Chin J Plan Ecolo ›› 2010, Vol. 34 ›› Issue (6): 695-703.doi: 10.3773/j.issn.1005-264x.2010.06.009

• Research Articles • Previous Articles     Next Articles

Systemic induced effects of mechanical wounding on the chemical defense of Bt corn (Zea mays)

FENG Yuan-Jiao1,2,3; JIN Qiong1,2,3; and WANG Jian-Wu1,2,3*   

  1. 1Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China;
    2Key Laboratory of Ecological Agriculture of Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;
    3Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China
  • Received:2009-07-10 Online:2010-06-01 Published:2010-06-01
  • Contact: WANG Jian-Wu

Abstract: Aims The major objective was to explore the difference in the systematic induced effects of mechanical wounding on Bt corn and conventional corn after the first leaf of corn seedlings was mechanically wounded.
Methods Two Bt corns, ‘5422Bt1’ (Bt11) and ‘5422CBCL’ (Mon810), as well as their conventional corn ‘5422’ were used to detect changes in the content of direct defense chemical DIMBOA and expression of its genes (Bx1, Bx6 and Bx9), content of direct defense chemical total phenol and expression of its gene (PAL), direct defense protein genes (MPI and PR-2a), and the indirect defense chemical volatile genes (FPS and TPS) in the non-wounded parts (second leaf and roots) by employing chemical analysis and gene expression analysis methods after the first leaf of corn seedlings was mechanically wounded for 6 h.
Important findings Mechanical wounding systematically induced the expression of PAL and TPS in the second leaf and PAL and FPS in the roots of conventional corn ‘5422’ as compared with the healthy plants, which significantly increased the DIMBOA content in the second leaf but reduced the DIMBOA content and its gene expression of Bx6 and Bx9 in the roots. In addition, mechanical wounding systematically induced the gene expression of Bx6, PAL, PR-2a and TPS in the second leaf and Bx6, Bx9, PAL, MPI, PR-2a and TPS in the roots of Bt corn ‘5422Bt1’, resulting in increased DIMBOA content of the second leaf. In terms of ‘5422CBCL’, mechanical wounding systematically induced the gene expression of Bx9, PAL, PR-2a and TPS in the second leaf and the gene expression of Bx6, PAL, MPI and TPS in the roots, which remarkably increased the total phenol content in the roots. The systematic induced effect of mechanical wounding on the chemical defense response is more obvious in the two Bt corns as compared with the conventional corn ‘5422’, suggesting that there is a synergistic relationship between the Bt gene introduction and chemical defense response during the systematic induced defense processes inflicted by mechanical wounding.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Chne Fu-heng and Fan Jun-shen. [J]. Chin Bull Bot, 1988, 5(02): 127 .
[2] . [J]. Chin Bull Bot, 1994, 11(专辑): 66 .
[3] Zhou Guang-sheng Xing Xue-rong Wang Hui-min. Feedback of Forest on Climate[J]. Chin Bull Bot, 1995, 12(专辑2): 190 -194 .
[4] ZHOU Qing;YANG Jing;SHAO Ai-Hua and WANG Ya-Ling. Effect of NaHSO3 on Root Growth and The Physiological Characteristics in Rice Seedling[J]. Chin Bull Bot, 1998, 15(03): 51 -53 .
[5] HAN Yan-Lai XU Fang-Sen DUAN Hai-Yan SHI Lei WANG Yun-Hua. Advances in Study on Proteins which Transport Nutrients in Arabidopsis thaliana[J]. Chin Bull Bot, 2003, 20(01): 23 -35 .
[6] Jie Wu, Xin Zhao, Wei Ning. Micro-morphological Characteristics of Taraxacum F. H. Wigg. Seeds from Northeastern China and Taxonomic Significance[J]. Chin Bull Bot, 2011, 46(4): 437 -446 .
[7] Miao Dong, Yue Huang, Wenduo Chen, Tao Xu, Qiulei Lang. Use of Degradome Sequencing in Study of Plant MicroRNAs[J]. Chin Bull Bot, 2013, 48(3): 344 -353 .
[8] Xia Yi-shen and Shao Tin-fu. Advances in Study on Physiology and Biochemistry of Fruit Ripening[J]. Chin Bull Bot, 1989, 6(01): 5 -8 .
[9] CHONG Yun-Xiao, YU Dan, XIA Sheng-Lin, KANG Hui. A Preliminary Study on the Floristic Geography of Aquatic Macrophytes and Helophytes in Taibai County, Shaanxi Province[J]. Chin J Plan Ecolo, 1999, 23(199901): 28 -38 .
[10] . [J]. Chin J Plan Ecolo, 1963, (2): 156 -157 .