速率 ETR 19.43 ± 3.88a 20.31 ± 5.53a 18.57 ± 4.50a

Table 4

The distribution of light energy absorbed in Hydrangea macrophylla under different light intensities"

处理
Treatment
能量分配 Energy distribution
天线热耗散
Fraction of photons dissipated
in the antenna (%)
光化学反应耗散
Fraction of photons utilized
in PSII photochemistry (%)
非光化学反应耗散
Fraction of absorbed photons by PSII
neither used in photochemistry nor
dissipated in the PSII (%)
全光 Full light 74.55 ± 5.32 4.44 ± 0.89 21.01 ± 1.12
遮阴50% Shading 50% 74.22 ± 4.50 4.65 ± 1.27 21.12 ± 3.34
遮阴75% Shading 75% 74.55 ± 5.32 4.24 ± 1.02 23.64 ± 6.13
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